Role of mammary serine protease inhibitor on the inflammatory response in oral lichen planus

AbstractThe Serine Protease Inhibitor (serpin) protein has been suggested to play a key role in the interaction of bifidobacteria with the host. By inhibiting intestinal serine proteases, it might allow bifidobacteria to reside in specific gut niches. In inflammatory diseases where serine proteases contribute to the innate defense mechanism of the host, serpin may dampen the damaging effects of inflammation. In view of the beneficial roles of this protein, it is important to understand how its production is regulated. Here we demonstrate that Bifidobacterium longum NCC 2705 serpin production is tightly regulated by carbohydrates. Galactose and fructose increase the production of this protein while glucose prevents it, suggesting the involvement of catabolite repression. We identified that di- and oligosaccharides containing galactose (GOS) and fructose (FOS) moieties, including the human milk oligosaccharide Lacto-N-tetraose (LNT), are able to activate serpin production. Moreover, we show that the carbohydrate mediated regulation is conserved within B. longum subsp. longum strains but not in other bifidobacterial taxons harboring the serpin coding gene, highlighting that the serpin regulation circuits are not only species- but also subspecies- specific. Our work demonstrates that environmental conditions can modulate expression of an important effector molecule of B. longum, having potential important implications for probiotic manufacturing and supporting the postulated role of serpin in the ability of bifidobacteria to colonize the intestinal tract.

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Role of mast cells in pathogenesis of oral lichen planus

Journal of Oral and Maxillofacial Pathology ◽

10.4103/0973-029x.86674 ◽

2011 ◽

Vol 15 (3) ◽

pp. 267 ◽

Cited By ~ 9

Author(s):

Rachna Sharma ◽

Keya Sircar ◽

Sanjeet Singh ◽

Varun Rastogi

Keyword(s):

Mast Cells ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Oral Lichen

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Role of apoptosis in erosive and reticular oral lichen planus exhibiting variable epithelial thickness

Brazilian Dental Journal ◽

10.1590/s0103-64402008000300001 ◽

2008 ◽

Vol 19 (3) ◽

pp. 179-185 ◽

Cited By ~ 21

Author(s):

Juliana M. Caldeira Brant ◽

Anilton C. Vasconcelos ◽

Luciana V. Rodrigues

Keyword(s):

Lichen Planus ◽

Oral Mucosa ◽

Oral Lichen Planus ◽

Chronic Inflammatory Disease ◽

Mechanical Trauma ◽

Epithelial Thickness ◽

Healthy Mucosa ◽

Toxic Agents ◽

Oral Lichen

Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical types. Reticular and erosive forms are the most common. Although the cause of OLP remains speculative, many findings suggest auto-immune involvement, mediated by T lymphocytes against the basal keratinocytes. Inflammation, mechanical trauma or toxic agents can affect the epithelial homeostasia. Increased apoptosis may cause a decrease in epithelial thickness reflecting in the activity of the lesion. The objective of this study was to evaluate the occurrence of apoptosis and epithelial thickness in reticular and erosive forms of OLP. 15 samples of OLP each type (reticular and erosive) plus 10 of healthy mucosa were collected and processed. After morphometry, the apoptotic index and epitelial thickness were obtained. TUNEL and M30 CytoDEATH immunohistochemical assay were used to validate the morphologic criteria used. Apoptosis in the erosive OLP was significantly more intense than in the reticular type and both forms of OLP presented more apoptosis than the healthy oral mucosa. Healthy oral mucosa was thicker than both OLP forms and thicker in OLP reticular form than in the erosive one. The clinical differences between reticular and erosive forms of OLP are related to variations in epithelial thickness and in intensity of apoptosis.

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A Model to Evaluate Toxic Factors Influencing Islets during Collagenase Digestion: The Role of Serine Protease Inhibitor in the Protection of Islets

Cell Transplantation ◽

10.3727/096368911x605385 ◽

2012 ◽

Vol 21 (2-3) ◽

pp. 473-482 ◽

Cited By ~ 8

Author(s):

Manabu Tsukada ◽

Takuro Saito ◽

Kazuya Ise ◽

Akira Kenjo ◽

Takashi Kimura ◽

...

Keyword(s):

Protease Inhibitor ◽

Serine Protease ◽

Serine Protease Inhibitor ◽

Factors Influencing ◽

Collagenase Digestion ◽

Toxic Factors

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Salivary and Serum Interferon-Gamma/Interleukin-4 Ratio in Oral Lichen Planus Patients: A Systematic Review and Meta-Analysis

Medicina ◽

10.3390/medicina55060257 ◽

2019 ◽

Vol 55 (6) ◽

pp. 257

Author(s):

Hamid Reza Mozaffari ◽

Maryam Molavi ◽

Pia Lopez-Jornet ◽

Masoud Sadeghi ◽

Mohsen Safaei ◽

...

Keyword(s):

Lichen Planus ◽

Interferon Gamma ◽

Oral Lichen Planus ◽

Meta Analysis ◽

Interleukin 4 ◽

Cochrane Library ◽

Pathological Effect ◽

Ifn Γ ◽

Oral Lichen

Background and Objectives: Interferon-gamma (IFN-γ)/interleukin-4 (IL-4) ratio may indicate a change in the immune response with a potential pathological effect presented in oral lichen planus (OLP) patients. Herein, this meta-analysis evaluated the role of serum and salivary interferon-gamma/interleukin-4 ratio in the severity and development of OLP. Materials and Methods: The Scopus, Cochrane Library, PubMed, and Web of Science databases were systematically searched to retrieve the relevant studies published up from the database inception to March 2019. The crude mean difference (MD) and 95% confidence interval (CI) were calculated by RevMan 5.3 software using a random-effects model. A sensitivity analysis was performed on the results using the CMA 2.0 software. A total of 98 studies were retrieved from the databases, of which at last seven studies were included in this meta-analysis. Results: The findings showed that the pooled MDs of serum and salivary IFN-γ/IL-4 ratio were −0.22 (95% CI: −1.16, 0.72; p = 0.64) and 0.17 (95% CI: −1.50, 1.84; p = 0.84) in OLP patients compared to controls, respectively. In addition, the pooled MDs of serum and salivary IFN-γ/IL-4 ratio were −0.15 (95% CI: −0.53, 0.23; p = 0.43) and −0.39 (95% CI: −0.63, −0.15; p = 0.001) in patients with erythematous/ulcerative subtype compared to patients with reticular subtype, respectively. Conclusions: In conclusion, the results of meta-analysis demonstrated that serum and salivary IFN-γ/IL-4 ratio cannot play a major role in OLP development and severity.

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