DETECTION AND CROSS-REACTION OF H-Y ANTIGEN BY HAEMAGGLUTINATION

1978 ◽  
Vol 5 (5) ◽  
pp. 303-312 ◽  
Author(s):  
A. Shalev ◽  
I. Berczi ◽  
J. L. Hamerton
Keyword(s):  
Cross Reaction

Pharmacodynamics of [D-Ser (t-Bu), Des-Gly]GnRH ethylamide (Buserelin®) in 6 normal volunteers.

1987 ◽  
Vol 115 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Y. Reznik ◽  
B. P. Winiger ◽  
M. L. Aubert ◽  
P. C. Sizonenko
Keyword(s):  
Urinary Excretion ◽  
Precocious Puberty ◽  
Plasma Levels ◽  
Intranasal Administration ◽  
Cross Reaction ◽  
Disappearance Rate ◽  
Gnrh Analog ◽  
Male Adolescents ◽  
Normal Volunteers ◽  
The Mean

Abstract. The disappearance rate of [D-Ser(t-bu)6,des-Gly10]GnRH ethylamide (Buserelin®, HOE 766) was studied in plasma and urine after intranasal (300 μg) or sc (10 μg/kg) administration. A radioimmunoassay for HOE 766 was developed using 125I[D-Trp6,Des-Gly10]GnRH ethylamide as tracer and an antiserum raised against HOE 766. Cross-reaction with native GnRH was only 1.7%. Sensitivity was 1 pg/tube. In 6 male adolescents, the mean plasma HOE 766 concentration (± sem) was 0.46 ± 0.08, 0.50 ± 0.10, 0.28 ± 0.04, 0.24 ± 0.04, 0.13 ± 0.03, and 0.08 ± 0.02 μg/l 30, 60, 90, 120 and 180 min after the intranasal administration, respectively. Concomitant urinary excretion of HOE 766-like material was 9.43 ± 1.96 μg/4 h. There was a good correlation between integrated plasma levels and urinary excretion (r = 0.92). In the same 6 volunteers, the plasma HOE 766 levels were 21.2 ± 3.0, 25.9 ± 0.8, 21.2 ± 0.9, 17.1 ± 0.7, 12.8 ± 1.1, 8.9 ± 0.4, and 5.9 ± 0.8 μg/l 20, 40, 60, 90, 120, 180 and 240 min after sc injection, respectively. The mean urinary excretion was 543 ± 61 μg/4 h. In two girls with precocious puberty treated during 12 to 15 months with intranasal administration of HOE 766, urinary excretion of HOE 766-like material was shown to correlate well with the degree of inhibition of plasma 17β-E2and of plasma LH and FSH responses to a GnRH challenge. Thus, monitoring of HOE 766 in urine appears to be helpful for evaluating of intranasal therapy with a GnRH analog in precocious puberty.

scholarly journals Genuine and apparent cross-reaction of polyclonal antibodies to proteins and peptides

1994 ◽  
Vol 219 (1-2) ◽  
pp. 73-81 ◽  
Author(s):  
Lukas LEDER ◽  
Hans WENDT ◽  
Christian SCHWAB ◽  
Ilian JELESAROV ◽  
Susanne BORNHAUSER ◽  
...  
Keyword(s):  
Polyclonal Antibodies ◽  
Cross Reaction ◽  
Proteins And Peptides

scholarly journals The immunological characterization of several human ribonucleases by using primary binding tests

1977 ◽  
Vol 163 (3) ◽  
pp. 419-426 ◽  
Author(s):  
E A Neuwelt ◽  
M Schmukler ◽  
M S Niziak ◽  
P B Jewett ◽  
C C Levy
Keyword(s):  
Human Plasma ◽  
Cross Reaction ◽  
The Other ◽  
Human Pancreas ◽  
Human Tissues ◽  
Antigenic Difference ◽  
Immunological Characterization ◽  
Similarities And Differences

RNAases (ribonucleases), purified from four human tissues, as well as bovine pancreatic RNAase (RNAase A), were studied by immunodiffusion methods and by two different primary binding tests. The enzymes fell into two groups immunologically, those purified from plasma and pancreas in one and those from spleen and liver in the other. No antigenic cross-reaction between the two groups was detected by any of the immunoassays used. There was a slight antigenic cross-reaction between the human and bovine pancreatic RNAases. The liver and spleen RNAases were immunologically identical by all criteria used, whereas a small but consistent antigenic difference between the human plasma and human pancreas enzymes was detected. The significance of this difference between the human plasma and pancreas RNAases is discussed in relation to similarities and differences in their properties.

Absence of cross-reaction between HLA B27 and yersinia enterocolitica or chlamydia trachomatis in reactive arthritis and ankylosing spondylitis

1985 ◽  
Vol 4 (4) ◽  
pp. 487-487 ◽  
Author(s):  
J. Roudier ◽  
H. De Montclos ◽  
D. Thouvenot ◽  
J. J. Chomel ◽  
F. N. Guillermet ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
Chlamydia Trachomatis ◽  
Yersinia Enterocolitica ◽  
Reactive Arthritis ◽  
Cross Reaction ◽  
Hla B27

scholarly journals Streptococcus suis serotyping by a new multiplex PCR

2014 ◽  
Vol 63 (6) ◽  
pp. 824-830 ◽  
Author(s):  
Anusak Kerdsin ◽  
Yukihiro Akeda ◽  
Rujirat Hatrongjit ◽  
Unchaya Detchawna ◽  
Tsutomu Sekizaki ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Bacterial Species ◽  
Streptococcus Suis ◽  
Cross Reaction ◽  
The Third ◽  
Dna Mixture

A multiplex PCR was developed to detect all true serotypes of Streptococcus suis. This multiplex PCR was composed of four reaction sets. The first set identified nine serotypes (serotypes 1/2, 1, 2, 3, 7, 9, 11, 14 and 16), the second set identified eight serotypes (serotypes 4, 5, 8, 12, 18, 19, 24 and 25), the third set identified seven serotypes (serotypes 6, 10, 13, 15, 17, 23 and 31), and the last set identified five serotypes (serotypes 21, 27, 28, 29 and 30). This assay correctly detected serotypes 2, 5, 14 and 24 in human isolates, and serotypes 1, 2, 1/2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 15, 16, 17, 19, 24, 28 and 31 in pig isolates from Thailand. No cross-reaction was observed with other bacterial species. Our multiplex PCR was able to simultaneously amplify a DNA mixture of reference Streptococcus suis serotypes. This assay should be useful for serotype surveillance of human and pig isolates of Streptococcus suis.

scholarly journals Do intestinal parasites interfere with the seroepidemiologic surveillance of Schistosoma mansoni infection?

1996 ◽  
Vol 116 (3) ◽  
pp. 323-329 ◽  
Author(s):  
B. Alarcón De Noya ◽  
C. Colmenares ◽  
S. Losada ◽  
Z. Fermin ◽  
G. Masroua ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
False Positive ◽  
Total Population ◽  
Intestinal Parasites ◽  
Field Work ◽  
Cross Reactivity ◽  
Cross Reaction ◽  
Protein Fractions ◽  
Egg Antigen ◽  
Immunoenzymatic Assay

SUMMARYIn view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15·3% of the total population as determined by SEA–ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.

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