scholarly journals Identification of eleven differentially expressed proteins in the tear film of patients with neovascular Age‐related Macular Degeneration (AMD)

2022 ◽  
Vol 100 (S267) ◽  
Author(s):  
Mateusz Winiarczyk ◽  
Dagmara Winiarczyk ◽  
Katarzyna Michalak ◽  
Kai Kaarniranta ◽  
Łukasz Adaszek ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Tear Film ◽  
Age Related Macular Degeneration ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Age Related

scholarly journals Dysregulated Tear Film Proteins in Macular Edema Due to the Neovascular Age-Related Macular Degeneration Are Involved in the Regulation of Protein Clearance, Inflammation, and Neovascularization

2021 ◽  
Vol 10 (14) ◽  
pp. 3060
Author(s):  
Mateusz Winiarczyk ◽  
Dagmara Winiarczyk ◽  
Katarzyna Michalak ◽  
Kai Kaarniranta ◽  
Łukasz Adaszek ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Macular Edema ◽  
Tear Film ◽  
Developed Countries ◽  
Age Related Macular Degeneration ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Neovascular Amd ◽  
Protein Clearance ◽  
Age Related

Macular edema and its further complications due to the leakage from the choroidal neovascularization in course of the age-related macular degeneration (AMD) is a leading cause of blindness among elderly individuals in developed countries. Changes in tear film proteomic composition have been reported to occur in various ophthalmic and systemic diseases. There is an evidence that the acute form of neovascular AMD may be reflected in the tear film composition. Tear film was collected with Schirmer strips from patients with neovascular AMD and sex- and age-matched control patients. Two-dimensional electrophoresis was performed followed by MALDI-TOF mass spectrometry for identification of differentially expressed proteins. Quantitative analysis of the differential electrophoretic spots was performed with Delta2D software. Altogether, 11 significantly differentially expressed proteins were identified; of those, 8 were downregulated, and 3 were upregulated in the tear film of neovascular AMD patients. The differentially expressed proteins identified in tear film were involved in signaling pathways associated with impaired protein clearance, persistent inflammation, and neovascularization. Tear film protein analysis is a novel way to screen AMD-related biomarkers.

scholarly journals Identification of aberrantly methylated differentially expressed genes in age-related macular degeneration

Medicine ◽  
2019 ◽  
Vol 98 (14) ◽  
pp. e15083
Author(s):  
Zixuan Xu ◽  
Zhaohui Ruan ◽  
Xuetao Huang ◽  
Qiang Liu ◽  
Zhaozhi Li ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Differentially Expressed Genes ◽  
Age Related Macular Degeneration ◽  
Differentially Expressed ◽  
Age Related

scholarly journals Ocular Surface Temperature in Age-Related Macular Degeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Andrea Sodi ◽  
Sara Matteoli ◽  
Giovanni Giacomelli ◽  
Lucia Finocchio ◽  
Andrea Corvi ◽  
...  
Keyword(s):  
Surface Temperature ◽  
Macular Degeneration ◽  
Ocular Surface ◽  
Tear Film ◽  
Ocular Blood Flow ◽  
Processing Technique ◽  
Age Related Macular Degeneration ◽  
Image Processing Technique ◽  
Control Group ◽  
Age Related

Background. The aim of this study is to investigate the ocular thermographic profiles in age-related macular degeneration (AMD) eyes and age-matched controls to detect possible hemodynamic abnormalities, which could be involved in the pathogenesis of the disease.Methods. 32 eyes with early AMD, 37 eyes with atrophic AMD, 30 eyes affected by untreated neovascular AMD, and 43 eyes with fibrotic AMD were included. The control group consisted of 44 healthy eyes. Exclusion criteria were represented by any other ocular diseases other than AMD, tear film abnormalities, systemic cardiovascular abnormalities, diabetes mellitus, and a body temperature higher than 37.5°C. A total of 186 eyes without pupil dilation were investigated by infrared thermography (FLIR A320). The ocular surface temperature (OST) of three ocular points was calculated by means of an image processing technique from the infrared images. Two-samplet-test and one-way analysis of variance (ANOVA) test were used for statistical analyses.Results. ANOVA analyses showed no significant differences among AMD groups (Pvalue >0.272). OST in AMD patients was significantly lower than in controls (P>0.05).Conclusions. Considering the possible relationship between ocular blood flow and OST, these findings might support the central role of ischemia in the pathogenesis of AMD.

scholarly journals Tear film proteome in age-related macular degeneration

2018 ◽  
Vol 256 (6) ◽  
pp. 1127-1139 ◽  
Author(s):  
Mateusz Winiarczyk ◽  
Kai Kaarniranta ◽  
Stanisław Winiarczyk ◽  
Łukasz Adaszek ◽  
Dagmara Winiarczyk ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Tear Film ◽  
Age Related Macular Degeneration ◽  
Age Related

scholarly journals Integrated bioinformatics analysis of aberrantly-methylated differentially-expressed genes and pathways in age-related macular degeneration

2020 ◽  
Author(s):  
Yinchen Shen ◽  
Mo Li ◽  
Kun Liu ◽  
Xiaoyin Xu ◽  
Shaopin Zhu ◽  
...  
Keyword(s):  
Network Analysis ◽  
Macular Degeneration ◽  
Differentially Expressed Genes ◽  
Bioinformatics Analysis ◽  
Age Related Macular Degeneration ◽  
Differentially Expressed ◽  
Ppi Network ◽  
Hub Genes ◽  
Age Related ◽  
Low Expression

Abstract Background: Age-related macular degeneration (AMD) represents the leading cause of visual impairment in the aging population. The goal of this study was to identify aberrantly-methylated, differentially-expressed genes (MDEGs) in AMD and explore the involved pathways via integrated bioinformatics analysis.Methods: Data from expression profile GSE29801 and methylation profile GSE102952 were obtained from the Gene Expression Omnibus database. We analyzed differentially-methylated genes and differentially-expressed genes using R software. Functional enrichment and protein–protein interaction (PPI) network analysis were performed using the R package and Search Tool for the Retrieval of Interacting Genes online database. Hub genes were identified using Cytoscape. Results: In total, 827 and 592 genes showed high and low expression, respectively, in GSE29801; 4117 hyper-methylated genes and 511 hypo-methylated genes were detected in GSE102952. Based on overlap, we categorized 153 genes as hyper-methylated, low-expression genes (Hyper-LGs) and 24 genes as hypo-methylated, high-expression genes (Hypo-HGs). Four Hyper-LGs (CKB, PPP3CA, TGFB2, SOCS2) overlapped with AMD risk genes in the Public Health Genomics and Precision Health Knowledge Base. KEGG pathway enrichment analysis indicated that Hypo-HGs were enriched in the calcium signaling pathway, whereas Hyper-LGs were enriched in sphingolipid metabolism. In GO analysis, Hypo-HGs were enriched in fibroblast migration, membrane raft, and coenzyme binding, among others. Hyper-LGs were enriched in mRNA transport, nuclear speck, and DNA binding, among others. In PPI network analysis, 23 nodes and two edges were established from Hypo-HGs, and 151 nodes and 73 edges were established from Hyper-LGs. Hub genes (DHX9, MAPT, PAX6) showed the greatest overlap. Conclusion: This study revealed potentially aberrantly MDEGs and pathways in AMD, which might improve the understanding of this disease.

scholarly journals The ocular tissue-specificity of differentially expressed age-related macular degeneration associated genes

2021 ◽  
Author(s):  
Dylan Duchen ◽  
Terri Beaty
Keyword(s):  
Macular Degeneration ◽  
Mrna Expression ◽  
Tissue Specificity ◽  
Retinal Pigment ◽  
Age Related Macular Degeneration ◽  
Differentially Expressed ◽  
Ocular Tissue ◽  
Genome Wide Association Studies ◽  
Ocular Tissues ◽  
Age Related

PurposeAge-related macular degeneration (AMD) is a leading cause of blindness in the developed world. One of the most genetically well-characterized degenerative diseases, genome-wide association studies (GWAS) have identified 52 independent common or rare AMD risk associated variants. While transcriptome-wide association analyses (TWAS) and expression quantitative trait loci (eQTL) efforts have characterized the effects of these AMD-associated genes on mRNA expression in retinal tissue, we aimed to characterize the AMD-associated transcriptional profiles of functionally distinct ocular tissues including the macular and extramacular regions of the retina and the retinal-pigment epithelium (RPE)/choroid.MethodsUsing publicly available microarray data (NCBI GEO accession: GSE29801) comprised of retinal and RPE/choroidal tissue samples from 142 AMD patients and 151 healthy individuals (118 retina and 175 RPE/Choroid samples), tissue-specific differential gene expression analyses were conducted. Transcriptome analyses were focused on 878 genes surrounding known AMD-associated loci.ResultsMany genes which contain clinically significant or causal variants identified via GWAS or TWAS/eQTL studies were significantly differentially expressed and display transcriptional heterogeneity across different subtypes of ocular tissue and retinal geography in AMD-associated tissues.ConclusionThese findings demonstrate the importance of spatial heterogeneity and tissue specificity in the mRNA expression of known AMD-associated genes. Genes known to harbor rare or causal AMD- associated variants are differentially expressed in functionally distinct ocular tissues of AMD patients, suggesting they might contribute to disease regardless of mutation status.

scholarly journals Down‐regulated tear film proteins in neovascular age‐related macular degeneration are involved in the regulation of protein clearance, inflammation, neovascularization and apoptosis

2021 ◽  
Vol 99 (S265) ◽  
Author(s):  
Mateusz Winiarczyk ◽  
Dagmara Winiarczyk ◽  
Katarzyna Michalak ◽  
Kai Kaarniranta ◽  
Jerzy Mackiewicz ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Tear Film ◽  
Age Related Macular Degeneration ◽  
Protein Clearance ◽  
Age Related
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