scholarly journals Random amplification of polymorphic DNA versus pulsed field gel electrophoresis of SmaI DNA macrorestriction fragments for typing strains of vancomycin-resistant enterococci

2000 ◽  
Vol 192 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Nicole Braak ◽  
Eddy Power ◽  
Richard Anthony ◽  
Hubert Ph. Endtz ◽  
Henri A. Verbrugh ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant Enterococci ◽  
Random Amplification ◽  
Vancomycin Resistant

scholarly journals Relapse and reacquisition of rectal colonization by vancomycin-resistantEnterococcus faeciumafter decolonization

2010 ◽  
Vol 138 (10) ◽  
pp. 1449-1453 ◽  
Author(s):  
W. G. LEE ◽  
I. J. PARK ◽  
H. Y. JIN ◽  
M. H. PARK
Keyword(s):  
Retrospective Study ◽  
Medical Records ◽  
Gel Electrophoresis ◽  
De Novo ◽  
Pulsed Field Gel Electrophoresis ◽  
Hospitalized Patients ◽  
Pulsed Field ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Rectal Colonization

SUMMARYTo better understand the epidemiology of colonization of vancomycin-resistant enterococci (VRE), we performed an 8-year retrospective study of all hospitalized patients with recurrent VRE colonization after they were documented as being clear of VRE and compared the primary colonization isolates and recolonization isolates by pulsed-field gel electrophoresis and Tn1546typing. Review of the medical records of all patients showed that of the 15 patients with recurrent colonization, six continued to be hospitalized on the same floor. Five were discharged home and then readmitted. Four were moved to another floor. Patients who remained on the same floor were recolonized with a strain that was indistinguishable from the original colonizing strain. Patients who were moved or were discharged hadde novoVRE colonization with strains distinct from the original colonizing strain.

scholarly journals Antimicrobial Resistance in Enterococci Isolated from Turkey Flocks Fed Virginiamycin

1998 ◽  
Vol 42 (3) ◽  
pp. 705-708 ◽  
Author(s):  
L. A. Welton ◽  
L. A. Thal ◽  
M. B. Perri ◽  
S. Donabedian ◽  
J. McMahon ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

ABSTRACT From 125 separate cloacal cultures from three turkey flocks fed virginiamycin, 104 Enterococcus faecium and 186Enterococcus faecalis isolates were obtained. As the turkeys aged, there was a higher percentage of quinupristin-dalfopristin-resistant E. faecium isolates, with isolates from the oldest flock being 100% resistant. There were no vancomycin-resistant enterococci. Results of pulsed-field gel electrophoresis (PFGE) indicated there were 11 PFGE types of E. faecalis and 7 PFGE types of E. faecium that were in more than one group of flock cultures.

scholarly journals Improved Pulsed-Field Gel Electrophoresis for Typing Vancomycin-Resistant Enterococci

2000 ◽  
Vol 38 (11) ◽  
pp. 4242-4245 ◽  
Author(s):  
Durmishkhan Turabelidze ◽  
Mamuka Kotetishvili ◽  
Arnold Kreger ◽  
J. Glenn Morris ◽  
Alexander Sulakvelidze
Keyword(s):  
Gel Electrophoresis ◽  
Cost Effective ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

A rapid protocol for subtyping vancomycin-resistant enterococci by pulsed-field gel electrophoresis is reported. The procedure is simple and potentially cost-effective and allows reproducible subtyping of the strains in approximately 1 day.

scholarly journals Vancomycin-Resistant Enterococcus faecium: Catheter Colonization, esp Gene, and Decreased Susceptibility to Antibiotics in Biofilm

2005 ◽  
Vol 49 (12) ◽  
pp. 5046-5050 ◽  
Author(s):  
Issam I. Raad ◽  
Hend A. Hanna ◽  
Maha Boktour ◽  
Gassan Chaiban ◽  
Ray Y. Hachem ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Antibiotic Susceptibility ◽  
Pulsed Field Gel Electrophoresis ◽  
Molecular Characteristics ◽  
Vancomycin Resistant Enterococcus ◽  
Catheter Colonization ◽  
Pulsed Field ◽  
Vancomycin Resistant ◽  
Gastrointestinal Colonization

ABSTRACT To evaluate the molecular characteristics and antibiotic susceptibility in biofilm of vancomycin-resistant Enterococcus faecium (VREF) organisms that had caused catheter-related VREF bacteremia (VREF-CRB), we compared 22 isolates causing bacteremia obtained from patients with VREF-CRB with 30 isolates from control patients with gastrointestinal colonization by VREF. Using pulsed-field gel electrophoresis, we identified 17 unique strains among the 22 VREF-CRB isolates and 23 strains among the gastrointestinal isolates. The esp gene was detected in 53% (9 of 17) of the VREF-CRB and 61% (14 of 23) of the control strains (P = 0.6). VREF-CRB produced heavier biofilm colonization of silicone disks than did control organisms (P < 0.001). Daptomycin, minocycline, and quinupristin-dalfopristin were each independently more active than linezolid in reducing biofilm colonization by VREF-CRB (P < 0.01), with daptomycin being the most active, followed by minocycline. In conclusion, the esp gene in VREF is not associated with heavy biofilm colonization or catheter-related bacteremia. In biofilm, daptomycin and minocycline were the most active antibiotics against VREF, and linezolid was the least active.

scholarly journals Molecular Characterization of Glycopeptide-Resistant Enterococcus faecium Isolates from Portuguese Hospitals

2005 ◽  
Vol 49 (7) ◽  
pp. 3073-3079 ◽  
Author(s):  
Carla Novais ◽  
João C. Sousa ◽  
Teresa M. Coque ◽  
Luísa V. Peixe
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant ◽  
Geographical Locations

ABSTRACT Fifty-one pulsed-field gel electrophoresis types and 17 Tn1546 variants were identified among 101 Enterococcus faecium isolates recovered in three distant Portuguese hospitals. Intra- and interhospital dissemination of specific strains and Tn1546 types was detected, which might largely contribute to the endemicity of vancomycin-resistant E. faecium in Portuguese hospitals, as happened previously in other geographical locations.

scholarly journals Local Genetic Patterns within a Vancomycin-Resistant Enterococcus faecalis Clone Isolated in Three Hospitals in Portugal

2004 ◽  
Vol 48 (9) ◽  
pp. 3613-3617 ◽  
Author(s):  
Carla Novais ◽  
Teresa M. Coque ◽  
João Carlos Sousa ◽  
Fernando Baquero ◽  
Luisa Peixe
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Vancomycin Resistant Enterococcus ◽  
Pulsed Field ◽  
Genetic Patterns ◽  
Single Clone ◽  
Vancomycin Resistant

ABSTRACT Eight pulsed-field gel electrophoresis subtypes and six Tn1546 variants were identified among Enterococcus faecalis isolates of a single clone recovered in three geographically separate Portuguese hospitals. Some clonal subtypes were found in particular hospitals, and Tn1546 variants were either widespread or confined to some of them. We also report on the first Tn1546 transposon containing an ISEf1 insertion.

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