scholarly journals BIN2 negatively regulates plant defense against Verticillium dahliae in Arabidopsis and cotton

2021 ◽  
Author(s):  
Yun Song ◽  
Yaohua Zhai ◽  
Linxuan Li ◽  
Zhaoen Yang ◽  
Xiaoyang Ge ◽  
...  
Keyword(s):  
Plant Defense ◽  
Verticillium Dahliae

Cotton ACAULIS5 is involved in stem elongation and the plant defense response to Verticillium dahliae through thermospermine alteration

2015 ◽  
Vol 34 (11) ◽  
pp. 1975-1985 ◽  
Author(s):  
Huijuan Mo ◽  
Xingfen Wang ◽  
Yan Zhang ◽  
Jun Yang ◽  
Zhiying Ma
Keyword(s):  
Plant Defense ◽  
Verticillium Dahliae ◽  
Defense Response ◽  
Stem Elongation ◽  
Plant Defense Response

scholarly journals A Verticillium dahliae Extracellular Cutinase Modulates Plant Immune Responses

2018 ◽  
Vol 31 (2) ◽  
pp. 260-273 ◽  
Author(s):  
Yue-Jing Gui ◽  
Wen-Qi Zhang ◽  
Dan-Dan Zhang ◽  
Lei Zhou ◽  
Dylan P. G. Short ◽  
...  
Keyword(s):  
Cell Death ◽  
Plant Defense ◽  
Verticillium Dahliae ◽  
Fungal Pathogens ◽  
Sequence Divergence ◽  
Defense Responses ◽  
Carbohydrate Binding ◽  
Dependent Manner ◽  
Virus Induced Gene Silencing ◽  
Plant Defense Responses

Cutinases have been implicated as important enzymes during the process of fungal infection of aerial plant organs. The function of cutinases in the disease cycle of fungal pathogens that invade plants through the roots has been less studied. Here, functional analysis of 13 cutinase (carbohydrate esterase family 5 domain–containing) genes (VdCUTs) in the highly virulent vascular wilt pathogen Verticillium dahliae Vd991 was performed. Significant sequence divergence in cutinase family members was observed in the genome of V. dahliae Vd991. Functional analyses demonstrated that only VdCUT11, as purified protein, induced cell death and triggered defense responses in Nicotiana benthamiana, cotton, and tomato plants. Virus-induced gene silencing showed that VdCUT11 induces plant defense responses in Nicotiana benthamania in a BAK1 and SOBIR-dependent manner. Furthermore, coinfiltration assays revealed that the carbohydrate-binding module family 1 protein (VdCBM1) suppressed VdCUT11-induced cell death and other defense responses in N. benthamiana. Targeted deletion of VdCUT11 in V. dahliae significantly compromised virulence on cotton plants. The cutinase VdCUT11 is an important secreted enzyme and virulence factor that elicits plant defense responses in the absence of VdCBM1.

scholarly journals The Cotton Ghplp2 Positively Regulates Plant Defense against Verticillium Dahliae by Modulating Fatty Acid Accumulation and Jasmonic Acid Signaling Pathway

2021 ◽  
Author(s):  
Yutao Zhu ◽  
Xiaoqian Hu ◽  
Yujiao Jia ◽  
Linying Gao ◽  
Yakun Pei ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Jasmonic Acid ◽  
Plant Defense ◽  
Signaling Pathway ◽  
Linolenic Acid ◽  
Verticillium Dahliae ◽  
Transgenic Arabidopsis ◽  
Cotton Plants ◽  
Ja Signaling

Abstract Patatin-like proteins (PLPs) have nonspecific lipid acyl hydrolyze (LAH) activity, which can hydrolyze membrane lipids into fatty acids and lysophospholipids. The vital role of PLPs in plant growth and abiotic stress has been well elucidated. However, the function of PLPs in plant defense response against pathogens is still poorly understood. Here, we isolated and identified a novel cotton (Gossypium hirsutum) patatin-like protein gene GhPLP2. GhPLP2 expression was induced upon treatment with pathogens Verticillium dahliae, Fusarium xysporum, and signaling molecules jasmonic acid (JA), ethylene in cotton plants. Subcellular localization revealed that GhPLP2 was localized in the cell wall and plasma membrane. GhPLP2-silenced cotton plants showed reduced resistance to V. dahliae infection, while overexpression of GhPLP2 in Arabidopsis enhanced the resistance to V. dahliae, with mild symptoms, decreased disease index, and fungal biomass. Hypersensitive response, callose deposition, and H2O2 accumulation triggered by V. dahlia elicitor were reduced in silenced cotton plants. GhPLP2-transgenic Arabidopsis had more accumulation of JA and JA synthesis precursor linoleic acid (LA, 18:2) and α-linolenic acid (ALA, 18:3) than control plants. Consistently, linoleic acid, α-linolenic acid, and jasmonic acid have decreased in GhPLP2-silenced cotton plants. Further, the gene expression of the JA signaling pathway is up-regulated in transgenic Arabidopsis and down-regulated in silenced cotton plants, respectively. These results showed that GhPLP2 is involved in plants' resistance to V. dahliae by maintaining fatty acid metabolism pools for JA biosynthesis and activation of the JA signaling pathway.

scholarly journals Disruption and Overexpression of the Gene Encoding ACC (1-Aminocyclopropane-1-Carboxylic Acid) Deaminase in Soil-Borne Fungal Pathogen Verticillium dahliae Revealed the Role of ACC as a Potential Regulator of Virulence and Plant Defense

2019 ◽  
Vol 32 (6) ◽  
pp. 639-653 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
lakovos S. Pantelides ◽  
Aliki K. Tzima ◽  
Seogchan Kang ◽  
Epaminondas J. Paplomatas ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Plant Defense ◽  
Verticillium Dahliae ◽  
Fungal Pathogen ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Gene Encoding ◽  
Pathogen Virulence ◽  
Plant Growth Promoting

It has been suggested that some microorganisms, including plant growth–promoting rhizobacteria, manipulate the level of ethylene in plants by degrading 1-aminocyclopropane-1-carboxylic acid (ACC), an ethylene precursor, into α-ketobutyrate and ammonia, using ACC deaminase (ACCd). Here, we investigated whether ACCd of Verticillium dahliae, a soil-borne fungal pathogen of many important crops, is involved in causing vascular wilt disease. Overexpression of the V. dahliae gene encoding this enzyme, labeled as ACCd, significantly increased virulence in both tomato and eggplant, while disruption of ACCd reduced virulence. Both types of mutant produced more ethylene than a wild-type (70V-WT) strain, although they significantly differed in ACC content. Overexpression strains lowered ACC levels in the roots of infected plants, while the amount of ACC in the roots of plants infected with deletion mutants increased. To test the hypothesis that ACC acts as a signal for controlling defense, roots of WT and Never-ripe (Nr) tomato plants were treated with ACC before V. dahliae inoculation. Plants pretreated with ACC displayed less severe symptoms than untreated controls. Collectively, our results suggest a novel role of ACC as a regulator of both plant defense and pathogen virulence.

scholarly journals Simultaneous Editing of Two Copies of Gh14-3-3d Confers Enhanced Transgene-Clean Plant Defense Against Verticillium dahliae in Allotetraploid Upland Cotton

2018 ◽  
Vol 9 ◽  
Author(s):  
Zhennan Zhang ◽  
Xiaoyang Ge ◽  
Xiaoli Luo ◽  
Peng Wang ◽  
Qiang Fan ◽  
...  
Keyword(s):  
Plant Defense ◽  
Verticillium Dahliae ◽  
Upland Cotton

scholarly journals Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton by Verticillium dahliae by Activating JASMONATE ZIM-DOMAIN1 Expression

2014 ◽  
Vol 166 (4) ◽  
pp. 2179-2194 ◽  
Author(s):  
Chao Li ◽  
Xin He ◽  
Xiangyin Luo ◽  
Li Xu ◽  
Linlin Liu ◽  
...  
Keyword(s):  
Plant Defense ◽  
Verticillium Dahliae

The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses

Plant Science ◽  
2017 ◽  
Vol 259 ◽  
pp. 12-23 ◽  
Author(s):  
Xiao-Xiao Cheng ◽  
Li-Hong Zhao ◽  
Steven J. Klosterman ◽  
Hong-Jie Feng ◽  
Zi-Li Feng ◽  
...  
Keyword(s):  
Plant Defense ◽  
Verticillium Dahliae ◽  
Spore Germination ◽  
Defense Responses ◽  
Plant Defense Responses

Plant defense against pathogens

AccessScience ◽  
2015 ◽  
Keyword(s):  
Plant Defense
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