scholarly journals Rapid and simple detection of foot-and-mouth disease virus: Evaluation of a cartridge-based molecular detection system for use in basic laboratories

2017 ◽  
Vol 65 (2) ◽  
pp. 578-584 ◽  
Author(s):  
K. V. Goller ◽  
V. Dill ◽  
M. Madi ◽  
P. Martin ◽  
Y. Van der Stede ◽  
...  
Keyword(s):  
Disease Virus ◽  
Molecular Detection ◽  
Detection System ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Simple Detection

scholarly journals Development and Evaluation of a Rapid Antigen Detection and Serotyping Lateral Flow Antigen Detection System for Foot-and-Mouth Disease Virus

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0134931 ◽  
Author(s):  
Kazuki Morioka ◽  
Katsuhiko Fukai ◽  
Kazuo Yoshida ◽  
Rie Kitano ◽  
Reiko Yamazoe ◽  
...  
Keyword(s):  
Disease Virus ◽  
Detection System ◽  
Foot And Mouth Disease ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Use of in Situ Hybridization for the Detection of Foot-and-Mouth Disease Virus in Cell Culture

1989 ◽  
Vol 1 (4) ◽  
pp. 329-332 ◽  
Author(s):  
Richard F. Meyer ◽  
Corrie C. Brown ◽  
Thomas W. Molitor ◽  
Vikram N. Vakharia
Keyword(s):  
Disease Virus ◽  
Detection System ◽  
Cdna Probe ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rna Probes ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Infected Cells

Biotinylated complementary DNA (cDNA) and RNA probes were prepared from a specific and highly conserved section of the foot-and-mouth disease virus (FMDV) genome coding for the RNA-dependent RNA polymerase. Hybridization was conducted on FMDV-infected, bovine enterovirus (BEV)-infected, and noninfected swine kidney cell cultures. The detection system utilized the enzyme system streptavidin-alkaline phosphatase, the substrate phosphate, and the chromogen nitroblue tetrazolium. Intense cytoplasmic granular staining was present at 2 and 4 hr postinfection (hpi), with less staining observed at 24 hpi. The staining was specific for FMDV, as indicated by a lack of staining of noninfected cells and BEV-infected cells. With the RNA probe, positive cells were detected up to the highest viral dilution assayed, which was approximately 96 TCID50. The cDNA probe was slightly less sensitive, detecting positive cells at lo-fold lower dilutions. This technique could prove useful in the diagnosis of foot-and-mouth disease in animals or in the detection of FMDV in biologics submitted for importation.

scholarly journals Rapid Molecular Detection of Genetically Diverted Foot and Mouth Disease Virus Serotype O During the Outbreak of 2012 in Egypt

2015 ◽  
Vol 11 (3) ◽  
pp. 96-111
Author(s):  
Sahar Abd El Rahman ◽  
Bernd Hoffmann ◽  
Bernd Haas ◽  
Mohammed El Beskawy ◽  
Mayar Othman ◽  
...  
Keyword(s):  
Disease Virus ◽  
Molecular Detection ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Serotype O ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth

scholarly journals Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia

2021 ◽  
Vol Volume 12 ◽  
pp. 187-197
Author(s):  
Shazali Mohammed Awel ◽  
Getachew Mulatu Dilba ◽  
Bruk Abraha ◽  
Demeke Zewde ◽  
Bayeta Senbata Wakjira ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Disease Virus ◽  
Molecular Detection ◽  
Addis Ababa ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Central Ethiopia ◽  
Mouth Disease Virus ◽  
Foot And Mouth

High resolution surface structure of foot-and-mouth disease virus

1978 ◽  
Vol 36 (2) ◽  
pp. 376-377
Author(s):  
S. S. Breese ◽  
H. L. Bachrach
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Surface Structure ◽  
Disease Virus ◽  
Potassium Phosphate ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Physical Measurements ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Models for the structure of foot-and-mouth disease virus (FMDV) have been proposed from chemical and physical measurements (Brown, et al., 1970; Talbot and Brown, 1972; Strohmaier and Adam, 1976) and from rotational image-enhancement electron microscopy (Breese, et al., 1965). In this report we examine the surface structure of FMDV particles by high resolution electron microscopy and compare it with that of particles in which the outermost capsid protein VP3 (ca. 30, 000 daltons) has been split into smaller segments, two of which VP3a and VP3b have molecular weights of about 15, 000 daltons (Bachrach, et al., 1975).Highly purified and concentrated type A12, strain 119 FMDV (5 mg/ml) was prepared as previously described (Bachrach, et al., 1964) and stored at 4°C in 0. 2 M KC1-0. 5 M potassium phosphate buffer at pH 7. 5. For electron microscopy, 1. 0 ml samples of purified virus and trypsin-treated virus were dialyzed at 4°C against 0. 2 M NH4OAC at pH 7. 3, deposited onto carbonized formvar-coated copper screens and stained with phosphotungstic acid, pH 7. 3.

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