DIRECT SUPERVISION: ADDING COMPUTER-ASSISTED FEEDBACK AND DATA CAPTURE TO LIVE SUPERVISION

INTRODUCTIONTotal knee arthroplasty (TKA) is widely performed for improving pain and restoring function for patients with osteoarthritis (OA) and over 50000 primary procedures were performed in Australia in 2015 (Annual report 2016). Computer assisted pre-operative planning (CAPP) was introduced to enhance accuracy of rotational and translational alignment of components (Schep 2003). It can be done with 3D bone surface modelling from CT or MRI scans and take kinematics and surgeon work flow into account. Computer assisted intra-operative navigation is also accessed by some surgeons and consists of three elements: software platform, point positions from reference arrays captured and reference device attached to the patient’s bone (Bae 2011).By incorporating CAPP with navigation, a correlation between the patient’s anatomy from pre-operative images can be established by gathering intra-operative anatomical landmarks and/or surface points and is known as registration (Chan 2016). However, identifying landmarks and acquiring images intra-operatively and the registration quality can be time-consuming and/or prone to risk. Custom-designed registration software that could reduce the time and error and be incorporated into the surgical workflow is needed (Chan 2016).The aim of this study was to develop custom registration software using pre-operative planning and intra-operative 3D data capture and validate precision and repeatability using a preliminary Sawbones study.

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Multimedia Appendix Correction: A Computer-Assisted Personal Interview App in Research Electronic Data Capture for Administering Time Trade-off Surveys (REDCap): Development and Pretest (Preprint)

10.2196/preprints.11436 ◽

2018 ◽

Author(s):

Mark Oremus ◽

Anis Sharafoddini ◽

Gian Paolo Morgano ◽

Xuejing Jin ◽

Feng Xie

Keyword(s):

Data Capture ◽

Computer Assisted ◽

Electronic Data Capture ◽

Personal Interview ◽

Electronic Data ◽

Trade Off

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3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102237 ◽

1988 ◽

Vol 46 ◽

pp. 30-31

Author(s):

E. T. O'Toole ◽

R. R. Hantgan ◽

J. C. Lewis

Keyword(s):

Whole Blood ◽

Colloidal Gold ◽

Blood Platelets ◽

Cell Contact ◽

Computer Assisted ◽

Adherent Cells ◽

Differential Centrifugation ◽

Computer Reconstruction ◽

Sds Page ◽

Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

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Computer Assisted Image Reconstruction of Oligomer Structure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100092323 ◽

1976 ◽

Vol 34 ◽

pp. 472-473

Author(s):

A.M. Jones ◽

A. Max Fiskin

Keyword(s):

Three Dimensional ◽

Depth Of Field ◽

Computer Assisted ◽

Projection Data ◽

Two Dimensional ◽

Single Particles ◽

Dimensional Reconstruction ◽

Computer Assisted Image ◽

The Fourier Transform ◽

Space Approach

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.

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Biodegradable polyester neuroprostheses promote the reconnection of separated peripheral nerve stubs

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130109 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1094-1095

Author(s):

Beverly L. Giammara ◽

Jennifer S. Stevenson ◽

Peggy E. Yates ◽

Robert H. Gunderson ◽

Jacob S. Hanker

Keyword(s):

Image Analysis ◽

Basement Membrane ◽

Periodic Acid ◽

Light And Electron Microscopy ◽

Computer Assisted ◽

Type Iii Collagen ◽

Image Analysis System ◽

Biodegradable Polyester ◽

Adult Rats ◽

Type Iv

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.

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High-resolution measurement of birefringent fine structure in living cells using a new polarized light microscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100136647 ◽

1995 ◽

Vol 53 ◽

pp. 54-55

Author(s):

Rudolf Oldenbourg

Keyword(s):

Light Microscope ◽

Fluorescent Dyes ◽

Polarized Light ◽

Processing System ◽

Video Camera ◽

Molecular Organization ◽

Computer Assisted ◽

Image Recording ◽

Birefringent Crystal ◽

Technological Advances

The recent renaissance of the light microsope is fueled in part by technological advances in components on the periphery of the microscope, such as the laser as illumination source, electronic image recording (video), computer assisted image analysis and the biochemistry of fluorescent dyes for labeling specimens. After great progress in these peripheral parts, it seems timely to examine the optics itself and ask how progress in the periphery facilitates the use of new optical components and of new optical designs inside the microscope. Some results of this fruitful reflection are presented in this symposium.We have considered the polarized light microscope, and developed a design that replaces the traditional compensator, typically a birefringent crystal plate, with a precision universal compensator made of two liquid crystal variable retarders. A video camera and digital image processing system provide fast measurements of specimen anisotropy (retardance magnitude and azimuth) at ALL POINTS of the image forming the field of view. The images document fine structural and molecular organization within a thin optical section of the specimen.

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