scholarly journals The apparent non-host resistance of Ethiopian mustard to a radish-infecting strain of Turnip mosaic virus is largely determined by the C-terminal region of the P3 viral protein

2018 ◽  
Vol 19 (8) ◽  
pp. 1984-1994 ◽  
Author(s):  
Papaiah Sardaru ◽  
Laura Sinausía ◽  
Silvia López-González ◽  
Jelena Zindovic ◽  
Flora Sánchez ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Host Resistance ◽  
Viral Protein ◽  
Turnip Mosaic Virus ◽  
Terminal Region ◽  
Ethiopian Mustard

Molecular Characterization of the 3'-Terminal Region of Turnip mosaic virus Isolates from Eastern China

2007 ◽  
Vol 155 (6) ◽  
pp. 333-341 ◽  
Author(s):  
Y.-P. Tian ◽  
X.-P. Zhu ◽  
J.-L. Liu ◽  
X.-Q. Yu ◽  
J. Du ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Eastern China ◽  
Turnip Mosaic Virus ◽  
Terminal Region ◽  
Virus Isolates

scholarly journals First Report of Turnip mosaic virus Infecting Brassica carinata (Ethiopian Mustard) in the United States

Plant Disease ◽  
2013 ◽  
Vol 97 (12) ◽  
pp. 1664-1664 ◽  
Author(s):  
B. Babu ◽  
H. Dankers ◽  
S. George ◽  
D. Wright ◽  
J. Marois ◽  
...  
Keyword(s):  
United States ◽  
Mosaic Virus ◽  
The United States ◽  
Turnip Mosaic Virus ◽  
Brassica Carinata ◽  
Rt Pcr ◽  
First Report ◽  
Ethiopian Mustard ◽  
Plant Pathol ◽  
Pcr Assays

Brassica carinata L. Braun (Ethiopian mustard) is an annual oil seed crop currently being evaluated for its potential use as a source of biofuel. Due to its high content of erucic acid, it provides a biodegradable non-fossil fuel feedstock that has many applications ranging from biofuels to other industrial uses such as polymers, waxes, and surfactants. Moreover, high glucosinolate content adds the scope of B. carinata being used as a bio-fumigant. B. carinata is amenable to low input agriculture and has great economic potential to be used as a winter crop, especially in the southeastern United States. Virus-like leaf symptoms including mosaic, ringspot, mottling, and puckering were observed on B. carinata (cvs. 080814 EM and 080880 EM) in field trials at Quincy, FL, during spring 2013, with disease incidence of >80%. A more extensive survey of the same field location indicated that mosaic symptoms were the most common. Viral inclusion assays (1) of leaves with a range of symptoms indicated the presence of potyvirus-like inclusion bodies. Total RNA extracts (RNeasy Plant Mini Kit, Qiagen Inc., Valencia, CA) from six symptomatic samples and one non-symptomatic B. carinata sample were subjected to reverse transcription (RT)-PCR assays using SuperScript III One-Step RT-PCR System (Invitrogen, Life Technologies, NY), and two sets of potyvirus-specific degenerate primers MJ1-F and MJ2-R (2) and NIb2F and NIb3R (3), targeting the core region of the CP and NIb, respectively. The RT-PCR assays using the CP and NIb specific primers produced amplicons of 327 bp and 350 bp, respectively, only in the symptomatic leaf samples. The obtained amplicons were gel-eluted and sequenced directly (GenBank Accession Nos. KC899803 to KC899808 for CP and KC899809 to KC899813 for NIb). BLAST analysis of these sequences revealed that they came from Turnip mosaic virus (TuMV). Pairwise comparisons of the CP (327 bp) and NIb (350 bp) segments revealed 98 to 99% and 96 to 98% nucleotide identities, respectively, with corresponding sequences of TuMV isolates. These results revealed the association of TuMV with symptomatic B. carinata leaf samples. Although TuMV has been reported from B. carinata in Zambia (4), this is the first report of its occurrence on B. carinata in the United States. Considering the importance of B. carinata as a biofuel source, this report underscores the need for developing effective virus management strategies for the crop. References: (1) R. G. Christie and J. R. Edwardson. Plant Dis. 70:273, 1986. (2) M. Grisoni et al. Plant Pathol. 55:523, 2006. (3) L. Zheng et al. Plant Pathol. 59:211, 2009. (4) D. S. Mingochi and A. Jensen. Acta Hortic. 218:289, 1988.

scholarly journals Visualization of the Interaction between the Precursors of VPg, the Viral Protein Linked to the Genome of Turnip Mosaic Virus, and the Translation Eukaryotic Initiation Factor iso 4E In Planta

2006 ◽  
Vol 81 (2) ◽  
pp. 775-782 ◽  
Author(s):  
Chantal Beauchemin ◽  
Nathalie Boutet ◽  
Jean-François Laliberté
Keyword(s):  
Mosaic Virus ◽  
Viral Protein ◽  
Fluorescent Protein ◽  
Turnip Mosaic Virus ◽  
Bimolecular Fluorescence Complementation ◽  
Initiation Factor ◽  
Eukaryotic Initiation Factor ◽  
In Planta ◽  
Translation Factor ◽  
Cytoplasmic Vesicles

ABSTRACT The RNA genome of Turnip mosaic virus is covalently linked at its 5′ end to a viral protein known as VPg. This protein binds to the translation eukaryotic initiation factor iso 4E [eIF(iso)4E]. This interaction has been shown to be important for virus infection, although its exact biological function(s) has not been elucidated. In this study, we investigated the subcellular site of the VPg-eIF(iso)4E interaction using bimolecular fluorescence complementation (BiFC). As a first step, eIF(iso)4E, 6K-VPg-Pro, and VPg-Pro were expressed as full-length green fluorescent protein (GFP) fusions in Nicotiana benthamiana, and their subcellular localizations were visualized by confocal microscopy. eIF(iso)4E was predominantly associated with the endoplasmic reticulum (ER), and VPg-Pro was observed in the nucleus and possibly the nucleolus, while 6K-VPg-Pro-GFP induced the formation of cytoplasmic vesicles budding from the ER. In BiFC experiments, reconstituted green fluorescence was observed throughout the nucleus, with a preferential accumulation in subnuclear structures when the GFP split fragments were fused to VPg-Pro and eIF(iso)4E. On the other hand, the interaction of 6K-VPg-Pro with eIF(iso)4E was observed in cytoplasmic vesicles embedded in the ER. These data suggest that the association of VPg with the translation factor might be needed for two different functions, depending of the VPg precursor involved in the interaction. VPg-Pro interaction with eIF(iso)4E may be involved in perturbing normal cellular functions, while 6K-VPg-Pro interaction with the translation factor may be needed for viral RNA translation and/or replication.

scholarly journals The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex

Virology ◽  
2017 ◽  
Vol 510 ◽  
pp. 147-155 ◽  
Author(s):  
Xiaoyan Cui ◽  
Hoda Yaghmaiean ◽  
Guanwei Wu ◽  
Xiaoyun Wu ◽  
Xin Chen ◽  
...  
Keyword(s):  
Viral Replication ◽  
Viral Infection ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Terminal Region ◽  
Replication Complex ◽  
Viral Replication Complex

A fitness cost for Turnip mosaic virus to overcome host resistance

2002 ◽  
Vol 86 (1-2) ◽  
pp. 1-6 ◽  
Author(s):  
Carol E. Jenner ◽  
Xiaowu Wang ◽  
Fernando Ponz ◽  
John A. Walsh
Keyword(s):  
Mosaic Virus ◽  
Host Resistance ◽  
Turnip Mosaic Virus ◽  
Fitness Cost
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