An epidemiological study in wild carnivores from Spanish Mediterranean ecosystems reveals association between Leishmania infantum, Babesia spp. and Hepatozoon spp. infection and new hosts for Hepatozoon martis , Hepatozoon canis and Sarcocystis spp

Abstract Occurrence of infection or exposure to Ehrlichia canis, Hepatozoon canis and Rickettsia spp. was detected in feral cats living in two fragments from Atlantic rainforest, in Natal, RN, Brazil, and in dogs living around the parks. While serum samples were collected from 155 animals (53 cats living in the parks; 29 dogs living in human homes around the parks; and 73 dogs living at an animal control center - ACC), spleen samples were collected from 20 dogs that were euthanized at ACC. Serum samples were analyzed to Rickettsia spp. and E. canis antibodies using the indirect immunofluorescence assay. Seventeen of the 102 dogs (17%) had E. canis antibodies and 13% (20/155) of all dogs and cats (i.e. 3% (3/102) of the dogs and 32% (17/53) of the cats) were seropositive for Rickettsia spp. antigens. The animals were therefore been exposed to R. amblyommatis or by a very closely related genotype. Among the 20 dog spleen samples analyzed, eight were PCR positive for E. canis and two for H. canis (GenBank accession number MG772657 and MG772658, respectively). In none of the spleen samples were obtained amplicons for Babesia spp. through PCR. This study provided the first evidence that Rickettsia of the spotted fever group is circulating among dogs and cats in Natal.

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Molecular detection of Hepatozoon canis and Babesia canis vogeli in domestic dogs from Cuiabá, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612011000300015 ◽

2011 ◽

Vol 20 (3) ◽

pp. 253-255 ◽

Cited By ~ 16

Author(s):

Mariana Granziera Spolidorio ◽

Mariana de Medeiros Torres ◽

Wilma Neres da Silva Campos ◽

Andréia Lima Tomé Melo ◽

Michelle Igarashi ◽

...

Keyword(s):

18S Rrna ◽

Molecular Detection ◽

Domestic Dogs ◽

Hepatozoon Canis ◽

Babesia Canis ◽

Mato Grosso ◽

First Time ◽

The City ◽

Babesia Spp

The objective of this study was to report for the first time infection by Hepatozoon spp. and Babesia spp. in 10 dogs from the city of Cuiabá, State of Mato Grosso, central-western Brazil. A pair of primers that amplifies a 574 bp fragment of the 18S rRNA of Hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18S rRNA for Babesia spp. were used. Six dogs were positive for Babesia spp., and 9 were positive for Hepatozoon spp. Co-infection of Babesia spp. and Hepatozoon spp. was seen in 5 dogs. Sequenced samples revealed 100% identity with B. canis vogeli, and H. canis. This is the first molecular detection of H. canis in domestic dogs from Cuiabá. Additionally, it is described for the first time the presence of B. canis vogeli circulating among dogs in Cuiabá.

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Human cutaneous leishmaniasis due to Leishmania infantum in the Sidi Bourouis focus (Northern Tunisia): epidemiological study and isoenzymatic characterization of the parasites

Acta Tropica ◽

10.1016/s0001-706x(02)00255-3 ◽

2003 ◽

Vol 85 (1) ◽

pp. 83-86 ◽

Cited By ~ 24

Author(s):

S. Belhadj ◽

F. Pratlong ◽

M. Hammami ◽

K. Kallel ◽

J.P. Dedet ◽

...

Keyword(s):

Cutaneous Leishmaniasis ◽

Epidemiological Study ◽

Leishmania Infantum ◽

Northern Tunisia ◽

Human Cutaneous Leishmaniasis

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Detección molecular y análisis filogenético de Hepatozoon canis (Eucoccidiorida: Haemogregarinidae) en perros clínicamente sanos de Bahía Blanca (Buenos Aires)

FAVE Sección Ciencias Veterinarias ◽

10.14409/favecv.v16i1.6651 ◽

2017 ◽

Vol 16 (1) ◽

pp. 46-49

Author(s):

Gabriel CICUTTIN ◽

María N. DE SALVO

Keyword(s):

Buenos Aires ◽

Hepatozoon Canis ◽

San Luis ◽

Babesia Spp ◽

Theileria Spp

Las enfermedades caninas causadas por protozoarios y transmitidas por garrapatas representan un importante problema en medicina veterinaria. El objetivo del estudio fue detectar molecularmente Hepatozoon spp., Babesia spp. y Theileria spp. en perros clínicamente sanos de distintas regiones de Argentina y analizar la diversidad genética de los hallazgos obtenidos. Se analizaron 163 muestras de ADN de sangre de perros (40 de Ciudad Autónoma de Buenos Aires; 33 de Bahía Blanca, Buenos Aires; 15 de Castelli, Chaco; 27 de Salsipuedes, Córdoba; 40 de Merlo, San Luis; y 8 de San Miguel, Corrientes). Mediante una PCR que amplifica un fragmento variable (460-540 pb) del gen ARNr 18S incluyendo la región V4 de los géneros Hepatozoon, Babesia y Theileria, el 12,1 % (4/33) de los perros de Bahía Blanca (Buenos Aires) resultaron positivos. Las secuencias obtenidas se identificaron como Hepatozoon canis y resultaron filogenéticamente similares a hallazgos en Sudamérica y en el resto del mundo. El estudio de H. canis en Argentina mediante técnicas moleculares de diagnóstico junto con el análisis filogenético resulta de suma importancia para conocer la situación de este patógeno en el país.

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Ticks and associated pathogens in camels (Camelus dromedarius) from Riyadh province, Saudi Arabia

10.21203/rs.2.19258/v3 ◽

2020 ◽

Author(s):

Abdullah Daria Alanazi ◽

Viet Linh Nguyen ◽

Mohamed Saleh Alyousif ◽

Ranju Ravindran Santhakumari Manoj ◽

Abdulaziz Saleh Alouffi ◽

...

Keyword(s):

Saudi Arabia ◽

Anaplasma Phagocytophilum ◽

Ixodid Ticks ◽

Hepatozoon Canis ◽

Hyalomma Dromedarii ◽

Health And Wellbeing ◽

Hyalomma Excavatum ◽

Prevalent Species ◽

Babesia Spp ◽

Theileria Spp

Abstract Background: Camel production in Saudi Arabia is severely affected by various diseases and by inadequate veterinary services. Ticks and tick-borne pathogens (TBPs ) affect the health and wellbeing of camels consequently diminishing their productivity and performances. In addition, camels may act as hosts for TBPs (e.g. Anaplasma phagocytophilum ) causing diseases in humans. The current study aimed to determine the prevalence of ixodid ticks and molecularly investigate the associated pathogens in camels from Saudi Arabia. Methods: Blood and tick samples were collected from camels ( n = 170) in Riyad Province of Saudi Arabia. Ticks were morphologically identified, and blood of camels were molecularly screened for apicomplexan (i.e. Babesia spp . , Theileria spp., Hepatozoon spp.) and rickettsial parasites (i.e. Ehrlichia spp. and Anaplasma spp.). Results: Of the 170 camels examined, 116 (68.2%; 95% CI: 60.9–75.1%) were infested by ticks with a mean intensity of 2.53 (95% CI: 2.4–2.6). In total of 296 ticks collected, Hyalomma dromedarii was the most prevalent (76.4%), followed by Hyalomma impeltatum (23.3%) and Hyalomma excavatum (0.3%). Of the tested animals, 13 (7.6%; 95% CI: 4.3–12.8%) scored positive to at least one TBP, with Anaplasma platys (5.3%; 95% CI: 2.7–9.9%) being the most prevalent species, followed by Anaplasma phagocytophilum , Anaplasma sp., Ehrlichia canis and Hepatozoon canis (0.6% each; 95% CI: 0.04–3.4%). None of the camels were found to be co-infected with more than one pathogen. All samples tested negative for Babesia spp. and Theileria spp. Conclusions: The present study reveals the occurrence of different tick species and tick-borne pathogens in camels from Saudi Arabia. Importantly, these camels may carry A. phagocytophilum and A. platys , representing a potential risk to humans.

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Ticks and associated pathogens in camels (Camelus dromedarius) from Riyadh province, Saudi Arabia

10.21203/rs.2.19258/v2 ◽

2020 ◽

Author(s):

Abdullah Daria Alanazi ◽

Viet Linh Nguyen ◽

Mohamed Saleh Alyousif ◽

Ranju Ravindran Santhakumari Manoj ◽

Abdulaziz Saleh Alouffi ◽

...

Keyword(s):

Saudi Arabia ◽

Anaplasma Phagocytophilum ◽

Ixodid Ticks ◽

Hepatozoon Canis ◽

Veterinary Services ◽

Hyalomma Dromedarii ◽

Hyalomma Excavatum ◽

Prevalent Species ◽

Babesia Spp ◽

Theileria Spp

Abstract Background Camel production in Saudi Arabia is severely affected by various diseases and by inadequate veterinary services. Ticks and tick-borne pathogens (TBPs) represent a major threat to the health of camels consequently affecting their productivity and performances. In addition, camels may act as hosts for TBPs (e.g. Anaplasma phagocytophilum ) causing diseases in humans. The current study aimed to determine the prevalence of ixodid ticks and molecularly investigate the associated pathogens in camels from Saudi Arabia. Methods Blood and tick samples were collected from camels (n=170) in Riyad Province of Saudi Arabia. Ticks were morphologically identified and blood of camels were molecularly screened for apicomplexan (i.e. Babesia spp ., Theileria spp., Hepatozoon spp.) and rickettsial parasites (i.e. Ehrlichia spp. and Anaplasma spp.). Results Of the 170 camels examined, 116 (68.2%; 95% CI: 60.9–75.1) were infested by ticks with a mean intensity of 2.53 (95% CI =2.4–2.6). In total of 296 ticks collected, Hyalomma dromedarii was the most prevalent (76.4%), followed by Hyalomma impeltatum (23.3%) and Hyalomma excavatum (0.3%). Of the tested animals, 13 (7.6%; 95% CI: 4.3–12.8) scored positive to at least one TBP, with Anaplasma platys (5.3%; 95% CI: 2.7–9.9) being the most prevalent species, followed by Anaplasma phagocytophilum , Anaplasma sp., Ehrlichia canis and Hepatozoon canis (0.6% each; 95% CI: 0.04–3.4). None of the camels were found to be co-infected with more than one pathogen. All samples tested negative for Babesia spp. and Theileria spp. Conclusions Current study reveals the occurrence of different tick species and tick-borne pathogens in camels from Saudi Arabia. Importantly, these camels may carry A. phagocytophilum and A. platys , representing a potential risk to humans.

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Molecular Survey of Babesia microti (Aconoidasida: Piroplasmida) in Wild Rodents in Turkey

Journal of Medical Entomology ◽

10.1093/jme/tjz084 ◽

2019 ◽

Vol 56 (6) ◽

pp. 1605-1609 ◽

Cited By ~ 1

Author(s):

Selma Usluca ◽

Bekir Celebi ◽

Djursun Karasartova ◽

A Semra Gureser ◽

Ferhat Matur ◽

...

Keyword(s):

Sequence Data ◽

18S Rrna Gene ◽

Babesia Microti ◽

Myodes Glareolus ◽

Rrna Gene ◽

Wild Rodents ◽

Sarcocystis Spp ◽

Zoonotic Parasite ◽

Reservoir Hosts ◽

Babesia Spp

Abstract Babesia microti (Aconoidasida: Piroplasmida) (Franca, 1910) is an important tick-borne zoonotic parasite with rodents serving as reservoir hosts. In the present study, 536 rodents were captured from Burdur, Bartin, Giresun, and Yozgat provinces of Turkey between the years 2010 and 2012, and blood samples were examined for the presence of Babesia spp. using conventional PCR which targeted the 18S rRNA gene. The sequence analysis of PCR amplicons was tested for B. microti as well as for Hepatozoon spp., and Sarcocystis spp. Overall, 5.8% of the rodents were positive for B. microti: 41% in Myodes glareolus, 7.7% in Chionomys roberti, and 2% in Apodemus spp., whereas no Babesia DNA was detected in Mus macedonicus and Microtus spp. Six rodents were positive for Hepatozoon spp. and one rodent was positive for Sarcocystis spp. Overall, 14.9 and 4.5% of rodents captured from Bartin and Giresun provinces, respectively, were PCR positive for B. microti, whereas none of rodents captured in Burdur and Yozgat were positive for Babesia spp. The sequence data of B. microti from rodents revealed that all sequences belonged to the zoonotic genotype. Sequences of B. microti obtained from rodents of the Bartin province were genotypically closer to European isolates, whereas those obtained from rodents of the Giresun province were closer to Russian and Mongolian isolates.

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Evidence for widespread Leishmania infantum infection among wild carnivores in L. infantum periendemic northern Spain

Preventive Veterinary Medicine ◽

10.1016/j.prevetmed.2013.12.001 ◽

2014 ◽

Vol 113 (4) ◽

pp. 430-435 ◽

Cited By ~ 29

Author(s):

L. Del Río ◽

L. Chitimia ◽

A. Cubas ◽

I. Victoriano ◽

P. De la Rúa ◽

...

Keyword(s):

Leishmania Infantum ◽

Northern Spain ◽

Wild Carnivores

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Ticks and associated pathogens in camels (Camelus dromedarius) from Riyadh province, Saudi Arabia

10.21203/rs.2.19258/v1 ◽

2019 ◽

Author(s):

Abdullah Daria Alanazi ◽

Viet Linh Nguyen ◽

Mohamed Saleh Alyousif ◽

Ranju Ravindran Santhakumari Manoj ◽

Abdulaziz Saleh Alouffi ◽

...

Keyword(s):

Saudi Arabia ◽

Anaplasma Phagocytophilum ◽

Ixodid Ticks ◽

Hepatozoon Canis ◽

Veterinary Services ◽

Hyalomma Dromedarii ◽

Hyalomma Excavatum ◽

Prevalent Species ◽

Babesia Spp ◽

Theileria Spp

Abstract Background Camel production in Saudi Arabia is severely affected by various diseases and by inadequate veterinary services. Ticks and tick-borne pathogens (TBPs) represent a major threat to the health of camels consequently affecting their productivity and performances. In addition, camels may act as hosts for TBPs (e.g. Anaplasma phagocytophilum ) causing diseases in humans. The current study aimed to determine the prevalence of ixodid ticks and molecularly investigate the associated pathogens in camels from Saudi Arabia.Methods Blood and tick samples were collected from camels (n=170) in Riyad Province of Saudi Arabia. Ticks were morphologically identified and blood of camels were molecularly screened for apicomplexan (i.e. Babesia spp ., Theileria spp., Hepatozoon spp.) and rickettsial parasites (i.e. Ehrlichia spp. and Anaplasma spp.).Results Of the 170 camels examined, 116 (68.2%; 95% CI: 60.9–75.1) were infested by ticks with a mean intensity of 2.53 (95% CI =2.4–2.6). In total of 296 ticks collected, Hyalomma dromedarii was the most prevalent (76.4%), followed by Hyalomma impeltatum (23.3%) and Hyalomma excavatum (0.3%). Of the tested animals, 13 (7.6%; 95% CI: 4.3–12.8) scored positive to at least one TBP, with Anaplasma platys (5.3%; 95% CI: 2.7–9.9) being the most prevalent species, followed by Anaplasma phagocytophilum , Anaplasma sp., Ehrlichia canis and Hepatozoon canis (0.6% each; 95% CI: 0.04–3.4). None of the camels were found to be co-infected with more than one pathogen. All samples tested negative for Babesia spp. and Theileria spp.Conclusions Current study reveals the occurrence of different tick species and tick-borne pathogens in camels from Saudi Arabia. Importantly, these camels may carry A. phagocytophilum and A. platys , representing a potential risk to humans.

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ACHADOS CLÍNICOS E PATOLÓGICOS DE UM CÃO INFECTADO SIMULTANEAMENTE POR Ehrlichia sp., Babesia sp., Hepatozoon sp. E Anaplasma sp.: RELATO DE CASO

Revista de Agroecologia no Semiárido ◽

10.35512/ras.v4i4.4562 ◽

2020 ◽

Vol 4 (4) ◽

pp. 1

Author(s):

Edinete Lúcio Pereira ◽

Clauceane De Jesus ◽

Higina Moreira Melo ◽

Rosangela Maria Nunes da Silva ◽

Almir Pereira de Souza

Keyword(s):

Hepatozoon Canis ◽

Anaplasma Platys ◽

Babesia Spp

O aumento populacional de cães vem propagando enfermidades como as hemoparasitoses, sendo Ehrlichia sp., Babesia spp., Hepatozoon canis e Anaplasma platys as mais comuns transmitidas pelo Rhipicephalus sanguineus, podendo ocorrer co-infecção. O objetivo do estudo é relatar o caso de uma cadela atendida e diagnosticada com co-infecção de Babesiose, Anaplasmose, Erliquiose e Hepatozoonose na Clínica Médica de Pequenos Animais, do Hospital Veterinário Universitário Prof. Ivon Macedo Tabosa, da UFCG, Campus de Patos-PB. O animal estava apático e com as mucosas hipocoradas. No hemograma observou-se anemia, trombocitopenia, neutrofilia madura e leucocitose; e no esfregaço sanguíneo a presença Hepatozoon sp., Babesia sp e Anaplasma platys. Na punção de medula visualizou-se Hepatozoon sp., Babesia sp. e Ehrlichia sp. Diante do diagnóstico de co-infecções hemoparasitoses, a cadela foi tratada e retornou 15 dias ao HVUIMT com melhora significativa do quadro geral. Diante do exposto nota-se a necessidade de um maior controle do Riphicephalus sanguineus (carrapato marrom) no ambiente, por ser responsável pela transmissão de diversas enfermidades, como a Erhlichia sp., Babesia sp., Hepatozoon sp. e Anaplasma platys, as quais podem aparecer associados em um mesmo animal, o que agrava ainda mais o quadro clínico do paciente.

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