Ratiometric Fluorescent Sensor for Copper(II) and Phosphate Ions from Aminopyrene Derivatives

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

Download Full-text

Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

10.26434/chemrxiv.7992980 ◽

2019 ◽

Author(s):

Zacharias Thiel ◽

Pablo Rivera-Fuentes

Keyword(s):

Subcellular Localization ◽

Fluorescent Probe ◽

Single Molecule ◽

Mammalian Cells ◽

Fluorescent Sensor ◽

Biological Functions ◽

Localization Microscopy ◽

Drug Activation ◽

Nitroreductase Activity ◽

Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

Download Full-text

Ground water quality of selected areas of Punjab and Sind Provinces, Pakistan: Chemical and microbiological aspects

10.31221/osf.io/wns25 ◽

2019 ◽

Author(s):

Chem Int

Keyword(s):

Ionic Concentration ◽

Chloride Ions ◽

Potassium Ions ◽

Ammonium Ions ◽

High Concentration ◽

Phosphate Ions ◽

Sodium Magnesium ◽

Very High ◽

Microbiological Aspects

The assessment of groundwater is essential for the estimation of suitability of water for safe use. An attempt has been made to study the groundwater of selected areas of Punjab (Sheikhupura & Sahiwal) and Sindh (Sindh, Jawar Dharki and Dharki), Pakistan. The results indicate that pH, color and odor were all within limits of WHO that is pH ranges 6.5–8.5, colorless and odorless, respectively. The high values of suspended solids were observed in the Sindh-1 and Dharki samples. Microbiologically only Sahiwal and Jawar Dharki were found fit for drinking purpose. Trace metals analysis of Sheikhupura-1 and Sindh-1 showed that values do not fall within limits of WHO for Iron. The ionic concentration analysis showed that high bicarbonate (HCO3-), ions are present in the samples of Sahiwal and Dharki; Sindh-1 and Jawar Dharki samples showed very high concentration for chloride ions, all samples were satisfactory level for sulphate (SO42-), sodium, magnesium and phosphate ions except samples of Sindh-1 and Jawar Dharki. High concentration of calcium and potassium ions was observed in samples of Sindh-1, while all other samples were found fit for drinking purposes in respect of nitrate, nitrite and ammonium ions. The high concentration of Fluoride was found only in Sheikhupura-2 samples.

Download Full-text

Faculty Opinions recommendation of Near-infrared fluorescent sensor for in vivo copper imaging in a murine Wilson disease model.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13931984.15387095 ◽

2012 ◽

Author(s):

Janet Morrow

Keyword(s):

Wilson Disease ◽

Near Infrared ◽

Fluorescent Sensor ◽

Disease Model

Download Full-text

Faculty Opinions recommendation of cAMPr: A single-wavelength fluorescent sensor for cyclic AMP.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.732800686.793544098 ◽

2018 ◽

Author(s):

Ted Abel ◽

Mahesh Shetty

Keyword(s):

Cyclic Amp ◽

Fluorescent Sensor

Download Full-text

A New Fluorescent Salen-uranyl Sensor for the Sub-ppm Detection of Chemical Warfare Agents

Current Organic Chemistry ◽

10.2174/1385272824999200930150313 ◽

2020 ◽

Vol 24 (20) ◽

pp. 2378-2382

Author(s):

Andrea Pappalardo ◽

Chiara M.A. Gangemi ◽

Rosa Maria Toscano ◽

Giuseppe Trusso Sfrazzetto

Keyword(s):

Real Time ◽

Fluorescent Sensor ◽

Chemical Warfare Agents ◽

Optical Response ◽

Chemical Warfare ◽

Lethal Effects ◽

Strong Affinity ◽

Chemical Attack ◽

Fluorescence Measurements ◽

Warfare Agents

Real-time sensing of Chemical Warfare Agents (CWAs) is today a crucial topic to prevent the lethal effects of a terroristic chemical attack. For this reason, the development of efficient, selective, sensitive and reversible sensoristic devices, able to detect by optical response ppm levels of these compounds, is strongly required. Here, the synthesis of a new fluorescent sensor based on a salen-uranyl scaffold, functionalized with two bodipy moieties, and its application for the detection of sub-ppm levels of CWAs is reported. Detection properties were evaluated by fluorescence measurements and selectivity tests demonstrated the strong affinity for CWAs.

Download Full-text