Authentication of Canned Tuna and Bonito by Sequence and Restriction Site Analysis of Polymerase Chain Reaction Products of Mitochondrial DNA

1996 ◽  
Vol 44 (8) ◽  
pp. 2460-2467 ◽  
Author(s):  
Jeffrey L. Ram ◽  
Michal L. Ram ◽  
Fadi F. Baidoun
Keyword(s):  
Polymerase Chain Reaction ◽  
Mitochondrial Dna ◽  
Restriction Site ◽  
Reaction Products ◽  
Chain Reaction ◽  
Site Analysis ◽  
Restriction Site Analysis ◽  
Canned Tuna ◽  
Polymerase Chain

Audiologic Features of Hearing Loss Due to the 1,555 Mutation of Mitochondrial DNA

1997 ◽  
Vol 106 (8) ◽  
pp. 643-648 ◽  
Author(s):  
Takashi Tsuiki ◽  
Kazuo Murai ◽  
Ken Kitamura ◽  
Seiko Murai ◽  
Yuya Tamagawa
Keyword(s):  
Polymerase Chain Reaction ◽  
Hearing Loss ◽  
Mitochondrial Dna ◽  
Direct Sequencing ◽  
High Tone ◽  
Reaction Products ◽  
Chain Reaction ◽  
Cycle Sequencing ◽  
Sequencing Method ◽  
Polymerase Chain

We proved a 1,555 mutation of mitochondrial DNA in one member of each of three families with familial streptomycin hearing loss, and report the pedigrees and audiologic features. DNA was extracted by the standard method. The 1,555 A to G mutation was identified in all three patients and confirmed by direct sequencing of the polymerase chain reaction products by a cycle sequencing method. On audiograms, the hearing loss was sensorineural, bilateral, and symmetric, showing a high-tone loss or a profound loss particularly in the high-tone range, and the “symmetry law” of Langenbeck was applicable. The superimposed audiograms of members of one family did not cross themselves, proving the applicability of the “never-cross principle of audiograms.”

One-Step Directional Cloning of Blunt-Ended Polymerase Chain Reaction Products

2000 ◽  
Vol 287 (2) ◽  
pp. 349-352 ◽  
Author(s):  
Vincent W.S. Liu ◽  
Phillip Nagley ◽  
Hextan Y.S. Ngan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Products ◽  
Chain Reaction ◽  
Directional Cloning ◽  
One Step ◽  
Polymerase Chain
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