To analyze the root-soil water relationship at the stand level, we integrated ground-penetrating radar (GPR), which characterized the distribution of lateral coarse roots (>2 mm in diameter) of shrubs (Caragana microphylla Lam.), with soil core sampling, which mapped soil water content (SWC) distribution. GPR surveys and soil sampling were carried out in two plots (Plot 1 in 2017 and Plot 2 in 2018) with the same size (30 × 30 m2) in the sandy soil of the semi-arid shrubland in northern China. First, the survey area was divided into five depth intervals, i.e., 0–20, 20–40, 40–60, 60–80, and 80–100 cm. Each depth interval was then divided into three zones in the horizontal direction, including root-rich canopy-covered area, root-rich canopy-free area, and root-poor area, to indicate different surface distances to the canopy. The generalized additive models (GAMs) were used to analyze the correlation between root distribution density and SWC after the spatial autocorrelation of each variable was eliminated. Results showed that the root-soil water relationship varies between the vertical and horizontal directions. Vertically, more roots are distributed in soil with high SWC and fewer roots in soil with low SWC. Namely, root distribution density is positively correlated with SWC in the vertical direction. Horizontally, the root-soil water relationship is, however, more complex. In the canopy-free area of Plot 1, the root-soil water relationship was significant (p < 0.05) and negatively correlated in the middle two depth intervals (20–40 cm and 40–60 cm). In the same two depth intervals in the canopy-free area of Plot 2, the root-soil water relationship was also significant (p < 0.01) but non-monotonic correlated, that is, with the root distribution density increasing, the mean SWC decreased first and then increased. Moreover, we discussed possible mechanisms, e.g., root water uptake, 3D root distribution, preferential flow along roots, and different growing stages, which might lead to the spatially anisotropic relationship between root distribution and SWC at the stand level. This study demonstrates the advantages of GPR in ecohydrology studies at the field scale that is challenging for traditional methods. Results reported here complement existing knowledge about the root-soil water relationship in semi-arid environments and shed new insights on modeling the complex ecohydrological processes in the root zone.
Focusing on individual plants to understand community scale biodiversity effects: the case of root distribution in grasslands
