Class III peroxidases in cellulose deficient cultured maize cells during cell wall remodeling

Primary plant cell walls are composite extracellular structures composed of three major classes of polysaccharides (pectins, hemicelluloses, and cellulose) and of proteins. The cell wall proteins (CWPs) play multiple roles during plant development and in response to environmental stresses by remodeling the polysaccharide and protein networks and acting in signaling processes. To date, the cell wall proteome has been mostly described in flowering plants and has revealed the diversity of the CWP families. In this article, we describe the cell wall proteome of an early divergent plant, Marchantia polymorpha, a Bryophyte which belong to one of the first plant species colonizing lands. It has been possible to identify 410 different CWPs from three development stages of the haploid gametophyte and they could be classified in the same functional classes as the CWPs of flowering plants. This result underlied the ability of M. polymorpha to sustain cell wall dynamics. However, some specificities of the M. polymorpha cell wall proteome could be highlighted, in particular the importance of oxido-reductases such as class III peroxidases and polyphenol oxidases, D-mannose binding lectins, and dirigent-like proteins. These proteins families could be related to the presence of specific compounds in the M. polymorpha cell walls, like mannans or phenolics. This work paves the way for functional studies to unravel the role of CWPs during M. polymorpha development and in response to environmental cues.

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The genome of the thermoacidophilic red microalga Galdieria sulphuraria encodes a small family of secreted class III peroxidases that might be involved in cell wall modification

Planta ◽

10.1007/s00425-007-0622-z ◽

2007 ◽

Vol 227 (2) ◽

pp. 353-362 ◽

Cited By ~ 14

Author(s):

C. Oesterhelt ◽

S. Vogelbein ◽

R. P. Shrestha ◽

M. Stanke ◽

A. P. M. Weber

Keyword(s):

Cell Wall ◽

Class Iii ◽

Galdieria Sulphuraria ◽

Cell Wall Modification ◽

Small Family ◽

Class Iii Peroxidases ◽

Red Microalga

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The cell wall is crucial for cellular sensitivity to low pH: the role of class III peroxidases and ethylene in cell death in Arabidopsis thaliana roots

10.11606/t.11.2018.tde-25072018-164614 ◽

2018 ◽

Cited By ~ 2

Author(s):

Jonathas Pereira das Graças

Keyword(s):

Arabidopsis Thaliana ◽

Cell Death ◽

Cell Wall ◽

Low Ph ◽

Class Iii ◽

Cellular Sensitivity ◽

Class Iii Peroxidases

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Class III peroxidases PRX01, PRX44, and PRX73 potentially target extensins during root hair growth in Arabidopsis thaliana

10.1101/2020.02.04.932376 ◽

2020 ◽

Author(s):

Eliana Marzol ◽

Cecilia Borassi ◽

Philippe Ranocha ◽

Ariel. A. Aptekman ◽

Mauro Bringas ◽

...

Keyword(s):

Cell Wall ◽

Hair Cells ◽

Root Hair ◽

Hair Growth ◽

Water Soluble ◽

Soil Conditions ◽

Class Iii ◽

Root Hair Growth ◽

Class Iii Peroxidases ◽

Root Hair Cells

AbstractRoot hair cells are important sensors of soil conditions. Expanding several hundred times their original size, root hairs grow towards and absorb water-soluble nutrients. This rapid growth is oscillatory and is mediated by continuous remodelling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins including extensins (EXTs).Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening, mediated by oxygen radical species, or polymerization of cell wall components, including the Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown.Using genetic, biochemical, and modeling approaches, we identified and characterized three root hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. The triple mutant prx01,44,73 and the PRX44 and PRX73 overexpressors had opposite phenotypes with respect to root hair growth, peroxidase activity and ROS production with a clear impact on cell wall thickness.Modeling and docking calculations suggested that these three putative EXT-PRXs may interact with non-O-glycosylated sections of EXT peptides that reduce the Tyr-to-Tyr intra-chain distances in EXT aggregates and thereby may enhance Tyr crosslinking. These results suggest that these three putative EXT-PRXs control cell wall properties during the polar expansion of root hair cells.

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