scholarly journals Functions of Exogenous RUNX2 in Giant Cell Tumor of Bone In Vitro

2020 ◽  
Vol 12 (2) ◽  
pp. 668-678 ◽  
Author(s):  
Yuan‐han Jin ◽  
Jing Zhang ◽  
Hao Zhu ◽  
Gen‐tao Fan ◽  
Guang‐xin Zhou
Keyword(s):  
Giant Cell Tumor ◽  
Giant Cell ◽  
Cell Tumor

Giant Cell Tumor of Bone: A Model for the in Vitro Human Osteoclast Characterization

1989 ◽  
Vol 75 (4) ◽  
pp. 389-395 ◽  
Author(s):  
Mario Campanacci ◽  
Gian Paolo Bagnara ◽  
Massimo Serra ◽  
Marco Giovannini ◽  
Paolo Tornasi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Giant Cells ◽  
Cell Tumor ◽  
Cell Populations ◽  
In Vitro Growth ◽  
Human Osteoclast ◽  
High Doses

The in vitro growth pattern of cells obtained from bioptic material of ten patients with giant cell tumor of bone (GCT) was investigated. Cytochemical reactions and monoclonal antibodies raised against macrophage markers were tested on the two histologically identifiable GCT cell populations. Only monoclonal antibody EBM/11 stained both mononuclear and giant cells. EBM/11 positivity and resistance of acid phosphatase to high doses of tartrate strongly suggest that both mononuclear and giant cells belong to the same lineage.

scholarly journals The Role of TWIST in Angiogenesis and Cell Migration in Giant Cell Tumor of Bone

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Shalini Singh ◽  
Isabella W. Y. Mak ◽  
Divya Handa ◽  
Michelle Ghert
Keyword(s):  
Cell Migration ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Stromal Cells ◽  
Epithelial Mesenchymal Transition ◽  
Cell Tumor ◽  
Stable Cell Line ◽  
Mesenchymal Transition

Giant cell tumor of bone (GCT) is a bone tumor consisting of numerous multinucleated osteoclastic giant cells involved in bone resorption and neoplastic osteoblast-like stromal cells responsible for tumor growth. The tumor occasionally metastasizes to the lung; however, factors leading to metastasis in this tumor are unknown. The TWIST-1 protein (also referred to as TWIST) has been suggested to be involved in epithelial-mesenchymal transition (EMT) and tumor progression in some cancers. In this study we investigated the functional role of TWIST in GCT cell angiogenesis and migration. Overexpression of TWIST in neoplastic GCT stromal cells significantly increased mRNA and protein expression of VEGF and VEGFR1 in vitro, whereas knockdown of TWIST resulted in decreased VEGF and VEGFR1 expression. A stable cell line with TWIST overexpression resulted in features of EMT including increased cell migration and downregulation of E-cadherin. The results of our study indicate that TWIST may play an important role in angiogenesis and cell migration in GCT.

scholarly journals New Target for Precision Medicine Treatment of Giant-Cell Tumor of Bone: Sunitinib Is Effective in the Treatment of Neoplastic Stromal Cells with Activated PDGFRβ Signaling

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3543
Author(s):  
Michal Mahdal ◽  
Jakub Neradil ◽  
Peter Mudry ◽  
Silvia Paukovcekova ◽  
Iva Staniczkova Zambo ◽  
...  
Keyword(s):  
Growth Factor ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Stromal Cells ◽  
Growth Factor Receptor ◽  
Metastatic Potential ◽  
Giant Cells ◽  
Cell Tumor ◽  
Primary Bone Tumor

Giant-cell tumor of bone (GCTB) is an intermediate type of primary bone tumor characterized by locally aggressive growth with metastatic potential. The aim of this study was to identify new druggable targets among the cell signaling molecules involved in GCTB tumorigenesis. Profiles of activated signaling proteins in fresh-frozen tumor samples and tumor-derived cell lines were determined using phosphoprotein arrays. Analysis of the obtained data revealed epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor beta (PDGFRβ) as potential targets, but only the PDGFR inhibitor sunitinib caused a considerable decrease in stromal cell viability in vitro. Furthermore, in the case of a 17-year-old patient suffering from GCTB, we showed that the addition of sunitinib to the standard treatment of GCTB with the monoclonal antibody denosumab resulted in the complete depletion of multinucleated giant cells and mononuclear stromal cells in the tumor tissue. To summarize, the obtained data showed that a specific receptor tyrosine kinase (RTK) signaling pattern is activated in GCTB cells and plays an important role in the regulation of cell proliferation. Thus, activated RTKs and their downstream signaling pathways represent useful targets for precision treatment with low-molecular-weight inhibitors or with other types of modern biological therapy.

Hydrogen Peroxide Inhibits Giant Cell Tumor and Osteoblast Metabolism In Vitro

1998 ◽  
Vol 347 ◽  
pp. 250???260 ◽  
Author(s):  
Natalie C. Nicholson ◽  
Warren K. Ramp ◽  
Jeffrey S. Kneisl ◽  
Kathleen K. Kaysinger
Keyword(s):  
Hydrogen Peroxide ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Cell Tumor

Influence of Surface Chemistry on the Biological Feature of Giant Cell Tumor of Bone Stromal Cells In Vitro

2013 ◽  
Vol 3 (5) ◽  
pp. 554-563 ◽  
Author(s):  
Li-Hua Li ◽  
Ying-Hu Deng ◽  
Jin He ◽  
Guo-Bo Lan ◽  
Mei Li ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Stromal Cells ◽  
Cell Tumor ◽  
Biological Feature

In Vitro Study of the Effects of Denosumab on Giant Cell Tumor of Bone: Comparison with Zoledronic Acid

2017 ◽  
Vol 25 (1) ◽  
pp. 409-419 ◽  
Author(s):  
Isao Shibuya ◽  
Masamichi Takami ◽  
Arei Miyamoto ◽  
Akiko Karakawa ◽  
Akira Dezawa ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
In Vitro Study ◽  
Cell Tumor ◽  
Vitro Study

Extracellular Ca2+ sensing is modulated by pH in human osteoclast-like cells in vitro

1994 ◽  
Vol 267 (4) ◽  
pp. C961-C968 ◽  
Author(s):  
M. Grano ◽  
R. Faccio ◽  
S. Colucci ◽  
R. Paniccia ◽  
N. Baldini ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Apical Membrane ◽  
Cell Tumor ◽  
Polarized Cells ◽  
Human Osteoclast ◽  
Intracellular Signal ◽  
Isolated Rat

Osteoclasts are polarized cells with a basolateral and an apical membrane exposed to different extracellular Ca2+ ([Ca2+]o) and H+ (pHe) concentrations. Osteoclast bone resorption is inhibited in vitro by increases of [Ca2+]o slightly above physiological levels, detected by a [Ca2+]o sensing causing elevations of the intracellular signal, [Ca2+]i. Nevertheless, during bone resorption the apical membrane is exposed to [Ca2+]o severalfold higher than physiological without apparent inhibition of osteoclast functions. Because pHe facing the apical membrane is acidic, in this single-cell [Ca2+]i and intracellular pH study we addressed the question of whether the responses of human osteoclast-like cells from a giant cell tumor of bone to elevated [Ca2+]o are altered by reducing pHe. We first observed that low pHe stimulated Ca2+ efflux and cell acidification. We then demonstrated that the amplitude of the [Ca2+]o-dependent [Ca2+]i "spikes" is downregulated by low pHe, with approximately 70-fold higher [Ca2+]o required to induce significant responses at pHe 6.0 compared with pHe 7.4. Similar downregulation was observed in authentic freshly isolated rat osteoclasts. Finally, we observed that occupancy of the [Ca2+]o sensing by Ca2+ prompted rapid and transient cell acidification partially counteracted by a Na(+)-dependent amiloride derivative-sensitive H+ transport. These results demonstrate that the cascade of events triggered by activation of the [Ca2+]o sensing is affected by environmental pH and in turn influences cellular H+ transport. Such pH-related features of the [Ca2+] o sensing mechanism might be relevant for the regulation of osteoclast-like function.

Giant cell tumor of the tendon sheath involving skin

1978 ◽  
Vol 114 (6) ◽  
pp. 944-946 ◽  
Author(s):  
D. T. King
Keyword(s):  
Giant Cell Tumor ◽  
Giant Cell ◽  
Tendon Sheath ◽  
Cell Tumor
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