Characterization of the biological effects of 2-methylthio-ATP on rat hepatocytes: clear-cut differences with ATP

Three xenobiotic amphipathic carboxylates, namely MEDICA 16, nafenopin and bezafibrate, which differ remarkably in their hydrophobic backbones, were found to acylate membrane and cytosolic liver proteins in cultured rat hepatocytes. The acylation patterns observed were time- and dose-dependent, and the acylated residue consisted of the original xenobiotic. The acylation patterns generated by the three xenobiotic carboxylates included common proteins which were acylated by the three xenobiotics (e.g. proteins of 32, 52, 56 and 72 kDa) as well as unique proteins which were specifically acylated by the respective xenobiotics. The acylation of liver proteins by either MEDICA 16 or nafenopin remained unaffected under conditions where protein synthesis was completely inhibited by cycloheximide. Protein acylation thus offers a common mode of action of xenobiotic amphipathic carboxylates, which may, however, result in diverse xenobiotyl-protein adducts. The xenobiotyl-acylated proteins might be involved in triggering some of the biological effects exerted by xenobiotic amphipathic carboxylates employed as hypolipidaemic effectors, peroxisomal proliferators or preadipocyte convertors.

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CHARACTERIZATION OF A HEPATOCYTE CELL LINE AND PRIMARY RAT HEPATOCYTES CULTURED THREE-DIMENSIONALLY IN A CELLCHIP-CONTAINING BIOREACTOR

ASAIO Journal ◽

10.1097/00002480-200503000-00003 ◽

2005 ◽

Vol 51 (2) ◽

pp. 1A

Author(s):

Eric Gottwald ◽

Caroline Augspurger ◽

Stefan Giselbrecht ◽

Alex Welle ◽

Karl-Friedrich Weibezahn

Keyword(s):

Cell Line ◽

Rat Hepatocytes ◽

Primary Rat Hepatocytes ◽

Hepatocyte Cell Line

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Pharmaceutical properties of 'sucupira' (Pterodon spp.)

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502010000400002 ◽

2010 ◽

Vol 46 (4) ◽

pp. 607-616 ◽

Cited By ~ 15

Author(s):

Daiane Hansen ◽

Mitsue Haraguchi ◽

Antonio Alonso

Keyword(s):

Biological Effects ◽

Chemical Compounds ◽

Experimental Models ◽

Popular Medicine ◽

Isolation And Characterization ◽

Pharmaceutical Properties ◽

The Relationship

The plant of the genus Pterodon (Fabaceae, Leguminosae), commonly known as 'sucupira' or 'faveira', are disseminated throughout the central region of Brazil and has frequently been used in popular medicine for its anti-rheumatic, analgesic, and anti-inflammatory properties. In recent years, interest in these plants has increased considerably. The biological effects of different phytoextracts and pure metabolites have been investigated in several experimental models in vivo and in vitro. The literature describes flavonoids, triterpene and steroids, while one paper presented studies with proteins isolated from the genus. This review provides an overview of phytochemical and pharmacological research in Pterodon, showing the main chemical compounds studied to date, and focusing on the relationship between these molecules and their biological activity. Furthermore, this study paves the way for more in-depth investigation, isolation and characterization of the molecules of this plant genus.

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