scholarly journals Multilocus sequence analysis of ‘Candidatus Phytoplasma asteris’ strain and the genome analysis of Turnip mosaic virus co-infecting oilseed rape

2014 ◽  
Vol 117 (3) ◽  
pp. 774-785 ◽  
Author(s):  
M. Šeruga Musić ◽  
H. Duc Nguyen ◽  
S. Černi ◽  
Đ. Mamula ◽  
K. Ohshima ◽  
...  

1999 ◽  
Vol 99 (7-8) ◽  
pp. 1149-1154 ◽  
Author(s):  
J. A. Walsh ◽  
A. G. Sharpe ◽  
C. E. Jenner ◽  
D. J. Lydiate




1996 ◽  
Vol 62 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Piyasak CHAUMPLUK ◽  
Yukiko SASAKI ◽  
Naoko NAKAJIMA ◽  
Hideaki NAGANO ◽  
Ikuo NAKAMURA ◽  
...  


2021 ◽  
Author(s):  
Mingzhen Zhang ◽  
Pan Gong ◽  
Linhao Ge ◽  
Zhaoyang Chang ◽  
Xiaofei Cheng ◽  
...  


Virus Genes ◽  
2021 ◽  
Vol 57 (2) ◽  
pp. 233-237
Author(s):  
Hendrik Reuper ◽  
Björn Krenz

AbstractTurnip mosaic virus (TuMV), belonging to the genus Potyvirus (family Potyviridae), has a large host range and consists of a single-stranded positive sense RNA genome encoding 12 proteins, including the P1 protease. This protein which is separated from the polyprotein by cis cleavage at its respective C-terminus, has been attributed with different functions during potyviral infection of plants. P1 of Turnip mosaic virus (P1-TuMV) harbors an FGSF-motif and FGSL-motif at its N-terminus. This motif is predicted to be a binding site for the host Ras GTPase-activating protein-binding protein (G3BP), which is a key factor for stress granule (SG) formation in mammalian systems and often targeted by viruses to inhibit SG formation. We therefore hypothesized that P1-TuMV might interact with G3BP to control and regulate plant SGs to optimize cellular conditions for the production of viral proteins. Here, we analyzed the co-localization of the Arabidopsis thaliana G3BP-2 with the P1 of two TuMV isolates, namely UK 1 and DEU 2. Surprisingly, P1-TuMV-DEU 2 co-localized with AtG3BP-2 under abiotic stress conditions, whereas P1-TuMV-UK 1 did not. AtG3BP-2::RFP showed strong SGs formation after stress, while P1-UK 1::eGFP maintained a chloroplastic signal under stress conditions, the signal of P1-DEU 2::eGFP co-localized with that of AtG3BP-2::RFP. This indicates a specific interaction between P1-DEU 2 and the AtG3BP family which is not solely based on the canonical interaction motifs.



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