UPTAKE OF NORADRENALINE BY ADRENERGIC NERVES, SMOOTH MUSCLE AND CONNECTIVE TISSUE IN ISOLATED PERFUSED ARTERIES AND ITS CORRELATION WITH THE VASOCONSTRICTOR RESPONSE

Airway remodeling describes the structural changes that occur in the asthmatic airway that include airway smooth muscle hyperplasia, increases in vascularity due to angiogenesis, and thickening of the basement membrane. Our aim in this study was to examine the effect of transforming growth factor-β on the release of connective tissue growth factor and vascular endothelial growth factor from human airway smooth muscle cells derived from asthmatic and nonasthmatic patients. In addition we studied the immunohistochemical localization of these cytokines in the extracellular matrix after stimulating bronchial rings with transforming growth factor-β. Connective tissue growth factor and vascular endothelial growth factor were released from both cell types and colocalized in the surrounding extracellular matrix. Prostaglandin E2 inhibited the increase in connective tissue growth factor mRNA but augmented the release of vascular endothelial growth factor. Matrix metalloproteinase-2 decreased the amount of connective tissue growth factor and vascular endothelial growth factor, but not fibronectin deposited in the extracellular matrix. This report provides the first evidence that connective tissue growth factor may anchor vascular endothelial growth factor to the extracellular matrix and that this deposition is decreased by matrix metalloproteinase-2 and prostaglandin E2. This relationship has the potential to contribute to the changes that constitute airway remodeling, therefore providing a novel focus for therapeutic intervention in asthma.

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The penis in diabetes: structural analysis of connective tissue and smooth muscle alterations in a rabbit model

BJU International ◽

10.1111/j.1464-410x.2010.09944.x ◽

2010 ◽

Vol 108 (3) ◽

pp. 400-404 ◽

Cited By ~ 17

Author(s):

Marcelo Abidu-Figueiredo ◽

Ilma C. Ribeiro ◽

Mauricio A. Chagas ◽

Luiz E. M. Cardoso ◽

Waldemar S. Costa ◽

...

Keyword(s):

Smooth Muscle ◽

Structural Analysis ◽

Connective Tissue ◽

Rabbit Model

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Immunological Properties of Connective Tissue and Smooth Muscle Cells

Advances in Experimental Medicine and Biology - Arterial Mesenchyme and Arteriosclerosis ◽

10.1007/978-1-4684-3243-5_17 ◽

1974 ◽

pp. 335-353 ◽

Cited By ~ 6

Author(s):

J. Renais ◽

P. Hadjiisky ◽

L. Scebat

Keyword(s):

Smooth Muscle ◽

Connective Tissue ◽

Smooth Muscle Cells ◽

Muscle Cells ◽

Immunological Properties

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Connective Tissue Disorders and Smooth Muscle Disorders in Cardiology

Clinical Cardiogenetics ◽

10.1007/978-1-84996-471-5_18 ◽

2010 ◽

pp. 263-282 ◽

Cited By ~ 1

Author(s):

K. van Engelen ◽

B. J. M. Mulder

Keyword(s):

Smooth Muscle ◽

Connective Tissue ◽

Connective Tissue Disorders ◽

Muscle Disorders

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Melatonin potentiates contractile responses to serotonin in isolated porcine coronary arteries

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.2001.280.1.h76 ◽

2001 ◽

Vol 280 (1) ◽

pp. H76-H82 ◽

Cited By ~ 20

Author(s):

Qiong Yang ◽

Elizabeth Scalbert ◽

Philippe Delagrange ◽

Paul M. Vanhoutte ◽

Stephen T. O'Rourke

Keyword(s):

Nitric Oxide ◽

Methylene Blue ◽

Smooth Muscle ◽

Coronary Arteries ◽

Isometric Tension ◽

Maximal Response ◽

Melatonin Receptor ◽

Vasomotor Tone ◽

Vasoconstrictor Response ◽

Coronary Vasomotor Tone

The present study was designed to determine the effects of melatonin on coronary vasomotor tone. Porcine coronary arteries were suspended in organ chambers for isometric tension recording. Melatonin (10−10-10−5 M) itself caused neither contraction nor relaxation of the tissues. Serotonin (10−9-10−5 M) caused concentration-dependent contractions of coronary arteries, and in the presence of melatonin (10−7 M) the maximal response to serotonin was increased in rings with but not without endothelium. In contrast, melatonin had no effect on contractions produced by the thromboxane A2 analog U-46619 (10−10-10−7 M). The melatonin-receptor antagonist S-20928 (10−6 M) abolished the potentiating effect of melatonin on serotonin-induced contractions in endothelium-intact coronary arteries, as did treatment with 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (10−5 M), methylene blue (10−5 M), or NG -nitro-l-arginine (3 × 10−5 M). In tissues contracted with U-46619, serotonin caused endothelium-dependent relaxations that were inhibited by melatonin (10−7 M). Melatonin also inhibited coronary artery relaxation induced by sodium nitroprusside (10−9-10−5 M) but not by isoproterenol (10−9-10−5 M). These results support the hypothesis that melatonin, by inhibiting the action of nitric oxide on coronary vascular smooth muscle, selectively potentiates the vasoconstrictor response to serotonin in coronary arteries with endothelium.

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Immunocytochemical characteristics of submucosal uterine myomas

Vojnosanitetski pregled ◽

10.2298/vsp1012977m ◽

2010 ◽

Vol 67 (12) ◽

pp. 977-982 ◽

Cited By ~ 1

Author(s):

Aleksandra Mladenovic-Mihailovic ◽

Zorica Mladenovic-Bogdanovic ◽

Predrag Mitrovic ◽

Irena Tanaskovic ◽

Slavica Usaj-Knezevic ◽

...

Keyword(s):

Smooth Muscle ◽

Connective Tissue ◽

Smooth Muscle Cells ◽

Smooth Muscle Actin ◽

Muscle Cells ◽

Synthetic Activity ◽

Benign Tumors ◽

Paraffin Sections ◽

Weak Reaction ◽

Synthetic Phenotype

Background/Aim. Myomas of the uterus, the most common benign tumors, have been studied for decades from the aspects of different basic and clinical disciplines. Despite this fact, their pathogenesis is still poorly understood. The aim of this study was to determine immunocytochemical characteristics of smooth muscle cells and connective tissue components of submucosal myomas of the uterus. Method. During the course of this study, 25 samples of submucosal myomas of the uterus were analyzed, all of them obtained during the surgery, after abdominal histerctomy by Aldridge. The samples were fixed in 4% formalin and embedded in paraffin. Sections of 5 ?m thickness were stained immunocytochemically using the DAKO LSAB+/HRP technique to identify ?- smooth muscle actin (?-SMA), vimentin, desmin, CD34, CD45, CD68 and PCNA (DAKO specification). Results. Our results suggest that submucosal myomas of the uterus are build-up of smooth muscle cells which are immunoreactive to ?-SMA and desmin, but also to a certain number of smooth muscle cells which are immunoreactive to ?-SMA and vimentin. Some of vimentin-immunoreactive cells also show an immunoreactivity of PCNA. In the build-up of connective stroma CD34-immunoreactive fibroblasts and neovascular formations are also present. By examining the distribution of CD45 antigen, at all the analyzed samples we observed a weak reaction. Conclusion. Submucosal myomas of the uterus are made-up of smooth muscle cells of the highly differentiated contractile phenotype (?-SMA- and desminimmunoreactivity), as well as smooth muscle cell of the synthetic phenotype which proliferate (?-SMA-, vimentin- and PCNA-immunoreactivity). In submucosal myoma of the uterus there is a significant presence of connective tissue as a result of synthetic activity of fibroblasts, which clearly differ in their immunocytochemical characteristics from smooth muscle cells of the synthetic phenotype.

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