Biodegradable polyester neuroprostheses promote the reconnection of separated peripheral nerve stubs

1992 ◽  
Vol 50 (2) ◽  
pp. 1094-1095
Author(s):  
Beverly L. Giammara ◽  
Jennifer S. Stevenson ◽  
Peggy E. Yates ◽  
Robert H. Gunderson ◽  
Jacob S. Hanker
Keyword(s):  
Image Analysis ◽  
Basement Membrane ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Computer Assisted ◽  
Type Iii Collagen ◽  
Image Analysis System ◽  
Biodegradable Polyester ◽  
Adult Rats ◽  
Type Iv

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.

scholarly journals Microcomputer-Based Quantification of Maize Streak Virus Symptoms in Zea mays

1998 ◽  
Vol 88 (5) ◽  
pp. 422-427 ◽  
Author(s):  
Darrin P. Martin ◽  
Edward P. Rybicki
Keyword(s):  
Zea Mays ◽  
Image Analysis ◽  
Maize Streak Virus ◽  
Computer Assisted ◽  
Streak Virus ◽  
Image Analysis System ◽  
Analysis Techniques ◽  
Computer Assisted Image ◽  
Analysis System ◽  
Image Analysis Techniques

We investigated the use of computer-assisted image analysis techniques for the objective quantification of maize streak virus (MSV) symptoms in Zea mays. We compared independent duplicate evaluations of chlorotic lesion areas occurring on MSV-infected leaves using visual assessment, a commercial image analysis system, and a custom image analysis system employing software developed in our laboratory. Relative to visual assessments of disease severity, computer-assisted image analysis employing both the commercial and custom systems provided significant enhancements in the accuracy and precision of chlorotic area estimations. The commercial image analysis system afforded no significant improvement in precision or accuracy over the custom system. An important advantage of examining images using the custom-written software was that the software permitted a high degree of analysis automation. Digitized images of maize leaves could be automatically analyzed by the custom software five times faster than, and with the same precision and accuracy as, when the same images were analyzed with the commercial software. Because of the flexibility of the image analysis techniques described, they should be applicable to the measurement of symptom severity in other plant host-pathogen combinations.

Immunohistochemical Analysis of Type III and IV Collagens in Tubulointerstitial Damage in Human Benign Nephrosclerosis

1995 ◽  
Vol 23 (6) ◽  
pp. 480-486 ◽  
Author(s):  
M S Razzaque ◽  
M Cheng ◽  
Y Horita ◽  
M Nishihara ◽  
T Harada ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Structural Changes ◽  
Immunohistochemical Analysis ◽  
Type Iii Collagen ◽  
Type Iii ◽  
Type Iv ◽  
Tubulointerstitial Damage ◽  
Basement Membrane Collagen ◽  
Immunohistochemical Techniques

Prolonged hypertension causes structural changes including glomerulosclerosis and tubulointerstitial damage of the kidney, termed benign nephrosclerosis. It is generally accepted that, in benign nephrosclerosis, increased accumulation of extracellular matrix in the glomeruli results in glomerulosclerosis. Little is known, however, about the possible role of the extracellular matrix in the tubulointerstitial damage in benign nephrosclerosis. In this study, the possible roles of type IV basement-membrane collagen and type III interstitial collagen in tubulointerstitial damage caused by hypertension were explored. Immunohistochemical techniques were used to investigate the distribution of type III and type IV collagens in the kidney sections of 15 patients with benign nephrosclerosis with tubulointerstitial damage and in 10 controls. In the control renal sections strong immunostaining for type III collagen was found in the interstitium and immunostaining for type IV collagen was present in the tubular basement membrane and weakly in the interstitium. In the patients with tubulointerstitial damage there was increased immunostaining for both type III and type IV collagens in the expanded interstitium and damaged tubules than was found in the control kidney sections. These findings indicate that increased accumulation of both type III and type IV collagens might play a significant role in the tubulointerstitial damage in benign nephrosclerosis.

Use of a Biodegradable Neuroprosthesis Containing Laminin for Avulsive Peripheral Nerve Injury Results in Early Endoneurial and Perineurial Collagen Type III Formation

1990 ◽  
Vol 48 (3) ◽  
pp. 158-159
Author(s):  
B. Giammara ◽  
E. Anderson ◽  
P. Yates ◽  
J. Zuniga ◽  
J. Hanker
Keyword(s):  
Basement Membrane ◽  
Peripheral Nerve ◽  
Collagen Type ◽  
Collagen Type Iv ◽  
Type Iii Collagen ◽  
Distal Stump ◽  
Type Iii ◽  
Type Iv ◽  
Direct Anastomosis ◽  
Leg Movement

The direct anastomosis of nerve stumps is most desirable after peripheral nerve transection. There are many avulsive nerve injuries, however, where a nerve segment is destroyed or must be removed -- and the proximal and distal stumps are separated by a gap requiring bridging. Earlier studies showed that a biodegradable polyester mesh (polyglactin 910, Vicryl™) sleeve can act as a conduit and guide regenerating axons into the distal stump across an 11 mm gap created in rat sciatic nerve. This is facilitated by filling the sleeve with Matrigel™, an extracellular basement membrane gel containing 80% laminin, smaller amounts of collagen type IV, heparan sulfate proteoglycan and entaction. The avulsed sciatic was reconnected in animals examined after 30 and 45 days and sensation and leg movement were much improved.The PATS variation of the PAS reaction can be used to demonstrate all glycomacromolecules containing alpha-diglycol groups. Although it also stains basement membrane or type IV collagen brown, it stains type III collagen or reticulin intensely black.

Alpha-, beta-MHC mRNA quantification in adult cardiomyocytes by in situhybridization: effect of thyroid hormone

1993 ◽  
Vol 265 (1) ◽  
pp. C62-C71 ◽  
Author(s):  
I. Dubus ◽  
A. Mercadier ◽  
O. Lucas ◽  
F. Contard ◽  
O. Nallet ◽  
...  
Keyword(s):  
Experimental Point ◽  
Computer Assisted ◽  
Image Analysis System ◽  
Mrna Levels ◽  
Dot Blot ◽  
Adult Rats ◽  
In Situhybridization ◽  
Analysis System

Cardiac myocytes isolated from adult rats and cultured for up to 5 days in a defined serum- and 3,5,3'-triiodothyronine-(T3) free medium were processed for in situ hybridization using [35S]cRNA probes specific for alpha- or beta-myosin heavy chain (MHC) mRNAs. A computer-assisted image analysis system was used to quantitate the hybridization signals within individual myocytes (100 cells/experimental point). The method was validated by comparison with dot-blot quantitation. The mean alpha-MHC mRNA density per cell decreased by 50% (P < 0.01) after 2 days in culture and remained stable thereafter, whereas the relative amount of beta-MHC mRNA did not increase until day 5. Addition of 10(-12) M T3 to the culture medium for 2 or 3 days was sufficient to maintain alpha-MHC mRNA levels similar to the day 0 values, whereas 10(-9) M T3 was necessary to completely inhibit beta-MHC mRNA expression. The independent analysis of myocytes exhibiting different morphological phenotypes with time in culture demonstrated that rounded myocytes contain relatively more alpha-MHC mRNA and were as sensitive to T3 as their rod-shaped counterparts. Their beta-MHC RNA content was similar to that found in rod-shaped cells and was still depressed by T3. In conclusion, we show that 1) physiological doses of T3 are sufficient to maintain in vitro a MHC phenotype close to that observed in vivo in adult, 2) the dose responsiveness of adult myocytes to T3 differs from that reported in neonatal myocytes, and 3) the alpha-MHC mRNA content and the T3 sensitivity of spheroidal myocytes imply that there is no alteration in their state of maturation.

A multi-resolution image analysis system for computer-assisted grading of neuroblastoma differentiation

2008 ◽  
Author(s):  
Jun Kong ◽  
Olcay Sertel ◽  
Hiroyuki Shimada ◽  
Kim L. Boyer ◽  
Joel H. Saltz ◽  
...  
Keyword(s):  
Image Analysis ◽  
Computer Assisted ◽  
Image Analysis System ◽  
Resolution Image ◽  
Analysis System

scholarly journals The Histochemical Alterations of Mucin in Colorectal Carcinoma Quantified by Two Efficient Algorithms of Digital Image Analysis

2019 ◽  
Vol 20 (18) ◽  
pp. 4580 ◽  
Author(s):  
Kasprzak ◽  
Seraszek-Jaros ◽  
Jagielska ◽  
Helak-Łapaj ◽  
Siodła ◽  
...  
Keyword(s):  
Image Analysis ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Periodic Acid ◽  
Daily Practice ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Computer Assisted ◽  
Periodic Acid Schiff ◽  
Tissue Samples

The practical use of knowledge on the diagnostic-prognostic role of polysaccharide components of mucins in colorectal cancer (CRC) has been difficult, due to the number of histochemical (HC) reaction types, as well as lack of standard methods of computer-assisted analysis of tissue expression of these molecules. Using two algorithms of digital image analysis (by application of Image-Pro Premier and our originally designed program Filter HSV), we evaluated the expression of polysaccharides in tissue samples of CRC patients (n = 33), and fragments of normal colorectal tissue from the same patients (control) using periodic acid Schiff reaction (PAS) (neutral mucins) and alcian blue staining (AB) (acidic mucins). Our results indicate lower expression of the PAS+ and AB+ mucins in CRC, as compared to the control samples. The higher expression of PAS+ polysaccharides was detected in flat tumors than in protruded CRC, while higher AB+ mucins expression was a feature of mucinous CRC subtypes. Positive correlation between mutual PAS+ and AB+ expression, as well as correlations with glucose concentration (PAS+ mucins), and hemoglobin level (AB+ mucins) were observed exclusively in unchanged colorectal samples (control). Both algorithms of digital image analysis (smart segmentation and Filter HSV) work properly and can be used interchangeably in daily practice of pathologists, as useful tools of quantitative evaluation of HC reaction in both normal and cancerous tissues.

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