scholarly journals Stereological analysis of individual lung lobes during normal and aberrant mouse lung alveolarisation

2018 ◽  
Vol 232 (3) ◽  
pp. 472-484 ◽  
Author(s):  
Tuong-Van Hoang ◽  
Claudio Nardiello ◽  
David E. Surate Solaligue ◽  
José Alberto Rodríguez-Castillo ◽  
Philipp Rath ◽  
...  
Keyword(s):  
Mouse Lung ◽  
Stereological Analysis ◽  
Lung Lobes

Stereological analysis of single lung lobes in newborn mice exposed to hyperoxia

2017 ◽  
Author(s):  
Tuong-Van Hoang ◽  
Claudio Nardiello ◽  
David E. Surate Solaligue ◽  
Alberto Rodríguez-Castillo ◽  
Werner Seeger ◽  
...  
Keyword(s):  
Newborn Mice ◽  
Stereological Analysis ◽  
Lung Lobes

Estimation of 100 nm particle distribution kinetics in mouse lung using confocal laser scanning microscopy

2021 ◽  
Vol 21 (3) ◽  
pp. 103-108
Author(s):  
Julia D. Vavilova ◽  
Elena L. Bolkhovitina ◽  
Andrey O. Bogorodskiy ◽  
Ivan S. Okhrimenko ◽  
Valentin I. Borshchevskiy ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Laser Scanning ◽  
Particle Distribution ◽  
Laser Scanning Microscopy ◽  
Mouse Lung ◽  
Confocal Laser ◽  
Phagocytic Cells ◽  
Viral Particles ◽  
Mouse Lungs ◽  
Lung Lobes

BACKGROUND: Daily, people inhale airborne viral particles, some of which have a size of about 100 nm, such as particles of SARS-CoV-2. Kinetics of such 100 nm particle distribution in the respiratory tract is important, however, not a properly investigated question. AIM: To estimate the dissemination of inert viral particles based on the analysis of the spatial distribution of fluorescent 100 nm particles in the mouse lungs at different time points after the application. MATHERIALS AND METHODS: Fluorescent particles of 100 nm size were applied to C57BL/6 mice. 6, 24, 48 and 72 hours after, lungs were excised and fixed. Lung lobes were stained with immunohistochemistry as whole-mounts and then underwent optical clearance. Three-dimensional images of whole-mount mouse lung lobes were acquired using confocal laser scanning microscopy. RESULTS: 6 hours after the particle application particles were detected in lungs both as single particles and as particle agglomerates. Particles were both free and internalized by phagocytic cells. 24 hours after the application particles were detected both in bronchial lumen and in the alveolar space. Particles were detected in the mouse lungs up to 72 hours after the application. CONCLUSIONS: Reaching the respiratory tract of mammalian, inert particles which size equal to SARS-CoV-2 particle size distribute both in bronchi and in alveoli and undergoes internalization of phagocytic cells.

Protocol for CUBIC Clearing and Whole Mount Imaging of Mouse Lung Lobes v1

2021 ◽  
Author(s):  
Justinn Barr ◽  
Jamie Verheyden ◽  
Xin Sun
Keyword(s):  
Laboratory Animal ◽  
Computational Analysis ◽  
American Association ◽  
Mouse Lung ◽  
Whole Mount ◽  
Mouse Lung Tissue ◽  
Laboratory Animal Facility ◽  
The University ◽  
Animal Care ◽  
Lung Lobes

This protocol is for Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis (CUBIC) of mouse lung tissue for whole lobe imaging using Zeiss Lightsheet Imaging. All experimental procedures were performed in the American Association for Accreditation of Laboratory Animal Care (AAALAC)-certified laboratory animal facility at the University of California San Diego, following protocols approved by the institutional animal care and use committee (IACUC). The procedures should incorporate all local requirements for standards of animal experimentation.

Imaging of the mouse lung with scanning laser optical tomography (SLOT)

2012 ◽  
Vol 113 (6) ◽  
pp. 975-983 ◽  
Author(s):  
Manuela Kellner ◽  
Marko Heidrich ◽  
Rebecca Beigel ◽  
Raoul-Amadeus Lorbeer ◽  
Lars Knudsen ◽  
...  
Keyword(s):  
Internal Structure ◽  
Ex Vivo ◽  
Optical Tomography ◽  
Three Dimensional ◽  
Scanning Laser ◽  
Mouse Lung ◽  
Microscopy Technique ◽  
Planar Orientation ◽  
Scan Modes ◽  
Lung Lobes

The current study focuses on the use of scanning laser optical tomography (SLOT) in imaging of the mouse lung ex vivo. SLOT is a highly efficient fluorescence microscopy technique allowing rapid scanning of samples of a size of several millimeters, thus enabling volumetric visualization by using intrinsic contrast mechanisms of previously fixed lung lobes. Here, we demonstrate the imaging of airways, blood vessels, and parenchyma from whole, optically cleared mouse lung lobes with a resolution down to the level of single alveoli using absorption and autofluorescence scan modes. The internal structure of the lung can then be analyzed nondestructively and quantitatively in three-dimensional datasets in any preferred planar orientation. Moreover, the procedure preserves the microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, the current study has shown that SLOT is a valuable technique to study the internal structure of the mouse lung.

Stereological analysis of the flag leaf of someTriticumL. species

2006 ◽  
Vol 34 (2-3) ◽  
pp. 1005-1012 ◽  
Author(s):  
Jadranka Luković
Keyword(s):  
Flag Leaf ◽  
Stereological Analysis
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