scholarly journals Thinopyrum intermedium TiAP1 interacts with a chitin deacetylase from Blumeria graminis f. sp. tritici and increases the resistance to Bgt in wheat

2021 ◽  
Author(s):  
Yanlin Yang ◽  
Pan Fan ◽  
Jingxia Liu ◽  
Wenjun Xie ◽  
Na Liu ◽  
...  
Keyword(s):  
Blumeria Graminis ◽  
Thinopyrum Intermedium ◽  
Chitin Deacetylase

scholarly journals Thinopyrum intermedium TiAP1 interacts with a chitin deacetylase from Blumeria graminis f. sp. tritici and increases the resistance to Bgt in wheat

2021 ◽  
Author(s):  
Yanlin Yang ◽  
Pan Fan ◽  
Wenjun Xie ◽  
Na Liu ◽  
Zubiao Niu ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
Protein Gene ◽  
Lignin Biosynthesis ◽  
Blumeria Graminis ◽  
Thinopyrum Intermedium ◽  
Yeast Two Hybrid ◽  
Chitin Deacetylase ◽  
Acid Protein ◽  
Defence Genes ◽  
Two Hybrid

SummaryThe biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt) is a crucial factor causing reduction of global wheat production. Wild wheat relatives, e.g. Thinopyrum intermedium, is one of the wild-used parents in wheat disease-resistant breeding. From T. intermedium line, we identified the aspartic acid protein gene, TiAP1, which involved in resistance against Bgt. TiAP1 is a secreted protein that accumulates in large amounts at the infection sites of powdery mildew and extends to the intercellular space. Yeast two-hybrid showed that it interacted with the chitin deacetylase (BgtCDA1) of Bgt. Host-induced gene silencing showed BgtCDA1 promotes the invasion of Bgt. Transcriptome analysis showed the cell wall xylan metabolism, lignin biosynthesis-related, and defence genes involved in the signal transduction were upregulated in the transgenic TiAP1 wheat induced by Bgt. The TiAP1 in wheat may inactivate BgtCDA1 deacetylation function, cause chitin oligomers expose to wheat chitin receptor, then trigger the wheat immune response to inhibit the growth and penetration of Bgt, and thereby enhance the tolerance of wheat to pathogens.

scholarly journals Dissimilarity of barley powdery mildew resistances Lomerit and Heils Hanna

2011 ◽  
Vol 47 (No. 3) ◽  
pp. 95-100 ◽  
Author(s):  
A. Dreiseitl
Keyword(s):  
Powdery Mildew ◽  
Agricultural Research ◽  
Blumeria Graminis ◽  
The Other ◽  
Japanese Isolate ◽  
Agricultural Research Institute ◽  
Reaction Type ◽  
Barley Cultivars ◽  
Barley Powdery Mildew ◽  
Old Japanese

  The resistance Heils Hanna (HH) was postulated in several tens of 471 previously tested winter barley cultivars. In this paper, new tests on 29 of these cultivars are reported. Thirty-two reference isolates of Blumeria graminis f.sp. hordei held in the pathogen genebank at the Agricultural Research Institute in Kromeriz, Ltd. including a Japanese isolate and five Israeli isolates were used for response tests. However, the resistance HH conferred by the gene Mla8 and herein characterised by reaction type 0 to an old Japanese isolate known as Race I was now postulated only in four cultivars. In the other 25 cultivars another resistance, characterised by reaction type 0 to Race I and also to two Israeli isolates, was detected. In addition to the two mentioned resistances, eight known (Bw, Dr2, Ha, IM9, Ln, Lv, Ra and Sp) resistances were found in the set examined. Lomerit was the only registered cultivar tested here in which the newly detected resistance was present alone, therefore, it is recommended that this resistance be designated Lo.

Screening and analysis of differentially expressed genes from an alien addition line of wheat Thinopyrum intermedium induced by barley yellow dwarf virus infection

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1114-1121 ◽  
Author(s):  
Shu-Mei Jiang ◽  
Long Zhang ◽  
Jun Hu ◽  
Rui Shi ◽  
Guang-He Zhou ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Expressed Sequence Tag ◽  
Barley Yellow Dwarf Virus ◽  
Differentially Expressed ◽  
Addition Line ◽  
Alien Chromosome ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus ◽  
Expressed Sequence

The alien addition line TAI-27 contains a pair of chromosomes of Thinopyrum intermedium that carry resistance against barley yellow dwarf virus (BYDV). A subtractive library was constructed using the leaves of TAI-27, which were infected by Schizaphis graminum carrying the GAV strain of BYDV, and the control at the three-leaf stage. Nine differentially expressed genes were identified from 100 randomly picked clones and sequenced. Two of the nine clones were highly homologous with known genes. Of the remaining seven cDNA clones, five clones matched with known expressed sequence tag (EST) sequences from wheat and (or) barley whereas the other two clones were unknown. Five of the nine differentially expressed sequences (WTJ9, WTJ11, WTJ15, WTJ19, and WTJ32) were highly homologous (identities >94%) with ESTs from wheat or barley challenged with pathogens. These five sequences and another one (WTJ18) were also highly homologous (identities >86%) with abiotic stress induced ESTs in wheat or barley. Reverse Northern hybridization showed that seven of the nine differentially expressed cDNA sequences hybridized with cDNA of T. intermedium infected by BYDV. Three of these also hybridized with cDNA of line 3B-2 (a parent of TAI-27) infected by BYDV. The alien chromosome in TAI-27 was microdissected. The second round linker adaptor mediated PCR products of the alien chromosomal DNA were labeled with digoxygenin and used as the probe to hybridize with the nine differentially expressed genes. The analysis showed that seven differentially expressed genes were homologous with the alien chromosome of TAI-27. These seven differentially expressed sequences could be used as ESTs of the alien chromosome of TAI-27. This research laid the foundation for screening and cloning of new specific functional genes conferring resistance to BYDV and probably other pathogens.Key words: suppression subtractive hybridization (SSH), expressed sequence tag (EST), linker adaptor mediated polymerase chain reaction (LA-PCR), chromosome microdissection.

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