Expression of the mast cell growth factor interleukin-3 in melanocytic lesions correlates with an increased number of mast cells in the perilesional stroma: implications for melanoma progression

Proliferation of murine mast cells is induced by both T-cell-derived and fibroblast-derived growth factors. Because the most potent T-cell- derived mast cell growth factor, interleukin-3, promotes the migration of mast cells, we investigated whether fibroblast-derived growth factors had the chemoattractive activity as well. Conditioned medium (CM) of BALB/3T3 fibroblasts induced the migration of cultured mast cells (CMC) derived from normal (+/+) mice. BALB/3T3-CM contained the mast cell growth factor (MGF)/stem cell factor (SCF)/kit ligand (KL), which is the ligand for the receptor encoded by the W (c-kit) gene. CMC derived from the spleen of W/W mice lack the extracellular domain of the W (c-kit) receptor, and W/W CMC did not proliferate in response to BALB/3T3-CM. However, W/W CMC did migrate normally toward BALB/3T3-CM and, moreover, the antibody to the extracellular domain of the W (c- kit) receptor did not inhibit the chemoattractive activity of +/+ CMC toward BALB/3T3-CM. These results indicated that MGF/SCF/KL itself did not represent the major chemoattractive activity. On the other hand, BALB/3T3-CM induced neither proliferation nor migration of CMC derived from mi/mi mice. Both W/W and mi/mi mice are deficient in mast cells, but the present results suggest that the mechanism of the abnormality is different between W/W and mi/mi mice.

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Topical tretinoin increases dermal mast cells, induces epidermal mast cell growth factor (c-kit ligand) and modulates its distribution in hairless mice

Archives of Dermatological Research ◽

10.1007/bf02505251 ◽

1996 ◽

Vol 288 (9) ◽

pp. 537-542 ◽

Cited By ~ 2

Author(s):

Lorraine H. Kligman ◽

George F. Murphy

Keyword(s):

Mast Cell ◽

Growth Factor ◽

Mast Cells ◽

Cell Growth ◽

Topical Tretinoin ◽

Hairless Mice ◽

Kit Ligand ◽

Factor C ◽

Mast Cell Growth Factor

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Characterization of a murine lymphokine distinct from interleukin 2 and interleukin 3 (IL-3) possessing a T-cell growth factor activity and a mast-cell growth factor activity that synergizes with IL-3.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.83.6.1857 ◽

1986 ◽

Vol 83 (6) ◽

pp. 1857-1861 ◽

Cited By ~ 79

Author(s):

C. A. Smith ◽

D. M. Rennick

Keyword(s):

Mast Cell ◽

T Cell ◽

Growth Factor ◽

Cell Growth ◽

Interleukin 2 ◽

Factor Activity ◽

Interleukin 3 ◽

Mast Cell Growth Factor ◽

T Cell Growth Factor

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BALB/3T3 fibroblast-conditioned medium attracts cultured mast cells derived from W/W but not from mi/mi mutant mice, both of which are deficient in mast cells

Blood ◽

10.1182/blood.v80.8.1933.1933 ◽

1992 ◽

Vol 80 (8) ◽

pp. 1933-1939 ◽

Cited By ~ 14

Author(s):

T Jippo-Kanemoto ◽

S Adachi ◽

Y Ebi ◽

H Matsuda ◽

T Kasugai ◽

...

Keyword(s):

Mast Cell ◽

Growth Factors ◽

T Cell ◽

Growth Factor ◽

Mast Cells ◽

Cell Growth ◽

Conditioned Medium ◽

Extracellular Domain ◽

Kit Receptor ◽

Mast Cell Growth Factor

Abstract Proliferation of murine mast cells is induced by both T-cell-derived and fibroblast-derived growth factors. Because the most potent T-cell- derived mast cell growth factor, interleukin-3, promotes the migration of mast cells, we investigated whether fibroblast-derived growth factors had the chemoattractive activity as well. Conditioned medium (CM) of BALB/3T3 fibroblasts induced the migration of cultured mast cells (CMC) derived from normal (+/+) mice. BALB/3T3-CM contained the mast cell growth factor (MGF)/stem cell factor (SCF)/kit ligand (KL), which is the ligand for the receptor encoded by the W (c-kit) gene. CMC derived from the spleen of W/W mice lack the extracellular domain of the W (c-kit) receptor, and W/W CMC did not proliferate in response to BALB/3T3-CM. However, W/W CMC did migrate normally toward BALB/3T3-CM and, moreover, the antibody to the extracellular domain of the W (c- kit) receptor did not inhibit the chemoattractive activity of +/+ CMC toward BALB/3T3-CM. These results indicated that MGF/SCF/KL itself did not represent the major chemoattractive activity. On the other hand, BALB/3T3-CM induced neither proliferation nor migration of CMC derived from mi/mi mice. Both W/W and mi/mi mice are deficient in mast cells, but the present results suggest that the mechanism of the abnormality is different between W/W and mi/mi mice.

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