The Penetration of Quinine, Salicylic Acid, PAS, Salicyluric Acid, Barbital and Lithium across the Vitreous Barrier of the Rabbit Eye

Abstract We have developed a specific and sensitive method for the determination of salicylic acid, salicyluric acid, and gentisic acid in urine. Any proteins present are precipitated with methyl cyanide. After centrifugation, an aliquot of the supernate is directly injected into an octadecyl silane reversed-phase chromatographic column, then eluted with a mixture of water, butanol, acetic acid, and sodium sulfate, and quantitated at 313 nm by ultraviolet detection according to peak-height ratios (with internal standard, o-methoxybenzoic acid) or peak heights (no internal standard). The method allows estimates within 25 min. Sensitivity was 0.2 mg/L for gentisic acid, and 0.5 mg/L for both salicyluric and salicylic acid (20-micro L injection volume); response was linear with concentration to at least 2.000 g/L for salicylic acid and metabolites. Analytical recovery of salicylic acid and metabolites from urine is complete. Intra-assay precision (coefficient of variation) is 5.52% at 7.5 mg/L for salicylic acid, 5.01% at 9.33 mg/L for salicyluric acid, and 3.07% at 7.96 mg/L for gentisic acid. Interassay precision is 7.32% at 7.51 mg/L for salicylic acid, 5.52% at 8.58 mg/L for salicyluric acid, and 3.97% at 8.32 mg/L for gentisic acid. We saw no significant interference in urine from patients being treated with various drugs other than aspirin.

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Renal Contribution to Overall Metabolism of Drugs II: Biotransformation of Salicylic Acid to Salicyluric Acid

Journal of Pharmaceutical Sciences ◽

10.1002/jps.2600610823 ◽

1972 ◽

Vol 61 (8) ◽

pp. 1284-1287 ◽

Cited By ~ 23

Author(s):

Suk Han Wan ◽

Sidney Riegelman

Keyword(s):

Salicylic Acid ◽

Salicyluric Acid

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Simultaneous liquid-chromatographic quantitation of salicylic acid, salicyluric acid, and gentisic acid in plasma.

Clinical Chemistry ◽

10.1093/clinchem/25.8.1420 ◽

1979 ◽

Vol 25 (8) ◽

pp. 1420-1425 ◽

Cited By ~ 47

Author(s):

B E Cham ◽

D Johns ◽

F Bochner ◽

D M Imhoff ◽

M Rowland

Keyword(s):

Salicylic Acid ◽

Gentisic Acid ◽

Salicyluric Acid ◽

Liquid Chromatographic

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Review for "Low levels of salicylic acid and salicyluric acid are present in synovial fluid of patients taking aspirin at the time of knee arthroplasty surgery"

10.1111/1440-1681.13333/v1/review1 ◽

2020 ◽

Keyword(s):

Salicylic Acid ◽

Synovial Fluid ◽

Knee Arthroplasty ◽

Salicyluric Acid ◽

Low Levels ◽

Knee Arthroplasty Surgery ◽

Arthroplasty Surgery

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Sustained blood concentrations of salicylic acid following rectal administration of salicyluric acid in dogs.

Chemical and Pharmaceutical Bulletin ◽

10.1248/cpb.37.2537 ◽

1989 ◽

Vol 37 (9) ◽

pp. 2537-2538 ◽

Cited By ~ 5

Author(s):

Junzo NAKAMURA ◽

Hideo SHIOTA ◽

Hitoshi SASAKI ◽

Juichiro SHIBASAKI

Keyword(s):

Salicylic Acid ◽

Rectal Administration ◽

Blood Concentrations ◽

Salicyluric Acid

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Metabolism of Aspirin after Therapeutic and Toxic Doses

Human & Experimental Toxicology ◽

10.1177/096032719000900302 ◽

1990 ◽

Vol 9 (3) ◽

pp. 131-136 ◽

Cited By ~ 23

Author(s):

D.K. Patel ◽

A. Hesse ◽

A. Ogunbona ◽

L.J. Notarianni ◽

P.N. Bennett

Keyword(s):

Salicylic Acid ◽

Therapeutic Dose ◽

Urinary Metabolites ◽

Urinary Recovery ◽

Main Metabolite ◽

Gentisic Acid ◽

Salicyluric Acid ◽

Glycine Conjugate ◽

Kinetics Of

1 The urinary recovery of metabolites of aspirin (ASA) was studied in 45 volunteers who took a therapeutic dose (600 mg) of ASA by mouth and in 37 patients who took ASA in overdose. 2 The main metabolite recovered from the volunteers was the glycine conjugate, salicyluric acid (SUA), which accounted for 75.01 ± 1.19% of total urinary metabolites, whereas salicylic acid (SA) accounted for 8.82 ± 0.56%. Recovery of SUA was negatively correlated with that of SA (r = -0.8625, P < 0.001). 3 In 24 patients with admission plasma salicylate concentrations of 240-360 mg 1-1, SUA accounted for 46.66 ± 3.22% and SA for 31.88 ± 4.02%. 4 In 13 patients with admission plasma salicylate concentrations of 715-870 mg 1-1, SUA accounted for 21.57 ± 3.65% and SA for 64.72 ± 4.82%. 5 Reduced excretion of salicylate as SUA was also accompanied by increased elimination as gentisic acid and salicylic acid phenolic glucuronide indicating that the unsaturated processes that lead to the formation of these metabolites contribute significantly (22-23%) to the inactivation of large doses of salicylate. 6 While the Michalis-Menten kinetics of ASA have been well demonstrated at lower doses, our findings illustrate the progressive saturation of SUA formation under conditions of increasing ASA load to toxic amounts and raise issues about the in-vivo glycine pool when ASA is taken in overdose.

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