scholarly journals Leptin activates ATP-sensitive potassium channels in the rat insulin-secreting cell line, CRI-G1

1997 ◽  
Vol 504 (3) ◽  
pp. 527-535 ◽  
Author(s):  
J. Harvey ◽  
F. McKenna ◽  
P. S. Herson ◽  
D. Spanswick ◽  
M. L. J. Ashford
Keyword(s):  
Potassium Channels ◽  
Cell Line ◽  
Secreting Cell

Role of tyrosine kinase in insulin release in an insulin secreting cell line (INS-1)

1995 ◽  
Vol 7 (5) ◽  
pp. 505-512 ◽  
Author(s):  
E.J. Verspohl ◽  
B. Tollkühn ◽  
H. Kloss
Keyword(s):  
Tyrosine Kinase ◽  
Cell Line ◽  
Insulin Release ◽  
Secreting Cell

alpha-Glycerophosphate shuttle in a clonal beta-cell line

1989 ◽  
Vol 256 (1) ◽  
pp. E173-E178 ◽  
Author(s):  
M. D. Meglasson ◽  
K. M. Smith ◽  
D. Nelson ◽  
M. Erecinska
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Electron Transport Chain ◽  
Direct Evidence ◽  
Mitochondrial Fraction ◽  
Secreting Cell ◽  
Transport Chain ◽  
Beta Cell Line ◽  
Atp Generation ◽  
Hit Cells

It has been proposed that the alpha-glycerophosphate (alpha-GOP) shuttle plays a crucial role in regulation of glycolysis in beta-cells by linking reoxidation of cytosolic NADH to formation of ATP in the electron transport chain (J. Biol. Chem. 265: 8287, 1981). Direct evidence for this suggestion is still lacking, however. In this work the operation of the alpha-GOP shuttle was investigated in the insulin-secreting cell line HIT-T15. The constituent enzymes of the pathway were found to be present in HIT cells. Flavin-linked alpha-GOP dehydrogenase was associated with the mitochondrial fraction, whereas NAD+-dependent alpha-GOP dehydrogenase was localized in the cytosol. In the presence of amobarbital (used to preserve the function of the alpha-GOP shuttle under conditions where oxidation of NADH by the respiratory chain was blocked), glucose increased insulin secretion, O2 consumption, and the cell [ATP]/[ADP] when compared with amobarbital alone. These results indicate that the alpha-GOP shuttle contributes to ATP generation in HIT cells and that its activation may be necessary for the initiation of insulin secretion by glucose.

Characterization of a Novel Glucose-Responsive Insulin-Secreting Cell Line, BRIN-BD11, Produced by Electrofusion

Diabetes ◽  
1996 ◽  
Vol 45 (8) ◽  
pp. 1132-1140 ◽  
Author(s):  
N. H. McClenaghan ◽  
C. R. Barnett ◽  
E. Ah-Sing ◽  
Y. H. A. Abdel-Wahab ◽  
F. P. M. O'Harte ◽  
...  
Keyword(s):  
Cell Line ◽  
Secreting Cell

ATP-sensitive potassium channels and efaroxan-induced insulin release in the electrofusion-derived BRIN-BD11 beta-cell line

Diabetes ◽  
1999 ◽  
Vol 48 (12) ◽  
pp. 2349-2357 ◽  
Author(s):  
J. C. Chapman ◽  
N. H. McClenaghan ◽  
K. E. Cosgrove ◽  
M. N. Hashmi ◽  
R. M. Shepherd ◽  
...  
Keyword(s):  
Potassium Channels ◽  
Beta Cell ◽  
Cell Line ◽  
Insulin Release ◽  
Beta Cell Line

Overexpression of glucagon-like peptide-1 receptor in an insulin-secreting cell line enhances glucose responsiveness

1997 ◽  
Vol 130 (1-2) ◽  
pp. 109-117 ◽  
Author(s):  
C Montrose-Rafizadeh ◽  
Y Wang ◽  
A.M Janczewski ◽  
T.E Henderson ◽  
J.M Egan
Keyword(s):  
Cell Line ◽  
Secreting Cell ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1 ◽  
Glucose Responsiveness

Long-term cross-species brain transplantation of a polymer-encapsulated dopamine-secreting cell line

1991 ◽  
Vol 111 (3) ◽  
pp. 269-275 ◽  
Author(s):  
Patrick Aebischer ◽  
Patrick A. Tresco ◽  
Shelley R. Winn ◽  
Lloyd A. Greene ◽  
Christine B. Jaeger
Keyword(s):  
Cell Line ◽  
Secreting Cell
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