Schwann Cell Marker Defined by a Monoclonal Antibody (224?58) with Species Cross-Reactivity. II. Molecular Characterization of the Epitope

Expression of the avian antigen SMP (Schwann cell Myelin Protein, Mr 75-80000), first characterized in the PNS with a monoclonal antibody as an early and strictly specific Schwann cell marker, was further studied in the CNS. Comparing SMP immunoreactive areas in the different parts of the CNS with those expressing the Myelin Basic Protein (MBP), we showed a strict colocalisation of both phenotypes. In vitro, MBP+ oligodendrocytes express the surface antigen SMP as well. SMP cellular expression was followed in situ and in culture using nervous tissues from embryos at different stages. We were thus able to detect an early expression of this marker by oligodendroblasts before the first appearance of MBP immunoreactivity. We have also identified a subpopulation of SMP+/MBP- and SMP+/GC- cells, which persists under our culture conditions as precursors remaining in an immature state.

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Molecular characterization of Euglena ascorbate peroxidase using monoclonal antibody

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/0304-4165(96)00002-5 ◽

1996 ◽

Vol 1290 (1) ◽

pp. 69-75 ◽

Cited By ~ 29

Author(s):

T Ishikawa

Keyword(s):

Monoclonal Antibody ◽

Molecular Characterization ◽

Ascorbate Peroxidase

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Molecular characterization of the gene locus of the human cell proliferation-associated nuclear protein defined by monoclonal antibody Ki-67

Cell Proliferation ◽

10.1046/j.1365-2184.1996.d01-2.x ◽

1996 ◽

Vol 29 (1) ◽

pp. 1-12 ◽

Cited By ~ 6

Author(s):

M. Duchrow ◽

C. Schluter ◽

C. Wohlenberg ◽

H.-D. Flad ◽

J. Gerdes

Keyword(s):

Monoclonal Antibody ◽

Cell Proliferation ◽

Molecular Characterization ◽

Human Cell ◽

Gene Locus ◽

Nuclear Protein ◽

Ki 67

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Molecular characterization of a human monoclonal antibody to B antigen in ABO blood type

Immunology Letters ◽

10.1016/s0165-2478(02)00294-8 ◽

2003 ◽

Vol 86 (1) ◽

pp. 45-51 ◽

Cited By ~ 7

Author(s):

Mika Kaneko ◽

Yukinari Kato ◽

Hidekazu Horiuchi ◽

Motoki Osawa

Keyword(s):

Monoclonal Antibody ◽

Molecular Characterization ◽

Human Monoclonal Antibody ◽

Blood Type ◽

Abo Blood Type

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Molecular characterization of an human monoclonal antibody that interacts with a sulfated tyrosine-containing epitope of the GPIb receptor and inhibits platelet functions

Molecular Immunology ◽

10.1016/j.molimm.2005.03.001 ◽

2006 ◽

Vol 43 (5) ◽

pp. 443-453 ◽

Cited By ~ 3

Author(s):

Yocheved Hagay ◽

Judith Lahav ◽

Avigdor Levanon ◽

David Varon ◽

Alex Brill ◽

...

Keyword(s):

Monoclonal Antibody ◽

Molecular Characterization ◽

Human Monoclonal Antibody ◽

Platelet Functions

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Characterization of G12 Sandwich ELISA, a Next-Generation Immunoassay for Gluten Toxicity

Journal of AOAC International ◽

10.5740/jaoacint.sge_halbmayr-jech ◽

2012 ◽

Vol 95 (2) ◽

pp. 372-376 ◽

Cited By ~ 17

Author(s):

Elisabeth Halbmayr-Jech ◽

Elisabeth Hammer ◽

Richard Fielder ◽

Jacqueline Coutts ◽

Adrian Rogers ◽

...

Keyword(s):

Monoclonal Antibody ◽

Celiac Disease ◽

Working Group ◽

Sandwich Elisa ◽

Extraction Methods ◽

Cross Reactivity ◽

Next Generation ◽

Preliminary Results ◽

Sample Extraction

Abstract In this work, a monoclonal antibody called G12, raised against the most immunotoxic peptide to celiac disease patients, was used to develop a sandwich ELISA. Preliminary results on cross-reactivities, recoveries, and extraction methods of the new assay are presented. The assay calibration was performed using material from the Prolamin Working Group. The antibody's specificity was determined by cross-reactivity studies on different grains, nuts, oils, and starches. Recovery of the assay was determined by spiking experiments on common food matrixes, as well as on problematic matrixes. Furthermore, sample extraction methods using ethanol, cocktail solution, and a proprietary buffer have been compared.

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