The PYR‐PP2C‐CKL2 module regulates ABA‐mediated actin reorganization during stomatal closure

2021 ◽  
Author(s):  
Yue Shi ◽  
Xiangning Liu ◽  
Shuangshuang Zhao ◽  
Yan Guo
Keyword(s):  
Stomatal Closure ◽  
Actin Reorganization

Nitric oxide, actin reorganization and vacuoles change are involved in PEG 6000-induced stomatal closure inVicia faba

2009 ◽  
Vol 136 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Ai-Xia Huang ◽  
Xiao-Ping She ◽  
Bin Cao ◽  
Bei Zhang ◽  
Juan Mu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stomatal Closure ◽  
Actin Reorganization ◽  
Peg 6000

scholarly journals Establishing the role of SINE proteins in regulating stomatal dynamics in Arabidopsis thaliana

2019 ◽  
Author(s):  
Alecia Biel ◽  
Morgan Moser ◽  
Iris Meier
Keyword(s):  
Nuclear Envelope ◽  
Stomatal Closure ◽  
Large Degree ◽  
Stress Hormone ◽  
Linc Complex ◽  
Biotic And Abiotic Stresses ◽  
Actin Reorganization ◽  
Stomatal Regulation ◽  
Opening And Closing

AbstractStomatal movement, which regulates gas exchange in plants, is controlled by a variety of environmental factors, including biotic and abiotic stresses. The stress hormone ABA initiates a signaling cascade, which leads to increased H2O2 and Ca2+ levels and F-actin reorganization, but the mechanism of, and connection between, these events is unclear. SINE1, an outer nuclear envelope component of a plant Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, associates with F-actin and is, along with its paralog SINE2, expressed in guard cells. Here, we have determined that Arabidopsis SINE1 and SINE2 play an important role in stomatal regulation. We show that SINE1 and SINE2 are required for stomatal opening and closing. Loss of SINE1 or SINE2 results in ABA hyposensitivity and impaired stomatal dynamics but does not affect stomatal closure induced by the bacterial elicitor flg22. The ABA-induced stomatal closure phenotype is, in part, attributed to impairments in Ca2+ and F-actin regulation. Together, the data suggest that SINE1 and SINE2 act downstream of ABA but upstream of Ca2+ and F-actin. While there is a large degree of functional overlap between the two proteins, there are also critical differences. Our study makes an unanticipated connection between stomatal regulation and a novel class of nuclear envelope proteins, and adds two new players to the increasingly complex system of guard cell regulation.

scholarly journals A NET4-RabG3 couple mediate the link between actin and the tonoplast and is essential for normal actin cytoskeletal remodelling in stomatal closure to flg22

2021 ◽  
Author(s):  
Timothy J HAWKINS ◽  
Michaela Kopischke ◽  
David Mentlak ◽  
Patrick Duckney ◽  
Johan Kroon ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Guard Cell ◽  
Actin Filaments ◽  
Plant Immunity ◽  
Stomatal Closure ◽  
Actin Network ◽  
Actin Reorganization ◽  
Downstream Effectors ◽  
Membrane Bound

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. They tether the cell's plasma membrane (PM) to the actin network. Moreover, in a similar manner, they are also involved in the tethering of membrane bound organelles to the actin cytoskeleton; the endoplasmic reticulum (ER) and the ER to the PM. This raises the question as to whether NET proteins are involved in actin cytoskeletal remodelling. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal closure in plant immunity. NET4 proteins interact with F-actin and with members of the Rab7 GTPase RABG3 family through two distinct domains, allowing for simultaneous localization to actin filaments and the tonoplast. NET4 proteins interact with GTP-bound, active RABG3 members, suggesting their function as downstream effectors. We also show that RABG3b is critical for stomatal closure induced by microbial patterns. Taken together, we conclude that the actin cytoskeletal remodelling during stomatal closure depends on a molecular link between actin filaments and the tonoplast, which is mediated by the NET4-RABG3b interaction. We propose that stomatal closure to microbial patterns involves the coordinated action of immune signalling events and proper actin cytoskeletal remodelling.

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