scholarly journals Gene expression analysis of Cyanophora paradoxa reveals conserved abiotic stress responses between basal algae and flowering plants

2019 ◽  
Vol 225 (4) ◽  
pp. 1562-1577 ◽  
Author(s):  
Camilla Ferrari ◽  
Marek Mutwil
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Expression Analysis ◽  
Stress Responses ◽  
Gene Expression Analysis ◽  
Flowering Plants ◽  
Cyanophora Paradoxa

scholarly journals Global Gene-expression Analysis of the Response of Salmonella Enteritidis to Egg White Exposure Reveals Multiple Egg White-imposed Stress Responses

2017 ◽  
Vol 8 ◽  
Author(s):  
Florence Baron ◽  
Sylvie Bonnassie ◽  
Mariah Alabdeh ◽  
Marie-Françoise Cochet ◽  
Françoise Nau ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Stress Responses ◽  
Salmonella Enteritidis ◽  
Gene Expression Analysis ◽  
Global Gene Expression ◽  
Egg White ◽  
Global Gene Expression Analysis

scholarly journals Identification and selection of optimal reference genes for qPCR-based gene expression analysis in Fucus distichus under various abiotic stresses

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0233249
Author(s):  
Marina Linardić ◽  
Siobhan A. Braybrook
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Stress Responses ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Elongation Factor ◽  
Molecular Tools ◽  
Quantitative Gene Expression ◽  
Fucus Distichus ◽  
Elongation Factor 1

Quantitative gene expression analysis is an important tool in the scientist’s belt. The identification of evenly expressed reference genes is necessary for accurate quantitative gene expression analysis, whether by traditional RT-PCR (reverse-transcription polymerase chain reaction) or by qRT-PCR (quantitative real-time PCR; qPCR). In the Stramenopiles (the major line of eukaryotes that includes brown algae) there is a noted lack of known reference genes for such studies, largely due to the absence of available molecular tools. Here we present a set of nine reference genes (Elongation Factor 1 alpha (EF1A), Elongation Factor 2 alpha (EF2A), Elongation Factor 1 beta (EF1B), 14-3-3 Protein, Ubiquitin Conjugating Enzyme (UBCE2), Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH), Actin Related Protein Complex (ARP2/3), Ribosomal Protein (40s; S23), and Actin) for the brown alga Fucus distichus. These reference genes were tested on adult sporophytes across six abiotic stress conditions (desiccation, light and temperature modification, hormone addition, pollutant exposure, nutrient addition, and wounding). Suitability of these genes as reference genes was quantitatively evaluated across conditions using standard methods and the majority of the tested genes were evaluated favorably. However, we show that normalization genes should be chosen on a condition-by-condition basis. We provide a recommendation that at least two reference genes be used per experiment, a list of recommended pairs for the conditions tested here, and a procedure for identifying a suitable set for an experimenter’s unique design. With the recent expansion of interest in brown algal biology and accompanied molecular tools development, the variety of experimental conditions tested here makes this study a valuable resource for future work in basic biology and understanding stress responses in the brown algal lineage.

scholarly journals Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 222 ◽  
Author(s):  
Konstantinos Kagias ◽  
Agnieszka Podolska ◽  
Roger Pocock
Keyword(s):  
Gene Expression ◽  
Caenorhabditis Elegans ◽  
Expression Analysis ◽  
Stress Responses ◽  
Gene Expression Analysis ◽  
Quantitative Gene Expression ◽  
Reference Mirnas

scholarly journals Identification and selection of optimal reference genes for qPCR-based gene expression analysis in Fucus distichus under various abiotic stresses

2020 ◽  
Author(s):  
Marina Linardić ◽  
Siobhan A Braybrook
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Stress Responses ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Elongation Factor ◽  
Molecular Tools ◽  
Quantitative Gene Expression ◽  
Fucus Distichus ◽  
Elongation Factor 1

AbstractQuantitative gene expression analysis is an important tool in the scientist’s belt. The identification of evenly expressed reference genes is necessary for accurate quantitative gene expression analysis, whether by traditional RT-PCR (reverse-transcription polymerase chain reaction) or by qRT-PCR (quantitative real-time PCR; qPCR). In the Stramenopiles (the major line of eukaryotes that includes brown algae) there is a noted lack of known reference genes for such studies, largely due to the absence of available molecular tools. Here we present a set of nine reference genes (Elongation Factor 1 alpha (EF1A), Elongation Factor 2 alpha (EF2A), Elongation Factor 1 beta (EF1B), 14-3-3 Protein, Ubiquitin Conjugating Enzyme (UBCE2), Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH), Actin Related Protein Complex (ARP2/3), Ribosomal Protein (40s; S23), and Actin) for the brown alga Fucus distichus. These reference genes were tested on adult sporophytes across six abiotic stress conditions (desiccation, light and temperature modification, hormone addition, pollutant exposure, nutrient addition, and wounding). Suitability of these genes as reference genes was quantitatively evaluated across conditions using standard methods and the majority of the tested genes were evaluated favorably. However, we show that normalization genes should be chosen on a condition-by-condition basis. We provide a recommendation that at least two reference genes be used per experiment, a list of recommended pairs for the conditions tested here, and a procedure for identifying a suitable set for an experimenter’s unique design. With the recent expansion of interest in brown algal biology and accompanied molecular tools development, the variety of experimental conditions tested here makes this study a valuable resource for future work in basic biology and understanding stress responses in the brown algal lineage.

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