ArabidopsisHSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses

2014 ◽  
Vol 202 (4) ◽  
pp. 1320-1334 ◽  
Author(s):  
Fei Bao ◽  
Xiaozhen Huang ◽  
Chipan Zhu ◽  
Xiaoyan Zhang ◽  
Xin Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Defense Responses ◽  
Temperature Dependent ◽  
Dependent Cell

scholarly journals The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death

2010 ◽  
Vol 187 (2) ◽  
pp. 301-312 ◽  
Author(s):  
Xiaozhen Huang ◽  
Yansha Li ◽  
Xiaoyan Zhang ◽  
Jianru Zuo ◽  
Shuhua Yang
Keyword(s):  
Cell Death ◽  
Low Temperature ◽  
Temperature Dependent ◽  
Dependent Cell

scholarly journals Optimizing the PBS1 Decoy System to Confer Resistance to Potyvirus Infection in Arabidopsis and Soybean

2020 ◽  
Vol 33 (7) ◽  
pp. 932-944 ◽  
Author(s):  
Sarah E. Pottinger ◽  
Aurelie Bak ◽  
Alexandra Margets ◽  
Matthew Helm ◽  
Lucas Tang ◽  
...  
Keyword(s):  
Cell Death ◽  
Plasma Membrane ◽  
Mosaic Virus ◽  
Pseudomonas Syringae ◽  
Soybean Mosaic Virus ◽  
Aphid Transmission ◽  
Defense Responses ◽  
Cell Death Response ◽  
Dependent Cell ◽  
Nia Protease

The Arabidopsis resistance protein RPS5 is activated by proteolytic cleavage of the protein kinase PBS1 by the Pseudomonas syringae effector protease AvrPphB. We have previously shown that replacing seven amino acids at the cleavage site of PBS1 with a motif cleaved by the NIa protease of turnip mosaic virus (TuMV) enables RPS5 activation upon TuMV infection. However, this engineered resistance conferred a trailing necrosis phenotype indicative of a cell-death response too slow to contain the virus. We theorized this could result from a positional mismatch within the cell between PBS1TuMV, RPS5, and the NIa protease. To test this, we relocalized PBS1TuMV and RPS5 to cellular sites of NIa accumulation. These experiments revealed that relocation of RPS5 away from the plasma membrane compromised RPS5-dependent cell death in Nicotiana benthamiana, even though PBS1 was efficiently cleaved. As an alternative approach, we tested whether overexpression of plasma membrane–localized PBS1TuMV could enhance RPS5 activation by TuMV. Significantly, overexpressing the PBS1TuMV decoy protein conferred complete resistance to TuMV when delivered by either agrobacterium or by aphid transmission, showing that RPS5-mediated defense responses are effective against bacterial and viral pathogens. Lastly, we have now extended this PBS1 decoy approach to soybean by modifying a soybean PBS1 ortholog to be cleaved by the NIa protease of soybean mosaic virus (SMV). Transgenic overexpression of this soybean PBS1 decoy conferred immunity to SMV, demonstrating that we can use endogenous PBS1 proteins in crop plants to engineer economically relevant disease resistant traits.

scholarly journals MEKK1 Is Required for MPK4 Activation and Regulates Tissue-specific and Temperature-dependent Cell Death inArabidopsis

2006 ◽  
Vol 281 (48) ◽  
pp. 36969-36976 ◽  
Author(s):  
Kazuya Ichimura ◽  
Catarina Casais ◽  
Scott C. Peck ◽  
Kazuo Shinozaki ◽  
Ken Shirasu
Keyword(s):  
Cell Death ◽  
Temperature Dependent ◽  
Tissue Specific ◽  
Dependent Cell

scholarly journals Optimizing the PBS1 Decoy System to Confer Resistance to Potyvirus Infection in Arabidopsis and Soybean

2020 ◽  
Author(s):  
Sarah E. Pottinger ◽  
Aurelie Bak ◽  
Alexandra Margets ◽  
Matthew Helm ◽  
Lucas Tang ◽  
...  
Keyword(s):  
Cell Death ◽  
Plasma Membrane ◽  
Mosaic Virus ◽  
Pseudomonas Syringae ◽  
Soybean Mosaic Virus ◽  
Aphid Transmission ◽  
Defense Responses ◽  
Cell Death Response ◽  
Dependent Cell ◽  
Nia Protease

ABSTRACTThe Arabidopsis resistance protein RPS5 is activated by proteolytic cleavage of the protein kinase PBS1 by the Pseudomonas syringae effector protease AvrPphB. We have previously shown that replacing seven amino acids at the cleavage site of PBS1 with a motif cleaved by the NIa protease of turnip mosaic virus (TuMV) enables RPS5 activation upon TuMV infection. However, this engineered resistance conferred a trailing necrosis phenotype indicative of a cell death response too slow to contain the virus. We theorized this could result from a positional mismatch within the cell between PBS1TuMV, RPS5 and the NIa protease. To test this, we re-localized PBS1TuMV and RPS5 to cellular sites of NIa accumulation. These experiments revealed that relocation of RPS5 away from the plasma membrane compromised RPS5-dependent cell death in N. benthamiana, even though PBS1 was efficiently cleaved. As an alternative approach, we tested whether overexpression of plasma membrane-localized PBS1TuMV would enhance RPS5 activation by TuMV. Significantly, over-expressing the PBS1TuMV decoy protein conferred complete resistance to TuMV when delivered by either Agrobacterium or by aphid transmission, showing that RPS5-mediated defense responses are effective against bacterial and viral pathogens. Lastly, we have now extended this PBS1 decoy approach to soybean by modifying a soybean PBS1 ortholog to be cleaved by the NIa protease of soybean mosaic virus (SMV). Transgenic overexpression of this soybean PBS1 decoy conferred immunity to SMV, demonstrating that we can use endogenous PBS1 proteins in crop plants to engineer economically relevant disease resistant traits.

scholarly journals Temperature-dependent cell death patterns induced by functionalized gold nanoparticle photothermal therapy in melanoma cells

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Yujuan Zhang ◽  
Xuelin Zhan ◽  
Juan Xiong ◽  
Shanshan Peng ◽  
Wei Huang ◽  
...  
Keyword(s):  
Cell Death ◽  
Gold Nanoparticle ◽  
Photothermal Therapy ◽  
Melanoma Cells ◽  
Temperature Dependent ◽  
Dependent Cell
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