scholarly journals Gestational Hypoxia Induces White Matter Damage in Neonatal Rats: A New Model of Periventricular Leukomalacia

2004 ◽  
Vol 14 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Olivier Baud ◽  
Jean-Luc Daire ◽  
Yvette Dalmaz ◽  
Romain H Fontaine ◽  
Richard C. Krueger ◽  
...  
Keyword(s):  
White Matter ◽  
Periventricular Leukomalacia ◽  
Neonatal Rats ◽  
White Matter Damage ◽  
New Model

scholarly journals The Neuroprotective Effects of Xenon on Neonatal Rats with White Matter Damage by Regulating Expression of microRNA-210 and HIF-1αThe Neuroprotective Effects of Xenon on Neonatal Rats with White Matter Damage by Regulating Expression of microRNA-210 and HIF-1α

2020 ◽  
Author(s):  
Xiangyun Yin ◽  
Jixiu Zhao ◽  
Jian Jiang ◽  
Hongmin Xi ◽  
Xianghong Li ◽  
...  
Keyword(s):  
White Matter ◽  
Expression Level ◽  
Neonatal Rats ◽  
Rt Pcr ◽  
White Matter Damage ◽  
Neuroprotective Effects ◽  
Brain White Matter ◽  
Group A ◽  
Group B ◽  
Brain Tissues

Abstract Background:Premature infant is a significant health care burden. White matter damage (WMD) is a leading cause of acute mortality and chronic morbidity in preterm. Xenon (Xe) intervention was given to the 3-day-old neonatal rats with brain white matter injury. By detecting the changes in the expression level of microRNA210 and hypoxia inducible factor 1α (HIF-1α) in brain tissue before and after xenon intervention, we can research the molecular basis and the mechanism of neuroprotective on effect of xenon on brain white matter damage in neonatal rats.Methods:Three-day-old SD rats were randomly divided into sham group(Group A, n=24), lipopolysaccharide(LPS)+hypoxia-ischemia(HI) group (Group B, n=24) and LPS+HI+Xe group ( n=72). The onset of Xe inhalation started at 0,2 and 5 hours in subgroups C,D,and E respectively.We investigated the neurobehavioral deficits by performing TUNEL and hematoxylin and eosin (HE) staining and examining the expression of miR-210and HIF-1α in brain tissues via RT-PCR and western blot. Results: Xe treatment improved the histological alterations and decreased the number of apoptotic cells in group C pups.Compared to group A,Detection of miR-210 level by RT-PCR. the expression level of miR-210 in neonatal rats' periventricular tissue increased significantly at all time points in group B (p<0.05).While the expression level of miR-210 in brain tissues of group B was significantly lower at 48h and 72h than that of group C(p<0.05).Similarly,Detection of HIF-1α protein by Western blot. The level of HIF-1α protein in group B brain tissues was significantly higher than that of group A at each time point (p<0.05), Xe treatment resulted in a marked increase in HIF-1α in C,D, and E subgroups (P < 0.05, compared to group B).Conclusions: These results demonstrate that the expression of HIF-1α and miR-210 increased in periventricular tissues and Xe could relieve the white matter damage by up-regulating the expression of HIF-1α and its target gene miR-210.The Xe therapeutic time window was within 5 hours after intervention, the sooner the better.

scholarly journals 8 Impact of Inhaled Nitric Oxide on Hyperoxia-Induced White Matter Damage in Neonatal Rats

2012 ◽  
Vol 97 (Suppl 2) ◽  
pp. A2-A3
Author(s):  
H. Pham ◽  
G. Vottier ◽  
J. Pansiot ◽  
B. Bollen ◽  
H. Kadar ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
White Matter ◽  
Inhaled Nitric Oxide ◽  
Neonatal Rats ◽  
White Matter Damage

A new model of white matter injury in neonatal rats with bilateral carotid artery occlusion

1999 ◽  
Vol 837 (1-2) ◽  
pp. 213-220 ◽  
Author(s):  
Hisakazu Uehara ◽  
Hiroshi Yoshioka ◽  
Shoji Kawase ◽  
Hideyuki Nagai ◽  
Tadaki Ohmae ◽  
...  
Keyword(s):  
White Matter ◽  
Carotid Artery ◽  
Artery Occlusion ◽  
Carotid Artery Occlusion ◽  
White Matter Injury ◽  
Neonatal Rats ◽  
New Model ◽  
Bilateral Carotid ◽  
Bilateral Carotid Artery Occlusion ◽  
Bilateral Carotid Artery

Inhaled NO prevents hyperoxia-induced white matter damage in neonatal rats

2014 ◽  
Vol 252 ◽  
pp. 114-123 ◽  
Author(s):  
Hoa Pham ◽  
Gaelle Vottier ◽  
Julien Pansiot ◽  
Sy Duong-Quy ◽  
Bieke Bollen ◽  
...  
Keyword(s):  
White Matter ◽  
Neonatal Rats ◽  
White Matter Damage ◽  
Inhaled No

Expression of ephrinB2 and EphB4 in a neonatal rat model of periventricular white matter damage

2015 ◽  
Vol 43 (3) ◽  
Author(s):  
Lihua Zhu ◽  
Lijuan Qian ◽  
Shiyu Wang ◽  
Ting Wang ◽  
Li Jiang
Keyword(s):  
White Matter ◽  
Rat Model ◽  
Vascular System ◽  
Periventricular Leukomalacia ◽  
Vascular Supply ◽  
Neonatal Rat ◽  
Cerebral White Matter ◽  
Periventricular White Matter ◽  
White Matter Damage ◽  
Hypoxia Ischemia

AbstractPeriventricular white matter damage (PWMD), also termed periventricular leukomalacia, is the predominant neurologic lesion in preterm infants. It appears to relate in part to the development of the vascular supply to the cerebral white matter. We investigated whether, in case of severe hypoxia-ischemia, the vascular system would be subject to severe damage or remodeled.To evaluate microvessel density (MVD) and the use of ephrinB2 and its receptor EphB4 to mark arterioles and venules to establish the correct anatomic assignment of the remodeled vessels in a hypoxia-induced PWMD rat model.Postnatal day 3 rats underwent permanent ligation of the right common carotid artery followed by 6% OCompared with sham rats, MVD, ephrinB2 and EphB4 levels were higher in the brains of hypoxic-ischemic rats. Similar percentages of vessels expressed ephrinB2 and EphB4 in sham rats, but expression of ephrinB2 was greater in brains injured by hypoxia-ischemia.Following hypoxic-ischemic injury to the rat brain, microvessels were remodeled and more arterioles than venules were acquired.

scholarly journals Impact of inhaled nitric oxide on white matter damage in growth-restricted neonatal rats

2015 ◽  
Vol 77 (4) ◽  
pp. 563-569 ◽  
Author(s):  
Hoa Pham ◽  
An Phan Duy ◽  
Julien Pansiot ◽  
Bieke Bollen ◽  
Jorge Gallego ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
White Matter ◽  
Inhaled Nitric Oxide ◽  
Neonatal Rats ◽  
White Matter Damage

scholarly journals Protective effects of melatonin on the white matter damage of neonatal rats by regulating NLRP3 inflammasome activity

Neuroreport ◽  
2021 ◽  
Vol 32 (9) ◽  
pp. 739-747
Author(s):  
Miao Qin ◽  
Yan Liu ◽  
Mengya Sun ◽  
Xianghong Li ◽  
Jiaxin Xu ◽  
...  
Keyword(s):  
White Matter ◽  
Nlrp3 Inflammasome ◽  
Protective Effects ◽  
Neonatal Rats ◽  
White Matter Damage ◽  
Inflammasome Activity

scholarly journals The neuroprotective effects of xenon on neonatal rats with white matter damage by regulating expression of microRNA-210 and HIF-1α

2019 ◽  
Author(s):  
xiangyun yin ◽  
Jixiu Zhao ◽  
Jian Jiang ◽  
Hongmin Xi ◽  
Xianghong Li ◽  
...  
Keyword(s):  
White Matter ◽  
Expression Level ◽  
Neonatal Rats ◽  
White Matter Damage ◽  
Neuroprotective Effects ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Time Point ◽  
Brain Tissues

Abstract Background: White matter damage is a leading cause of acute mortality and chronic morbidity in preterm birth. Xenon is a general anesthetic with neuroprotective effects. We aimed to reveal the molecular basis and neuroprotective mechanism of Xe intervention in treating white matter damage by detecting the expression level of miR-210 and HIF-1α in brain tissues of 3-day-old neonatal rats. Methods: Three-day-old SD rats were randomly divided into sham group (Group A, n=24), LPS +HI group (Group B, n=24) and LPS+HI+Xe group (n=72). LPS+HI+Xe group was given Xenon gas inhalation for three hours after treatment of HI at 0h,2h,and 5h,and divided into three subgroups C,D,and E randomly. We investigated the WMD by performing TUNEL and hematoxylin and eosin (HE) staining and examining the expression of miR-210and HIF-1α in brain tissues via RT-PCR and western blot. Results: Our study shows that the brain tissues of neonatal rat which stained by HE were pale, structure loosen, and psychosis along with apoptosis in group B, Xe treatment improved histological alterations and decreased the number of apoptotic cells in group C . The expression level of miR-210 increased significantly at all time points in group B compared to group A (p<0.05),While that was significantly lower at 0h and 48h than that of group C(p<0.05).Compared with group C,the expression of miR-210 in group D and group E decreased significantly at 0h, 24h and 48h (p<0.05).The level of HIF-1α protein in group B was significantly higher than that of group A at each time point, while significantly lower than that of group C at each time point and that of group D and E at 0h, 24h and 72h (p<0.05).Compared with group C and group D,the expression level of HIF-1α protein decreased significantly at 24h in group E (p<0.05). Conclusions: These results demonstrate that the expression of HIF-1α and miR-210 increased in periventricular tissues and Xe could relieve the white matter damage by up-regulating the expression of HIF-1α and its target gene miR-210.The Xe therapeutic time window was within 5 hours after intervention, the sooner the better. Keywords: premature birth, Xenon, microRNA-210, HIF-1α, white matter damage

scholarly journals Acute and Chronic Cerebral White Matter Damage in Neonatal Hydrocephalus

1994 ◽  
Vol 21 (4) ◽  
pp. 299-305 ◽  
Author(s):  
Marc R. Del Bigio ◽  
Marcia C. da Silva ◽  
James M. Drake ◽  
Ursula I. Tuor
Keyword(s):  
White Matter ◽  
Periventricular Leukomalacia ◽  
Cerebral White Matter ◽  
Periventricular White Matter ◽  
Histopathological Changes ◽  
Neonatal Brain ◽  
White Matter Damage ◽  
White Matter Changes ◽  
Deep Layers ◽  
Cat Model

Abstract:The neonatal cat model of kaolin-induced hydrocephalus is associated with progressive and severe ventriculomegaly. In this experiment we studied the evolution of the histopathological changes in hydrocephalic (n = 23) cats from 5–168 days after the induction of hydrocephalus along with age-matched controls (n = 10). In the periventricular white matter, extracellular edema and axonal damage were present within days of the onset of hydrocephalus. This was followed by reactive gliosis, white matter atrophy, and in some animals gross cavitation of the white matter. Even in the chronic, apparently compensated state there was ongoing glial cell death. Six cats were shunted an average of 23.6 ± 6.5 days after the induction of hydrocephalus because they were no longer able to feed independently. In spite of clinical improvement the white matter changes persisted. Overt cortical changes were minimal except where areas of white matter destruction encroached upon the deep layers. The white matter changes are very similar to those seen in periventricular leukomalacia and suggest that ischemia plays a role in neonatal brain injury caused by hydrocephalus.

Sign in / Sign up

Export Citation Format

Share Document