Direct plant regeneration from leaf explants of Plumbago species and its genetic fidelity through RAPD markers

2002 ◽  
Vol 140 (3) ◽  
pp. 305-313 ◽  
Author(s):  
G R ROUT
Keyword(s):  
Plant Regeneration ◽  
Rapd Markers ◽  
Leaf Explants ◽  
Genetic Fidelity ◽  
Direct Plant Regeneration

Direct plant regeneration from mature leaf explants of pistachio, Pistacia vera L.

2009 ◽  
Vol 121 (3) ◽  
pp. 361-365 ◽  
Author(s):  
Engin Tilkat ◽  
Ahmet Onay ◽  
Hakan Yıldırım ◽  
Emine Ayaz
Keyword(s):  
Plant Regeneration ◽  
Leaf Explants ◽  
Mature Leaf ◽  
Pistacia Vera ◽  
Direct Plant Regeneration

Direct plant regeneration from leaf explants of Drosera rotundifolia cultured in vitro

1995 ◽  
Vol 43 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Milan Bobák ◽  
Alžbeta Blehová ◽  
Jozef Krištín ◽  
Miroslav Ovečka ◽  
Jozef Šamaj
Keyword(s):  
Plant Regeneration ◽  
Leaf Explants ◽  
Drosera Rotundifolia ◽  
Direct Plant Regeneration

scholarly journals Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Temperature Treatment ◽  
In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

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