Comparative analyses of H2 photoproduction in magnesium- and sulfur-starved Chlamydomonas reinhardtii cultures

2017 ◽  
Vol 161 (1) ◽  
pp. 124-137 ◽  
Author(s):  
Alena A. Volgusheva ◽  
Martina Jokel ◽  
Yagut Allahverdiyeva ◽  
Galina P. Kukarskikh ◽  
Eugeni P. Lukashev ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Comparative Analyses ◽  
H2 Photoproduction

scholarly journals A Stepwise NaHSO3 Addition Mode Greatly Improves H2 Photoproduction in Chlamydomonas reinhardtii

2018 ◽  
Vol 9 ◽  
Author(s):  
Lanzhen Wei ◽  
Xin Li ◽  
Baoqiang Fan ◽  
Zhaoxing Ran ◽  
Weimin Ma
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
H2 Photoproduction

Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii

2005 ◽  
Vol 33 (1) ◽  
pp. 102-104 ◽  
Author(s):  
M.C. Posewitz ◽  
P.W. King ◽  
S.L. Smolinski ◽  
R. Davis Smith ◽  
A.R. Ginley ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Transcriptional Response ◽  
H2 Production ◽  
Hydrogenase Activity ◽  
Wild Type ◽  
Anaerobic Conditions ◽  
H2 Photoproduction ◽  
H2 Sensor

The eukaryotic green alga, Chlamydomonas reinhardtii, produces H2 under anaerobic conditions, in a reaction catalysed by an [FeFe]-hydrogenase. To identify genes that influence H2 production in C. reinhardtii, a library of 6000 colonies on agar plates was screened with sensitive chemochromic H2-sensor films for clones defective in H2 production. Two mutants of particular interest were fully characterized. One mutant, hydEF-1, is unable to assemble an active [FeFe]-hydrogenase. This is the first reported C. reinhardtii mutant that is not capable of producing any H2. The second mutant, sta7-10, is not able to accumulate insoluble starch and has significantly lowered H2-photoproduction rates in comparison with the wild-type. In hydEF-1, anaerobiosis induces transcription of the two reported C. reinhardtii hydrogenase genes, HydA1 and HydA2, indicating a normal transcriptional response to anaerobiosis. In contrast, the transcription of both hydrogenase genes in sta7-10 is significantly attenuated.

scholarly journals A new approach for sustained and efficient H2 photoproduction by Chlamydomonas reinhardtii

2018 ◽  
Vol 11 (6) ◽  
pp. 1431-1436 ◽  
Author(s):  
Sergey Kosourov ◽  
Martina Jokel ◽  
Eva-Mari Aro ◽  
Yagut Allahverdiyeva
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Electron Flow ◽  
New Approach ◽  
Photosynthetic Electron Flow ◽  
H2 Photoproduction ◽  
Pulse Illumination

Pulse-illumination re-directs photosynthetic electron flow destined for CO2 fixation to [FeFe]-hydrogenase, thus sustaining algal H2 photoproduction for 70 hours.

Prolongation of H2 photoproduction by immobilized, sulfur-limited Chlamydomonas reinhardtii cultures

2008 ◽  
Author(s):  
T LAURINAVICHENE ◽  
S KOSOUROV ◽  
M GHIRARDI ◽  
M SEIBERT ◽  
A TSYGANKOV
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
H2 Photoproduction

Function of the chloroplastic NAD(P)H dehydrogenase Nda2 for H2 photoproduction in sulphur-deprived Chlamydomonas reinhardtii

2012 ◽  
Vol 162 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Emmanuel Mignolet ◽  
Renaud Lecler ◽  
Bart Ghysels ◽  
Claire Remacle ◽  
Fabrice Franck
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
H2 Photoproduction

scholarly journals Inhibitor studies on non-photochemical plastoquinone reduction and H2 photoproduction in Chlamydomonas reinhardtii

2005 ◽  
Vol 1708 (3) ◽  
pp. 322-332 ◽  
Author(s):  
Florence Mus ◽  
Laurent Cournac ◽  
Véronique Cardettini ◽  
Amandine Caruana ◽  
Gilles Peltier
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
H2 Photoproduction

Relationships between H2 photoproduction and different electron transport pathways in sulfur-deprived Chlamydomonas reinhardtii

2009 ◽  
Vol 34 (22) ◽  
pp. 9087-9094 ◽  
Author(s):  
Taras K. Antal ◽  
Alena A. Volgusheva ◽  
Galina P. Kukarskih ◽  
Tatyana E. Krendeleva ◽  
Andrej B. Rubin
Keyword(s):  
Electron Transport ◽  
Chlamydomonas Reinhardtii ◽  
Transport Pathways ◽  
H2 Photoproduction

Enhanced H2 photoproduction by down-regulation of ferredoxin-NADP+ reductase (FNR) in the green alga Chlamydomonas reinhardtii

2013 ◽  
Vol 38 (36) ◽  
pp. 16029-16037 ◽  
Author(s):  
Yongle Sun ◽  
Mei Chen ◽  
Haomeng Yang ◽  
Jin Zhang ◽  
Tingyun Kuang ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Down Regulation ◽  
H2 Photoproduction

Development of a Rapid Survey Method of Sampling and Analysis for Asbestos in Ambient Air

1972 ◽  
Vol 30 ◽  
pp. 630-631 ◽  
Author(s):  
R. E. Heffelfinger ◽  
C. W. Melton ◽  
D. L. Kiefer ◽  
W. M. Henry ◽  
R. J. Thompson
Keyword(s):  
Neutron Activation ◽  
Ambient Air ◽  
Survey Method ◽  
Potential Hazard ◽  
Transmission Microscopy ◽  
Comparative Analyses ◽  
Asbestos Fibers ◽  
Controlled Conditions ◽  
Unequivocal Identification ◽  
Identification And Quantification

A methodology has been developed and demonstrated which is capable of determining total amounts of asbestos fibers and fibrils in air ranging from as low as fractional nanograms per cubic meter (ng/m3) of air to several micrograms/m3. The method involves the collection of samples on an absolute filter and provides an unequivocal identification and quantification of the total asbestos contents including fibrils in the collected samples.The developed method depends on the trituration under controlled conditions to reduce the fibers to fibrils, separation of the asbestos fibrils from other collected air particulates (beneficiation), and the use of transmission microscopy for identification and quantification. Its validity has been tested by comparative analyses by neutron activation techniques. It can supply the data needed to set emissions criteria and to serve as a basis for assessing the potential hazard for asbestos pollution to the populace.

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