Identification, Isolation and Characterization of HCN4-Positive Pacemaking Cells Derived from Murine Embryonic Stem Cells during Cardiac Differentiation

2010 ◽  
Vol 33 (3) ◽  
pp. 290-303 ◽  
Author(s):  
KUMI MORIKAWA ◽  
UDIN BAHRUDIN ◽  
JUNICHIRO MIAKE ◽  
OSAMU IGAWA ◽  
YASUTAKA KURATA ◽  
...  
Cytokine ◽  
2009 ◽  
Vol 48 (1-2) ◽  
pp. 50-51
Author(s):  
Anne Schmitt ◽  
Andrea Küster ◽  
Rebekka Schneider ◽  
Martin Zenke ◽  
Valeria Poli ◽  
...  

Blood ◽  
2009 ◽  
Vol 114 (2) ◽  
pp. 268-278 ◽  
Author(s):  
Shannon L. McKinney-Freeman ◽  
Olaia Naveiras ◽  
Frank Yates ◽  
Sabine Loewer ◽  
Marsha Philitas ◽  
...  

Abstract Surface antigens on hematopoietic stem cells (HSCs) enable prospective isolation and characterization. Here, we compare the cell-surface phenotype of hematopoietic repopulating cells from murine yolk sac, aorta-gonad-mesonephros, placenta, fetal liver, and bone marrow with that of HSCs derived from the in vitro differentiation of murine embryonic stem cells (ESC-HSCs). Whereas c-Kit marks all HSC populations, CD41, CD45, CD34, and CD150 were developmentally regulated: the earliest embryonic HSCs express CD41 and CD34 and lack CD45 and CD150, whereas more mature HSCs lack CD41 and CD34 and express CD45 and CD150. ESC-HSCs express CD41 and CD150, lack CD34, and are heterogeneous for CD45. Finally, although CD48 was absent from all in vivo HSCs examined, ESC-HSCs were heterogeneous for the expression of this molecule. This unique phenotype signifies a developmentally immature population of cells with features of both primitive and mature HSC. The prospective fractionation of ESC-HSCs will facilitate studies of HSC maturation essential for normal functional engraftment in irradiated adults.


2011 ◽  
Vol 416 (1-2) ◽  
pp. 51-57 ◽  
Author(s):  
Min Young Lee ◽  
Esra Cagavi Bozkulak ◽  
Simon Schliffke ◽  
Peter J. Amos ◽  
Yongming Ren ◽  
...  

2011 ◽  
Vol 20 (11) ◽  
pp. 1817-1827 ◽  
Author(s):  
Maria K. Winzi ◽  
Poul Hyttel ◽  
Jacqueline Kim Dale ◽  
Palle Serup

2017 ◽  
Vol 21 ◽  
pp. 40-43
Author(s):  
Giulia Coppiello ◽  
Gloria Abizanda ◽  
Natalia Aguado ◽  
Elena Iglesias ◽  
Olalla Iglesias-Garcia ◽  
...  

2013 ◽  
Vol 32 (4) ◽  
pp. 789-800 ◽  
Author(s):  
Ying Cheng ◽  
Lu Wang ◽  
Ming Tang ◽  
Mengmeng Yin ◽  
Yurong Cui ◽  
...  

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