Reflectance confocal microscopy analysis of equivocal melanocytic lesions with severe regression

2017 ◽  
Vol 24 (1) ◽  
pp. 9-15 ◽  
Author(s):  
M. Agozzino ◽  
A. Ferrari ◽  
C. Cota ◽  
C. Franceschini ◽  
P. Buccini ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Reflectance Confocal Microscopy ◽  
Melanocytic Lesions ◽  
Microscopy Analysis ◽  
Confocal Microscopy Analysis

scholarly journals Correlation of Dermoscopy With In Vivo Reflectance Confocal Microscopy of Streaks in Melanocytic Lesions

2007 ◽  
Vol 143 (6) ◽  
Author(s):  
Alon Scope ◽  
Melissa Gill ◽  
Cristiane Benveuto-Andrade ◽  
Allan C. Halpern ◽  
Salvador Gonzalez ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Reflectance Confocal Microscopy ◽  
Melanocytic Lesions

scholarly journals Effects of acids used in the microabrasion technique: Microhardness and confocal microscopy analysis

2015 ◽  
pp. e506-e512 ◽  
Author(s):  
NIP Pini ◽  
DANL Lima ◽  
GMB Ambrosano ◽  
WJ da Silva ◽  
FHB Aguiar ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Microscopy Analysis ◽  
Confocal Microscopy Analysis

scholarly journals Reflectance Confocal Microscopy and Features of Melanocytic Lesions

2009 ◽  
Vol 145 (10) ◽  
Author(s):  
Giovanni Pellacani ◽  
Marco Vinceti ◽  
Sara Bassoli ◽  
Ralph Braun ◽  
Salvador Gonzalez ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Reflectance Confocal Microscopy ◽  
Melanocytic Lesions

Epi-LASIK: A Confocal Microscopy Analysis of the Corneal Epithelium and Anterior Stroma

2012 ◽  
Vol 43 (4) ◽  
pp. 319-322 ◽  
Author(s):  
Manuel Ramírez ◽  
Everardo Hernández-Quintela ◽  
Ramón Naranjo-Tackman
Keyword(s):  
Confocal Microscopy ◽  
Corneal Epithelium ◽  
Microscopy Analysis ◽  
Confocal Microscopy Analysis

Corneal Chrysiasis: In Vivo Confocal Microscopy Analysis

2010 ◽  
Vol 20 (4) ◽  
pp. 776-779 ◽  
Author(s):  
Lacopo Paladini ◽  
Ugo Menchini ◽  
Rita Mencucci
Keyword(s):  
Confocal Microscopy ◽  
In Vivo Confocal Microscopy ◽  
Microscopy Analysis ◽  
Confocal Microscopy Analysis

Quantitative Confocal Microscopy Analysis as a Basis for Search and Study of Potassium Kv1.x Channel Blockers

2015 ◽  
pp. 249-254
Author(s):  
Alexey V. Feofanov ◽  
Kseniya S. Kudryashova ◽  
Oksana V. Nekrasova ◽  
Alexander A. Vassilevski ◽  
Alexey I. Kuzmenkov ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Channel Blockers ◽  
Microscopy Analysis ◽  
Confocal Microscopy Analysis

scholarly journals Modulation of hexokinase association with mitochondria analyzed with quantitative three-dimensional confocal microscopy.

1991 ◽  
Vol 112 (3) ◽  
pp. 385-395 ◽  
Author(s):  
R M Lynch ◽  
K E Fogarty ◽  
F S Fay
Keyword(s):  
Glucose Metabolism ◽  
Confocal Microscopy ◽  
Cell Metabolism ◽  
Three Dimensional ◽  
Metabolic State ◽  
Metabolic Conditions ◽  
Microscopy Analysis ◽  
Immunocytochemical Studies ◽  
Confocal Microscopy Analysis

Hexokinase isozyme I is proposed to be associated with mitochondria in vivo. Moreover, it has been suggested that this association is modulated in coordination with changes in cell metabolic state. To test these hypotheses, we analyzed the subcellular distribution of hexokinase relative to mitochondria in paraformaldehyde-fixed astrocytes using immunocytochemistry and quantitative three-dimensional confocal microscopy. Analysis of the extent of colocalization between hexokinase and mitochondria revealed that approximately 70% of cellular hexokinase is associated with mitochondria under basal metabolic conditions. In contrast to the immunocytochemical studies, between 15 to 40% of cellular hexokinase was found to be associated with mitochondria after fractionation of astrocyte cultures depending on the exact fractionation conditions. The discrepancy between fractionation studies and those based on imaging of distributions in fixed cells indicates the usefulness of using techniques that can evaluate the distributions of "cytosolic" enzymes in cells whose subcellular ultrastructure is not severely disrupted. To determine if hexokinase distribution is modulated in concert with changes in cell metabolism, the localization of hexokinase with mitochondria was evaluated after inhibition of glucose metabolism with 2-deoxyglucose. After incubation with 2-deoxyglucose there was an approximate 35% decrease in the amount of hexokinase associated with mitochondria. These findings support the hypothesis that hexokinase is bound to mitochondria in rat brain astrocytes in vivo, and that this association is sensitive to cell metabolic state.

Sign in / Sign up

Export Citation Format

Share Document