Isolation and Characterization of naringinase enzyme and its application in debittering of Pomelo juice ( Citrus grandis ): A comparative study with macroporous resin

2021 ◽  
Author(s):  
Arun Kumar Gupta ◽  
Monica Yumnam ◽  
Manisha Medhi ◽  
Parismita Koch ◽  
Sourav Chakraborty ◽  
...  
Keyword(s):  
Comparative Study ◽  
Macroporous Resin ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of ardicrenin from Ardisia crenata Sims

2009 ◽  
Vol 55 (No. 7) ◽  
pp. 305-310 ◽  
Author(s):  
Ma Yuan ◽  
Pu Shangrao ◽  
Cheng Qingsu ◽  
Ma Mingdong
Keyword(s):  
High Performance ◽  
Amorphous Powder ◽  
Gel Chromatography ◽  
Macroporous Resin ◽  
Resin Column ◽  
Isolation And Characterization ◽  
Natural Reserve ◽  
Silica Gel Chromatography ◽  
Counter Current

A new, effective and economical method to extract ardicrenin from <I>Ardisia crenata</I> Sims collected in the Wolong natural reserve, Sichuan, China, is established. <I>Ardisia crenata</I> Sims powder is counter-current extracted with 80% methanol reflux, decompressively enriched and centrifuged to defat. Supernatant is applied to macroporous resin column (AB-8) with 80% methanol, ardicrenin is isolated by silica gel chromatography with dichlormethane-acetoacetate-methanol (4:1.5:1) washing, and recrystallized in methanol. The final product which proved to be ardicrenin by analytic procedure including Furier transform infrared (FTIR) and ultraviolet spectrum (UV), mass spectroscopy (MS), nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC) is white amorphous powder with yield of 1.59 ± 0.02%.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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