Mucus piRNAs profiles of Vibrio harveyi ‐infected Cynoglossus semilaevis : A hint for fish disease monitoring

2021 ◽  
Author(s):  
Na Zhao ◽  
Qiuxia Deng ◽  
Chunhua Zhu ◽  
Bo Zhang
Keyword(s):  
Vibrio Harveyi ◽  
Cynoglossus Semilaevis ◽  
Disease Monitoring ◽  
Fish Disease

Molecular cloning and immune characterization of CIITA in artificially challenged Chinese tongue sole (Cynoglossus semilaevis) with Vibrio harveyi

2021 ◽  
pp. 104091
Author(s):  
Zhangfan Chen ◽  
Lei Wang ◽  
Xiwen Xu ◽  
Qian Zhou ◽  
Jie Wang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Vibrio Harveyi ◽  
Cynoglossus Semilaevis ◽  
Tongue Sole

scholarly journals Fish disease monitoring along a pollution transect: a case study using dab Limanda limanda in the German Bight

1992 ◽  
Vol 91 ◽  
pp. 173-192 ◽  
Author(s):  
AD Vethaak ◽  
D Bucke ◽  
T Lang ◽  
PW Wester ◽  
J Jol ◽  
...  
Keyword(s):  
German Bight ◽  
Disease Monitoring ◽  
Fish Disease

Genetic analysis of disease resistance to Vibrio harveyi by challenge test in Chinese tongue sole (Cynoglossus semilaevis)

Aquaculture ◽  
2019 ◽  
Vol 503 ◽  
pp. 430-435 ◽  
Author(s):  
Yangzhen Li ◽  
Lei Wang ◽  
Yingming Yang ◽  
Xue Li ◽  
Huan Dai ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Genetic Analysis ◽  
Vibrio Harveyi ◽  
Challenge Test ◽  
Cynoglossus Semilaevis ◽  
Tongue Sole

scholarly journals Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Hao Xu ◽  
Xiwen Xu ◽  
Xihong Li ◽  
Lei Wang ◽  
Jiayu Cheng ◽  
...  
Keyword(s):  
Immune Response ◽  
Molecular Mechanisms ◽  
Vibrio Harveyi ◽  
Bacterial Pathogen ◽  
Transcriptome Profiling ◽  
Transcript Level ◽  
Cynoglossus Semilaevis ◽  
Illumina Hiseq ◽  
Tongue Sole ◽  
And Control

Abstract Vibrio harveyi is a major bacterial pathogen that causes fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis), resulting in massive mortality in the farming industry. However, the molecular mechanisms of C. semilaevis response to V. harveyi infection are poorly understood. Here, we performed transcriptomic analysis of C. semilaevis, comparing resistant and susceptible families in response to V. harveyi challenge (CsRC and CsSC) and control conditions (CsRU and CsSU). RNA libraries were constructed using 12 RNA samples isolated from three biological replicates of the four groups. We performed transcriptome sequencing on an Illumina HiSeq platform, and generating a total of 1,095 million paired-end reads, with the number of clean reads per library ranging from 75.27 M to 99.97 M. Through pairwise comparisons among the four groups, we identified 713 genes exhibiting significant differences at the transcript level. Furthermore, the expression levels were validated by real-time qPCR. Our results provide a valuable resource and new insights into the immune response to V. harveyi infection.

Proteomics of mucosal exosomes of Cynoglossus semilaevis altered when infected by Vibrio harveyi

2021 ◽  
pp. 104045
Author(s):  
Na Zhao ◽  
Lei Jia ◽  
Xiaoxu He ◽  
Bo Zhang
Keyword(s):  
Vibrio Harveyi ◽  
Cynoglossus Semilaevis

scholarly journals Development of a 38K Single Nucleotide Polymorphism Array and Application in Henomic Selection for Resistance Against Vibrio harveyi in Chinese Tongue sole, Cynoglossus Semilaevis

2020 ◽  
Author(s):  
Sheng Lu ◽  
Qian Zhou ◽  
Yadong Chen ◽  
Yang Liu ◽  
Yangzhen Li ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Vibrio Harveyi ◽  
Reference Group ◽  
Single Nucleotide Polymorphism Array ◽  
Snp Array ◽  
Cynoglossus Semilaevis ◽  
Breeding Value ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Tongue Sole

Abstract Background: In recent years, the disease outbreak caused by Vibrio harveyi upset the booming development of the Chinese tongue sole (Cynoglossus semilaevis) farming industry. Genomic selection (GS) is a powerful method to improve the traits of interest, which has been proved in livestock and some fishes. Besides, the single nucleotide polymorphism (SNP) array is an efficient genotyping platform that can be used for genetic studies. To improve V. harveyi resistance in C. semilaevis, we firstly constructed a reference group of 1,572 individuals and investigated accuracies of four genomic methods (genomic best linear unbiased prediction (GBLUP), weighted GBLUP, BayesB, and BayesC) at predicting the genomic estimated breeding value (GEBV) using five-fold cross-validation and SNPs varying from 0.5 k to 500 k. Then, an SNP array was developed using the Affymetrix Axiom technology, and its accuracy in genotyping was evaluated by comparing SNPs generated by the array and by the re-sequencing technology. Finally, we selected 44 candidates as the parents of 23 families of C. semilaevis to evaluate the feasibility of the SNP array for GS.Results: all genomic methods outperformed the pedigree-based BLUP (ABLUP) when at least 50 k SNPs used for prediction, of which GBLUP resulted in better estimation than ABLUP when more than 1 k SNPs used. A 38 k SNP array, “Solechip No.1”, was developed with an average of 10.5 kb inter-spacing between two adjacent SNPs. The SNPs generated by the array and by the re-sequencing reached an average consistency of 94.8 %, of which 79.3 % of loci had a more than 90 % of the consistency. The survival rates of these 23 offspring families had a correlation of 0.706 with the family GEBVs (mid-parental GEBVs), and the average survival rate of the top five families in GEBVs (79.1 %) is higher than the bottom five families (58.1 %).Conclusion: GS is an efficient method to improve the V. harveyi resistance in C. semilaevis, and the SNP array “Solechip No.1” is a convenient and reliable tool for the Chinses tongue sole selective breeding practice.

Detection of SSR and a QTL analysis of Vibrio harveyi resistance in Cynoglossus semilaevis

2017 ◽  
Vol 24 (1) ◽  
pp. 22 ◽  
Author(s):  
Huan DAI ◽  
Yang LIU ◽  
Wenwen WANG ◽  
Zhanfei WEI ◽  
Jin GAO ◽  
...  
Keyword(s):  
Qtl Analysis ◽  
Vibrio Harveyi ◽  
Cynoglossus Semilaevis

Detecting Cynoglossus semilaevis infected with Vibrio harveyi using micro RNAs from mucous exosomes

2020 ◽  
Vol 128 ◽  
pp. 268-276
Author(s):  
Na Zhao ◽  
Bo Zhang ◽  
Zihui Xu ◽  
Lei Jia ◽  
Ming Li ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Cynoglossus Semilaevis ◽  
Micro Rnas

Fish Disease Monitoring in the Dutch Part of the North Sea in Relation to the Dumping of Waste From Titanium Dioxide Production

1991 ◽  
Vol 5 (3) ◽  
pp. 149-170 ◽  
Author(s):  
Dick Vethaak ◽  
Jaap Van Der Meer
Keyword(s):  
Titanium Dioxide ◽  
North Sea ◽  
Disease Monitoring ◽  
Fish Disease ◽  
The North ◽  
The North Sea
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