Comparative analyses withinGyrodactylus(Platyhelminthes: Monogenea) mitochondrial genomes and conserved polymerase chain reaction primers for gyrodactylid mitochondrial DNA

2016 ◽  
Vol 40 (4) ◽  
pp. 541-555 ◽  
Author(s):  
F Ye ◽  
R H Easy ◽  
S D King ◽  
D K Cone ◽  
P You
Author(s):  
Danny A. Humphreys-Pereira ◽  
Taeho Kim ◽  
Joong-Ki Park

Abstract This chapter presents procedures on polymerase chain reaction (PCR) amplification, protocols for PCR, cloning and sequencing, and mitochondrial genome annotation and gene identification for the characterization of nematodes.


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