scholarly journals Overexpression of METAL TOLERANCE PROTEIN8 reveals new aspects of metal transport in Arabidopsis thaliana seeds

Plant Biology ◽  
2021 ◽  
Author(s):  
S. Höller ◽  
H. Küpper ◽  
D. Brückner ◽  
J. Garrevoet ◽  
K. Spiers ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Metal Tolerance ◽  
Metal Transport

scholarly journals (288) An Arabidopsis thaliana Phytochelatin Synthase (AtPCS1) Gene Expressed in Transgenic Indian Mustard Contributes Partial Tolerance to Cd and Zn

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1080E-1081
Author(s):  
Ksenija Gasic ◽  
Schuyler S. Korban
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Arabidopsis Thaliana ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Indian Mustard ◽  
35S Promoter ◽  
Phytochelatin Synthase ◽  
Mrna Accumulation ◽  
Transgenic Lines

Phytochelatins (PCs) are heavy metal binding peptides that play important roles in sequestration and detoxification of heavy metals in plants. To develop transgenic plants with increased tolerance and/or accumulation of heavy metals from soil, an Arabidopsis thaliana FLAG–tagged AtPCS1 cDNA encoding phytochelatin synthase (PCS) under the control of a 35S promoter was expressed in Indian mustard (Brassica juncea). Four transgenic Indian mustard lines, designated pc lines, with different levels of AtPCS1 mRNA accumulation and correspondent AtPCS1 protein levels were selected and analyzed for tolerance to cadmium (Cd) and zinc (Zn). Heavy metal tolerance was assessed by measuring root length of 10-day-old seedlings grown on agar medium supplemented with different concentrations of Cd (0, 100, 150, and 200 μm CdCl2) and Zn (200, 400, 600, and 800 μm ZnCl2). All transgenic lines showed significantly longer roots when grown on a medium supplemented with 100 μm CdCl2. No significant differences were observed between transgenic lines and wild type when plants were grown on higher levels of Cd. This indicated that only partial tolerance to Cd was observed in these transgenic lines. Similarly, partial tolerance for Zn was also observed in these transgenic lines, but up to levels of 400 μm ZnCl2. Expression levels of AtPCS1 protein were not related to tolerance responses for either Cd or Zn stresses in transgenic lines.

scholarly journals Differential heavy metal tolerance of Arabidopsis halleri and Arabidopsis thaliana: a leaf slice test

2003 ◽  
Vol 158 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Misuk Cho ◽  
Agnes N. Chardonnens ◽  
Karl-Josef Dietz
Keyword(s):  
Heavy Metal ◽  
Arabidopsis Thaliana ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Arabidopsis Halleri

scholarly journals Cucumber metal transport protein MTP8 confers increased tolerance to manganese when expressed in yeast and Arabidopsis thaliana

2014 ◽  
Vol 65 (18) ◽  
pp. 5367-5384 ◽  
Author(s):  
Magdalena Migocka ◽  
Anna Papierniak ◽  
Ewa Maciaszczyk-Dziubińska ◽  
Piotr Poździk ◽  
Ewelina Posyniak ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Metal Transport ◽  
Transport Protein

scholarly journals Real‐time whole‐plant dynamics of heavy metal transport in Arabidopsis halleri and Arabidopsis thaliana by gamma‐ray imaging

Plant Direct ◽  
2019 ◽  
Vol 3 (4) ◽  
Author(s):  
Kaisa Kajala ◽  
Katherine L. Walker ◽  
Gregory S. Mitchell ◽  
Ute Krämer ◽  
Simon R. Cherry ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Arabidopsis Thaliana ◽  
Real Time ◽  
Gamma Ray ◽  
Metal Transport ◽  
Arabidopsis Halleri ◽  
Whole Plant ◽  
Plant Dynamics ◽  
Gamma Ray Imaging ◽  
Heavy Metal Transport

scholarly journals Metal transport protein 8 in Camellia sinensis confers superior manganese tolerance when expressed in yeast and Arabidopsis thaliana

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Qinghui Li ◽  
Yue Li ◽  
Xiayuan Wu ◽  
Lin Zhou ◽  
Xujun Zhu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Camellia Sinensis ◽  
Metal Transport ◽  
Transport Protein ◽  
Manganese Tolerance

scholarly journals Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator Noccaea caerulescens Population Monte Prinzera in Arabidopsis thaliana Perturbs Fe, Mn, and Ni Accumulation

2021 ◽  
Vol 22 (21) ◽  
pp. 11896
Author(s):  
Elisa Fasani ◽  
Giovanni DalCorso ◽  
Gianluca Zorzi ◽  
Caterina Agrimonti ◽  
Rosaria Fragni ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Metal Tolerance ◽  
Toxic Metal ◽  
Metal Compounds ◽  
Over Expression ◽  
Thlaspi Arvense ◽  
Noccaea Caerulescens ◽  
Transgenic Lines ◽  
Ni Accumulation ◽  
Iron And Manganese

Metalliferous soils are characterized by a high content of metal compounds that can hamper plant growth. The pseudometallophyte Noccaea caerulescens is able to grow on metalliferous substrates by implementing both tolerance and accumulation of usually toxic metal ions. Expression of particular transmembrane transporter proteins (e.g., members of the ZIP and NRAMP families) leads to metal tolerance and accumulation, and its comparison between hyperaccumulator N. caerulescens with non-accumulator relatives Arabidopsis thaliana and Thlaspi arvense has deepened our knowledge on mechanisms adopted by plants to survive in metalliferous soils. In this work, two transporters, ZNT1 and NRAMP4, expressed in a serpentinic population of N. caerulescens identified on the Monte Prinzera (Italy) are considered, and their expression has been induced in yeast and in A. thaliana. In the latter, single transgenic lines were crossed to test the effect of the combined over-expression of the two transporters. An enhanced iron and manganese translocation towards the shoot was induced by overexpression of NcZNT1. The combined overexpression of NcZNT1 and NcNRAMP4 did perturb the metal accumulation in plants.

scholarly journals A Novel Histidine-Rich CPx-ATPase from the Filamentous Cyanobacterium Oscillatoria brevis Related to Multiple-Heavy-Metal Cotolerance

2002 ◽  
Vol 184 (18) ◽  
pp. 5027-5035 ◽  
Author(s):  
Liu Tong ◽  
Susumu Nakashima ◽  
Mineo Shibasaka ◽  
Maki Katsuhara ◽  
Kunihiro Kasamo
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Metal Transport ◽  
Northern Blotting ◽  
Filamentous Cyanobacterium ◽  
Sequence Element ◽  
Heavy Metal Transport

ABSTRACT A novel gene related to heavy-metal transport was cloned and identified from the filamentous cyanobacterium Oscillatoria brevis. Sequence analysis of the gene (the Bxa1 gene) showed that its product possessed high homology with heavy-metal transport CPx-ATPases. The CPC motif, which is proposed to form putative cation transduction channel, was found in the sixth transmembrane helix. However, instead of the CXXC motif that is present in the N termini of most metal transport CPx-ATPases, Bxa1 contains a unique Cys-Cys (CC) sequence element and histidine-rich motifs as a putative metal binding site. Northern blotting and real-time quantitative reverse transcription-PCR showed that expression of Bxa1 mRNA was induced in vivo by both monovalent (Cu+ and Ag+) and divalent (Zn2+ and Cd2+) heavy-metal ions at similar levels. Experiments on heavy-metal tolerance in Escherichia coli with recombinant Bxa1 demonstrated that Bxa1 conferred resistance to both monovalent and divalent heavy metals. This is the first report of a CPx-ATPase responsive to both monovalent and divalent heavy metals.

scholarly journals Pear metal transport protein PbMTP8.1 confers manganese tolerance when expressed in yeast and Arabidopsis thaliana

2021 ◽  
Vol 208 ◽  
pp. 111687
Author(s):  
Jiyu Li ◽  
Lu Zheng ◽  
Ye Fan ◽  
Yuerong Wang ◽  
Yurou Ma ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Metal Transport ◽  
Transport Protein ◽  
Manganese Tolerance
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