Cholera Toxin and Dibutyryl Cyclic AMP Inhibit the Expression of Neurofilament Protein Induced by Nerve Growth Factor in Cultures of Naive and Primed PC12 Cells

1987 ◽  
Vol 49 (6) ◽  
pp. 1676-1687 ◽  
Author(s):  
Patrick Doherty ◽  
Derek A. Mann ◽  
Frank S Walsh
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cyclic Amp ◽  
Cholera Toxin ◽  
Pc12 Cells ◽  
Dibutyryl Cyclic Amp ◽  
Neurofilament Protein ◽  
Nerve Growth

Cell-cell interactions modulate the responsiveness of PC12 cells to nerve growth factor

Development ◽  
1987 ◽  
Vol 101 (3) ◽  
pp. 605-615
Author(s):  
P. Doherty ◽  
D.A. Mann ◽  
F.S. Walsh
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Neuronal Cell ◽  
Cell Types ◽  
Glioma Cell Line ◽  
Protein Subunit ◽  
Neurofilament Protein ◽  
Nerve Growth ◽  
Cell Cell

The growth of PC12 cells on a collagen substratum or on monolayers of several non-neuronal cell types was studied by measuring nerve growth factor (NGF)-dependent increases in the expression of a 150 X 10(3) (Mr) neurofilament protein subunit and the membrane glycoprotein Thy-1. Both responses were found to be greatly suppressed in cultures of fibroblasts as compared to the C2 and G8-1 muscle cell lines and the C6 glioma cell line. This suppression was associated with an inhibition of NGF-dependent neuritic outgrowth from PC12 cells grown on fibroblast monolayers. There was no evidence that fibroblasts secrete soluble molecules that directly inhibit these responses or neutralize NGF. In addition, there was no difference in the neurofilament protein response from PC12 cells that had been treated with NGF prior to coculture, and the now primed PC12 cells readily extended axons over fibroblast monolayers. These data demonstrate that cell-cell and/or cell-matrix interactions can modulate biochemical responses to NGF and suggest that responsiveness of neuronal cells to environmental cues is not immutable. Control of the latter may be at the level of expression of receptor molecules for cell-surface- or matrix-associated macromolecules and a similar mechanism operating during development could play a role in growth cone guidance.

Effect of Nerve Growth Factor and Dibutyryl Cyclic AMP on Biopterin and Catecholamine Levels of PC12h Pheochromocytoma Cells

Pteridines ◽  
1991 ◽  
Vol 3 (1-2) ◽  
pp. 67-68
Author(s):  
N. Nakanishi ◽  
S. Onozawa ◽  
R. Matsumoto ◽  
M. Nakamura ◽  
S. Imagawa ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cyclic Amp ◽  
Dibutyryl Cyclic Amp ◽  
Nerve Growth ◽  
Pheochromocytoma Cells ◽  
Catecholamine Levels

Mechanisms of apoptosis in PC12 cells irreversibly differentiated with nerve growth factor and cyclic AMP

1999 ◽  
Vol 821 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Nathalie Lambeng ◽  
Patrick P Michel ◽  
Bernard Brugg ◽  
Yves Agid ◽  
Merle Ruberg
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cyclic Amp ◽  
Pc12 Cells ◽  
Nerve Growth
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