Background: Cycline-dependent kinase inhibitors (CDKi); roscovitine and purvalanol, are promising anti-cancer drugs due to their strong anti-proliferative effectiveness due to activation of PA catabolism. Besides
transforming acetylated spermine and spermidine into spermidine and putrescine, respectively, polyamine
oxidase (PAO) also generates hydrogen peroxide in high concentrations as a by-product. PAO was assumed
as a pivotal key molecule during drug-induced apoptosis in cancer cells. Our aim is to reveal the role of
PAO action in CDKi-triggered apoptosis in Puma knock-out HCT116 colon cancer cells.
Methods: HCT116 wt and HCT116 Puma-/-
cells were treated with Roscovitine and Purvalanol and cell
viability and apoptosis were determined. Protein was isolated from treated and untreated cells and key
molecules of cell cycle control and polyamine pathways were investigated at translational level. Polyamine
content was determined by HPLC for all conditions. MDL-72527 was used as a PAO inhibitor and apoptotic
cell death was analysed.
Results: Roscovitine and purvalanol induced apoptosis and increased the cytotoxic responses in HCT116
wt and HCT116 Puma-/-
colon carcinoma cell lines by modulating CDK1, 4, cyclin-B1, D3. Both, CDKi
altered intrinsic apoptotic pathways in HCT116 wt. Whereas, drug-induced apoptosis occurred caspase-independent in Puma-/-
colon cancer cells. Roscovitine and purvalanol up-regulated polyamine catabolic
enzymes, whereas CDK inhibitors decreased the polyamine levels in HCT116 wt and HCT116 Puma-/-
colon
cancer cells. In addition, PAO inhibitor MDL72527 prevented drug-induced apoptosis.
Conclusion: PAO expression profile might be a critical target in CDK inhibitors-triggered apoptosis in
HCT116 colorectal cancer cells. Thus, MAPK signaling pathway relations with cell cycle and polyamine
catabolic pathway investigations are in progress.