BIOCHEMICAL STUDIES ON BLACK GRAM (PHASEOLUS MUNGO): I. Solubilization and Electrophoretic Characterization of The Proteins.

1977 ◽  
Vol 1 (2) ◽  
pp. 111-129 ◽  
Author(s):  
V. W. PADHYE ◽  
D. K. SALUNKHE
Keyword(s):  
Black Gram ◽  
Phaseolus Mungo ◽  
Biochemical Studies

Isolation and Partial Characterization of Black Gram (Phaseolus mungo L) Starch

1982 ◽  
Vol 47 (5) ◽  
pp. 1524-1538 ◽  
Author(s):  
S. K. SATHE ◽  
P. D. RANGNEKAR ◽  
S. S. DESHPANDE ◽  
D. K. SALUNKHE
Keyword(s):  
Partial Characterization ◽  
Black Gram ◽  
Phaseolus Mungo

Studies of Crop Loss following Insect Attack on Cotton in East Africa

1957 ◽  
Vol 48 (4) ◽  
pp. 833-849 ◽  
Author(s):  
K. S. McKinlay ◽  
Q. A. Geering
Keyword(s):  
East Africa ◽  
Heliothis Armigera ◽  
Research Station ◽  
Crop Loss ◽  
Black Gram ◽  
Elephant Grass ◽  
Phaseolus Mungo ◽  
Maximum Loss ◽  
Perennial Legume ◽  
Insect Attack

The loss of crop following insect attack on cotton was studied in 1950–54 at the Cotton Research Station, Namulonge, which is situated in the elephant-grass zone of Uganda, in which the principal pest of cotton was originally considered to be Lygus vosseleri Popp. In 1953, the main invasion of cotton by Lygus occurred during September and October, originating from cultivated crops of black gram (Phaseolus mungo) and sorghum and from the wild perennial legume, Pseudarthria sp., which earlier workers had considered important. Trials in this zone have demonstrated, over a number of years, that early June is the optimum date for sowing cotton, although such sowings receive the heaviest attack by L. vosseleri. Experiments to determine the maximum loss of yield due to Lygus should therefore be made with cotton sown at this time. Bollworms are also important pests, notably Heliothis armigera (Hb.) and Earias spp., their attacks following those of Lygus and being heaviest in October–November.

scholarly journals Purification and characterization of acid phosphatase from a germinating black gram (Vigna mungo L.) seedling

2011 ◽  
Vol 63 (3) ◽  
pp. 747-756 ◽  
Author(s):  
A.K.M. Asaduzzaman ◽  
Habibur Rahman ◽  
Tanzima Yeasmin
Keyword(s):  
Acid Phosphatase ◽  
Gel Electrophoresis ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Inhibitory Effect ◽  
Maximum Activity ◽  
Exchange Chromatography ◽  
Black Gram ◽  
Km Value

An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange chromatography on DEAE cellulose. The acid phosphatase gave a single band on SDS-polyacrylamide slab gel electrophoresis. The molecular weight of the acid phosphatase determined by SDS-polyacrylamide slab gel electrophoresis was estimated to be 25 kDa. The purified enzyme showed maximum activity at pH 5 and at temperature of 55?C. Mg2+, Zn2+ and EDTA had an inhibitory effect on the activity of the acid phosphatase. Black gram seedling acid phosphatase was activated by K+, Cu2+ and Ba2+. The Km value of the enzyme was found to be 0.49 mM for pNPP as substrate.

Sign in / Sign up

Export Citation Format

Share Document