Different effects of pH on microbial biomass carbon and metabolic quotients by fumigation–extraction and substrate-induced respiration methods in soils under different climatic conditions

Selecting rhizosphere soil as the object of study which from 5 different kinds of plants in the Inner Mongolia University campus, we use fumigation extraction method and liquid chlorine fumigation extraction-water bath method to determine the microbial biomass carbon in the rhizosphere soil of different plants, in order to compare that whether or not liquid chlorine fumigation extraction-water bath method applicable to this type of soil sample. The results show that, there are significant differences in the rhizosphere soil microbial biomass carbon of 5 different kinds of plants, determination results is that Pyrus sorotina (LS) > Platycladus orientalis (CB) > Prunus persica (TS)> Syringa vulgaris (DX) > Pinus tabuliformis (YS), the content of P. sorotina is highest, 260.53 mg/kg, and P. tabulaeformis is lowest, 121.11 mg/kg, the differ is 139.42 mg/kg. There is a big gap between the measure values of the two methods, so that liquid chlorine fumigation extraction-water bath method doesn’t apply to the determination of this type microbial biomass carbon.

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Modification of the original chloroform fumigation extraction technique to allow measurement of δ13C of soil microbial biomass carbon

Soil Biology and Biochemistry ◽

10.1016/j.soilbio.2007.01.026 ◽

2007 ◽

Vol 39 (7) ◽

pp. 1724-1729 ◽

Cited By ~ 22

Author(s):

Evah W. Murage ◽

Paul R. Voroney

Keyword(s):

Microbial Biomass ◽

Soil Microbial Biomass ◽

Microbial Biomass Carbon ◽

Extraction Technique ◽

Biomass Carbon ◽

Soil Microbial ◽

Soil Microbial Biomass Carbon ◽

Chloroform Fumigation ◽

Fumigation Extraction

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Effect of liming on microbial biomass carbon of acidic arenosols in pot experiments

Plant Soil and Environment ◽

10.17221/174/2009-pse ◽

2010 ◽

Vol 56 (No. 6) ◽

pp. 268-273 ◽

Cited By ~ 4

Author(s):

T. Filep ◽

T. Szili-Kovács

Keyword(s):

Organic Matter ◽

Microbial Biomass ◽

Microbial Biomass Carbon ◽

Soil Samples ◽

First Year ◽

Biomass Carbon ◽

Experimental Conditions ◽

Pot Experiments ◽

Liming Material ◽

Substrate Induced Respiration

In the paper we investigate the effect of liming on the microbial biomass carbon (MBC) in pot experiments during two vegetation periods. There was also another goal to get better understanding of the role of dissolved organic matter (DOM) and its quality on microbial processes. Pot experiments were carried out on two acidic soils. Liming material treatment was 0, 1, 2, 3 g CaCO<sub>3</sub> /kg soil (corresponding with 0, 1.4, 2.8, 4.1 t CaCO<sub>3</sub> /ha, respectively). On both soils, 3-3 soil samples were taken for two growing periods and the substrate-induced respiration (SIR), dissolved organic carbon and nitrogen (DOC and DON), and soil pH were determined from the soil samples. The SIR can be used to characterize the active biomass within the total microbial biomass. Liming was found to increase soil respiration and consequently MBC in the first year of the experiment, but at the maximum lime rate these values stagnated or declined in many cases on each soil. In the second year, the effects of treatments were much lower both on Kisvárda and on Nyírlugos soils. Under the given experimental conditions, when the DOC/DON ratio rose to above 30–40, disturbances appeared in N supplies to microorganisms. The N content of the easily mineralisable organic matter in the soil became so low that it inhibited the reproduction of the microorganisms.

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