Differences in RUNX 2 and P2 RX 7 gene expression between mono‐ and coculture of human periodontal ligament cells and human osteoblasts under compressive force application

2019 ◽  
Vol 22 (3) ◽  
pp. 168-176 ◽  
Author(s):  
Jianwei Shi ◽  
Matthias Folwaczny ◽  
Andrea Wichelhaus ◽  
Uwe Baumert
Keyword(s):  
Gene Expression ◽  
Periodontal Ligament ◽  
Compressive Force ◽  
Periodontal Ligament Cells ◽  
Human Osteoblasts ◽  
Force Application ◽  
Human Periodontal Ligament Cells

scholarly journals Force-induced decline of FOXM1 in human periodontal ligament cells contributes to osteoclast differentiation

2019 ◽  
Vol 89 (5) ◽  
pp. 804-811 ◽  
Author(s):  
Qian Li ◽  
Jianyun Zhang ◽  
Dawei Liu ◽  
Yunan Liu ◽  
Yanheng Zhou
Keyword(s):  
Transcription Factors ◽  
Periodontal Ligament ◽  
Osteoclast Differentiation ◽  
Specific Inhibitor ◽  
Compressive Force ◽  
Nuclear Factor Κb ◽  
Periodontal Ligament Cells ◽  
Tartrate Resistant Acid Phosphatase ◽  
Proliferation Capacity ◽  
Human Periodontal Ligament Cells

ABSTRACT Objectives: To investigate whether Forkhead family transcription factors are responsive to mechanical force and the resulting influence on the osteoclast differentiation mediated by human periodontal ligament cells (PDLCs). Materials and Methods: A high-throughput RNA sequencing assay was performed in compressive force–stimulated and control human PDLCs. Alteration of FOXM1, a member of the Forkhead family transcription factors, was further confirmed by Western blotting and quantitative reverse-transcription polymerase chain reaction. Expression of FOXM1 was inhibited by either small interfering RNA (siRNA) transfection or addition of its specific inhibitor Siomycin A. Then, cells were exposed to compressive force and co-cultured with the murine macrophage cell line Raw264.7, followed by tartrate-resistant acid phosphatase staining assay. Expression changes of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegetin (OPG) caused by FOXM1 suppression were measured. Alkaline phosphatase (ALP) staining, ALP activity assay, and crystal violet staining assay were performed after FOXM1 inhibition. Results: FOXM1 transcription decreased after mechanical stimulation in PDLCs. Inhibition of FOXM1 promoted force-induced osteoclast differentiation of RAW264.7 and upregulated the RANKL/OPG ratio in PDLCs. Interference of FOXM1 led to promoted osteogenic differentiation but decreased proliferation of PDLCs. Conclusions: FOXM1 is a novel mechano-responsive gene in human PDLCs. Suppressing FOXM1 expression could promote osteoclast differentiation as well as RANKL/OPG in human PDLCs. FOXM1 also plays a role in controlling PDLC differentiation and proliferation capacity.

scholarly journals RNA sequencing data of human periodontal ligament cells treated with continuous and intermittent compressive force

Data in Brief ◽  
2019 ◽  
Vol 26 ◽  
pp. 104553
Author(s):  
Jeeranan Manokawinchoke ◽  
Prasit Pavasant ◽  
Chenphop Sawangmake ◽  
Nuttapol Limjeerajarus ◽  
Chalida N. Limjeerajarus ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Periodontal Ligament ◽  
Compressive Force ◽  
Periodontal Ligament Cells ◽  
Sequencing Data ◽  
Human Periodontal Ligament Cells

scholarly journals Compression and hypoxia play independent roles while having combinative effects in the osteoclastogenesis induced by periodontal ligament cells

2015 ◽  
Vol 86 (1) ◽  
pp. 66-73 ◽  
Author(s):  
Mei Le Li ◽  
Jianru Yi ◽  
Yan Yang ◽  
Xuan Zhang ◽  
Wei Zheng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Periodontal Ligament ◽  
Tooth Movement ◽  
Thin Sheet ◽  
Orthodontic Tooth Movement ◽  
Compressive Force ◽  
Periodontal Ligament Cells ◽  
Tartrate Resistant Acid Phosphatase ◽  
Hypoxia Group ◽  
Polymerase Chain

ABSTRACT Objective:  To investigate the isolated and combined effects of compression and hypoxia on the osteoclastogenesis induced by periodontal ligament cells (PDLCs). Materials and Methods:  A periodontal ligament tissue model (PDLtm) was established by 3-D culturing human PDLCs on a thin sheet of poly lactic-co-glycolic acid scaffold. The PDLtm was treated with hypoxia and/or compression for 6, 24, or 72 hours. After that, a real-time polymerase chain reaction was used for gene expression analysis. The conditioned media were used for the coculture of osteoblast and osteoclast (OC) precursors; tartrate-resistant acid phosphatase staining was done to examine OC formation. Results:  Either compression or hypoxia alone significantly up-regulated the gene expression of pro-osteoclastogenic cytokines in the PDLtm and enhanced osteoclastogenesis in the cocultures, and the combination of the two had significantly stronger effects than either stimulation alone. In addition, comparing the two stimulants, we found that the osteoclastogenic property of the PDLCs peaked earlier (at 6 hours) in the compression group than in the hypoxia group (at 24 hours). Conclusions:  Both compressive force and hypoxia may take part in initiating osteoclastogenesis in orthodontic tooth movement and may have combinatory effects, which could update our concepts of the mechanisms involved in the initiation of bone resorption on the pressure side of the tooth in question.

Gene expression profiling of Jagged1‐treated human periodontal ligament cells

Oral Diseases ◽  
2019 ◽  
Vol 25 (4) ◽  
pp. 1203-1213 ◽  
Author(s):  
Jaijam Suwanwela ◽  
Kanokporn Hansamuit ◽  
Jeeranan Manokawinchoke ◽  
Noppadol Sa‐Ard‐Iam ◽  
Rangsini Mahanonda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells
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