scholarly journals Molecular detection of Hepatozoon canis and Babesia canis vogeli in domestic dogs from Cuiabá, Brazil

2011 ◽  
Vol 20 (3) ◽  
pp. 253-255 ◽  
Author(s):  
Mariana Granziera Spolidorio ◽  
Mariana de Medeiros Torres ◽  
Wilma Neres da Silva Campos ◽  
Andréia Lima Tomé Melo ◽  
Michelle Igarashi ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Domestic Dogs ◽  
Hepatozoon Canis ◽  
Babesia Canis ◽  
Mato Grosso ◽  
First Time ◽  
The City ◽  
Babesia Spp

The objective of this study was to report for the first time infection by Hepatozoon spp. and Babesia spp. in 10 dogs from the city of Cuiabá, State of Mato Grosso, central-western Brazil. A pair of primers that amplifies a 574 bp fragment of the 18S rRNA of Hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18S rRNA for Babesia spp. were used. Six dogs were positive for Babesia spp., and 9 were positive for Hepatozoon spp. Co-infection of Babesia spp. and Hepatozoon spp. was seen in 5 dogs. Sequenced samples revealed 100% identity with B. canis vogeli, and H. canis. This is the first molecular detection of H. canis in domestic dogs from Cuiabá. Additionally, it is described for the first time the presence of B. canis vogeli circulating among dogs in Cuiabá.

scholarly journals Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Natasha Gandolfi Miceli ◽  
Fernando Antonio Gavioli ◽  
Luiz Ricardo Gonçalves ◽  
Marcos Rogério André ◽  
Valéria Régia Franco Sousa ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Western Region ◽  
Mato Grosso ◽  
Animal Shelters ◽  
Blood Samples ◽  
Cytauxzoon Felis ◽  
The City ◽  
Veterinary Hospital

Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) forMycoplasma haemofelis, 12 (6.7%) for ‘Candidatus M. haemominutum’ and one (0.5%) for ‘Candidatus M. turicensis’. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

scholarly journals First record of Peckia (Sarcodexia) lambens (Wiedemann, 1830) (Diptera: Sarcophagidae) parasitizing Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) in Brazil

2018 ◽  
Vol 84 (0) ◽  
Author(s):  
Ronaldo Toma ◽  
Antonia Roel ◽  
Regiane Miranda
Keyword(s):  
Spodoptera Frugiperda ◽  
First Record ◽  
Mato Grosso ◽  
First Time ◽  
The City ◽  
Lepidoptera Noctuidae ◽  
Mato Grosso Do Sul

ABSTRACT: Collections of larvae of Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) carried out in the field in three localities in the city of Campo Grande, Mato Grosso do Sul, Brazil, from October 2012 to March 2014, recorded the presence of three species of Diptera parasitizing Spodoptera frugiperda: two species of Tachinidae, Achytas incertus (Macquart, 1851) and Winthemia trinitatis Thompson, 1963, and one species of Sarcophagidae, Peckia (Sarcodexia) lambens (Wiedemann, 1830). The latter is identified for the first time parasitizing S. frugiperda in Brazil.

scholarly journals Promecops claviger Hustache, 1929 (Coleoptera: Curculionidae): first register in Brazil and first host plant register

Check List ◽  
2011 ◽  
Vol 7 (5) ◽  
pp. 616
Author(s):  
Marliton Rocha Barreto ◽  
Fábio Cavalet
Keyword(s):  
Glycine Max ◽  
Host Plant ◽  
Mato Grosso ◽  
Glycine Max L ◽  
Soybean Plants ◽  
First Time ◽  
The City

For the first time in Brazil and in soybean plants (Glycine max (L.) Merr.), the weevil Promecops claviger Hustache has been identified. The incidence occurred in the city of Sinop, Mato Grosso (11°48’14,64” S, 55°34’55,93” W), in October 2010. 

Molecular Characterization and Assessment of Hematobiochemical and Oxidative Indices in Dog Naturally Infected with Babesia canis vogeli

2021 ◽  
Author(s):  
Prabhakar Shil ◽  
Jayesh B. Solanki ◽  
Niranjan Kumar ◽  
Dharmesh C. Patel ◽  
Nabanita Thakuria
Keyword(s):  
Plasma Cortisol ◽  
18S Rrna ◽  
Gene Sequence ◽  
Molecular Detection ◽  
18S Rrna Gene ◽  
Mean Value ◽  
Serum Glucose ◽  
Rrna Gene ◽  
Babesia Canis ◽  
The Mean

Background: The study was aimed at molecular detection and assessment of important biomarkers in the natural cases of canine babesiosis. Methods: Blood samples of 239 dogs were examined in PCR by targeting 18S rRNA gene. Hematobiochemical, oxidant-antioxidant and plasma cortisol parameters were estimated in the dogs on the day of presentation. Result: The 18S rRNA gene sequence showed 100% homology with Babesia canis vogeli and phylogram formed a tight cluster of B. canis vogeli originated from India/other countries. Higher prevalence rate (P less than 0.05) was noted in the PCR (7.95%) than the cytological technique (3.76%). Hemogram of infected dogs showed decrease (P less than 0.05) in the mean value of hemoglobin, RBC, WBC, HCT, whereas an increase in MCHC, lymphocytes, eosinophils, monocytes and thrombocytes. The ALT (49.29±1.53 U/L), AST (48.33±2.93 U/L), total protein (10.56±0.60 g/dL), creatinine (1.41±0.10 mg/dL) and urea (19.32±0.97 mg/dL) showed significant (P less than 0.005) increase, whereas decrease in the levels of serum glucose (82.76±2.78 mg/dL) in the infected dogs. Activity of MDA and SOD was significantly (P less than 0.01) increased (7.50±7.08 nmole/µL blood) and decreased (0.015±3.91 nmole/µL blood) in the diseased dogs, respectively. Plasma cortisol concentration was 11.10±7.84 nmol/L and 2.77±5.78 nmol/L (P less than 0.01) in the infected and uninfected dogs, respectively.

scholarly journals Ticks and associated tick-borne pathogens from dogs and red foxes from Bulgaria

2021 ◽  
Vol 24 (4) ◽  
pp. 608-613
Author(s):  
M. S. Panayotova-Pencheva ◽  
B. Vichova ◽  
V. I. Dakova ◽  
D. S. Salkova
Keyword(s):  
Climate Changes ◽  
Rhipicephalus Sanguineus ◽  
Ixodid Ticks ◽  
Domestic Dogs ◽  
Hepatozoon Canis ◽  
Tick Population ◽  
Infectious Agents ◽  
Red Foxes ◽  
Sharp Rise ◽  
Babesia Spp

Climate changes in recent years led to a sharp rise in the tick population and an increase in the number of animals and people with tick-borne infections. The domestic and wild carnivores, especially the dogs, have a huge role for the distribution of ticks in certain areas. In this study 60 ixodid ticks collected from domestic dogs and red foxes from Bulgaria have been investigated for infection with Ehrlichia canis, Hepatozoon canis, Babesia spp., and Rickettsia spp. The results showed that the dogs were infected with two tick species - Rhipicephalus sanguineus (72%) and Ixodes ricinus (28%). The red foxes were infected with only one species - I. ricinus. Out of all R. sanguineus ticks, 43.6% were female and 56.4% male. The opposite was observed for I. ricinus - female specimens (86.7%) were significantly more prevalent than males (13.3%). Similar trend was found out for I. ricinus collected from red foxes - 66.7% of the ticks were female and 33.3% male. Infectious agents were found in 31.7% of the investigated ticks. Ehrlichia spp. was established in 79% and Rickettsia spp. in 21% of the infected ticks. Ehrlichia spp. was found only in ticks collected from dogs. The majority of the ticks infected with Ehrlichia spp. were Rh. sanguineus (93.3%) and only one tick was I. ricinus (6.7%). Four ticks were positive for Rickettsia spp., two were Rh. sanguineus and two - I. ricinus, one of the latter was found on a fox. This is the first report about detection of Ehrlichia spp. in Rh. sanguineus ticks from Bulgaria as well as Rickettsia spp. in I. ricinus ticks collected from red foxes from this country.

scholarly journals Molecular Detection of Zoonotic and Non-Zoonotic Pathogens from Wild Boars and Their Ticks in the Corsican Wetlands

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1643
Author(s):  
Baptiste Defaye ◽  
Sara Moutailler ◽  
Christian Pietri ◽  
Clemence Galon ◽  
Sébastien Grech-Angelini ◽  
...  
Keyword(s):  
Wild Boar ◽  
Animal Species ◽  
Molecular Detection ◽  
Rhipicephalus Sanguineus ◽  
Animal Population ◽  
Blood Samples ◽  
Wild Boars ◽  
On This Island ◽  
First Time ◽  
Babesia Spp

Corsica is the main French island in the Mediterranean Sea and has high levels of human and animal population movement. Among the local animal species, the wild boar is highly prevalent in the Corsican landscape and in the island’s traditions. Wild boars are the most commonly hunted animals on this island, and can be responsible for the transmission and circulation of pathogens and their vectors. In this study, wild boar samples and ticks were collected in 17 municipalities near wetlands on the Corsican coast. A total of 158 hunted wild boars were sampled (523 samples). Of these samples, 113 were ticks: 96.4% were Dermacentor marginatus, and the remainder were Hyalomma marginatum, Hyalomma scupense and Rhipicephalus sanguineus s.l. Of the wild boar samples, only three blood samples were found to be positive for Babesia spp. Of the tick samples, 90 were found to be positive for tick-borne pathogens (rickettsial species). These results confirm the importance of the wild boar as a host for ticks carrying diseases such as rickettsiosis near wetlands and recreational sites. Our findings also show that the wild boar is a potential carrier of babesiosis in Corsica, a pathogen detected for the first time in wild boars on the island.

scholarly journals Incidence of Canine Tick Vectors and Molecular Detection of Haemoparasites in Vectors and Hosts

2021 ◽  
Author(s):  
A. Jena ◽  
S. Baidya ◽  
S. Pandit ◽  
R. Jas ◽  
S.C. Mandal ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Tick Infestation ◽  
Rhipicephalus Sanguineus ◽  
Hepatozoon Canis ◽  
Babesia Gibsoni ◽  
Veterinary Clinic ◽  
Primer Sets ◽  
One Year ◽  
Tick Vectors ◽  
Babesia Spp

Background: Ticks are of great importance in transmission of various canine tick borne diseases. Several characteristics of ticks make them outstanding vectors of pathogenic agents, the wide host range and slow feeding habit along with tendency to feed on several hosts during life cycle ensures ample opportunity to acquire and transmit pathogens. Methods: This study focuses on status of canine tick vectors and molecular detection of haemoparasites in these ticks and their host, in and around Kolkata. The blood and tick samples were collected from Dog Ward, Department of Teaching Veterinary and Clinical Complex; Faculty of Veterinary and Animal Sciences, WBUAFS at Belgachia; Veterinary Clinic of Kolkata Police Dog Squad at Alipore; Veterinary Clinic of Barrackpore Police dog Squad and samples from stray dogs were also collected from inside the University campus through a period of one year (August, 2016 to July, 2017). Result: The tick infestation was recorded at 41% with Rhipicephalus sanguineus, being the only tick. Nine primer sets were used for detection of Babesia spp, Ehrlichia canis, Ehrlichia chaffensis, Babesia gibsoni, Hepatozoon canis, Mycoplasma haemocanis, Anaplasma platys and Theileria annae from the respective tick samples and blood sample of hosts. Tick samples were found positive for Babesia spp, Ehrlichia spp. and H. canis where as the corresponding blood samples were found positive for Babesia spp, Ehrlichia spp., Mycoplasma spp. and H. canis. This study conclusively provides evidence of high rates of incidence of haemoparasitic infection or canine tick borne diseases infection and tick infestation, with at least four haemoparasites infecting the dog population and at least one tick species (Rhipicephalus sanguineus) infesting the dogs in and around Kolkata.

scholarly journals Hematological disorders detected in dogs infected by Hepatozoon canis in a municipality in Mato Grosso do Sul State, Brazil

2016 ◽  
Vol 68 (5) ◽  
pp. 1187-1194 ◽  
Author(s):  
L.M. Paiz ◽  
R.C. Silva ◽  
F. Satake ◽  
T.L. Fraga
Keyword(s):  
Clinical Suspicion ◽  
Hepatozoon Canis ◽  
First Year ◽  
Mato Grosso ◽  
Hematological Disorders ◽  
Blood Smears ◽  
Hematological Changes ◽  
Hematological Alterations ◽  
Mato Grosso Do Sul ◽  
Babesia Spp

ABSTRACT A retrospective review of hematological reports of nine dogs detected with Hepatozoon canis infection by microscopic examination of blood smears in a laboratory in the municipality of Dourados, Mato Grosso do Sul, Brazil was conducted. This study aimed to evaluate the hematological profile of these infected dogs, in addition to the occurrence of coinfections with other agents that infect blood cells, since studies concerning canine hepatozoonosis in Brazil are scarce and there are some divergences regarding H. canis infection that still require a resolution. The nine cases of H. canis infection were identified among all dogs examined at the studied laboratory in 2009 and 2010, with an occurrence of 7/1,192 (0.59%; 95% CI 0.15 - 1.02%) positive dogs in the first year and 2/1,313 (0.15%; 95% CI 0.02 - 0.55%) cases in 2010. The analysis of the hematological reports showed an occurrence of coinfection between H. canis and other agents in two (2/9; 22.22%; 95% CI 2.81 - 60.00%) dogs, one with E. canis and another with Babesia spp. (1/9; 11.11%; 95% CI 0.28 - 48.24%). Only the blood test of one dog had no alterations, based on reference values. Anemia was the most frequent hematological alteration (6/9; 66.67%; 95% CI 29.93 - 92.51%). Although the occurrence of H. canis infection was low, significative hematological alterations were observed in most infected dogs. Coinfection with Babesia spp. and E. canis was detected in two dogs and the hematological alterations cannot be attributed exclusively to H. canis in these animals. Longitudinal studies would be of fundamental importance to determine the causality of these alterations. These results highlight the importance of differential diagnosis in dogs when there is clinical suspicion of infection by hemoparasites, since the hematological changes in dogs infected by H. canis are quite variable.

Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys and Ehrlichia canis in dogs on Costa Rica

2014 ◽  
Vol 60 (1) ◽  
Author(s):  
Lanjing Wei ◽  
Patrick Kelly ◽  
Kate Ackerson ◽  
Heba S. El-Mahallawy ◽  
Bernhard Kaltenboeck ◽  
...  
Keyword(s):  
Costa Rica ◽  
Dirofilaria Immitis ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Hepatozoon Canis ◽  
Anaplasma Platys ◽  
Vector Borne Diseases ◽  
Tropical Areas ◽  
Vector Borne ◽  
Babesia Spp

AbstractAlthough vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas, there is little information on these conditions in Costa Rica. In PCRs of blood from dogs in Costa Rica, we did not detect DNAs of Rickettsia (R.) felis and Coxiella (C.) burnetii but we did find evidence of infection with Dirofilaria (D.) immitis (9/40, 22.5%), Hepatozoon (H.) canis (15/40, 37.5%), Babesia spp. (10/40, 25%; 2 with B. gibsoni and 8 with B. vogeli), Anaplasma (A.) platys (3/40, 7.5%) and Ehrlichia (E.) canis (20/40, 50%). Nine dogs (22.5%) were free of any vector-borne pathogens while 14 (35%) were infected with a single pathogen, 11 (27.5%) with two, 4 (10%) with three, 1 (2.5%) with four, and 1 (2.5%) with five pathogens. Dogs in Costa Rica are commonly infected with vector-borne agents

Antitumoral and Anticholinesterasic Activities of the Seven Species from Rubiaceae

2019 ◽  
Vol 20 (4) ◽  
pp. 302-308 ◽  
Author(s):  
Carla R.F. Volobuff ◽  
Pedro C.O. Junior ◽  
Sidney M. dos Santos ◽  
Zefa V. Pereira ◽  
Diego C. Ferreira ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Biological Activities ◽  
Brain Structures ◽  
Acetylcholinesterase Inhibition ◽  
Mato Grosso ◽  
Male Wistar Rats ◽  
First Time ◽  
Mato Grosso Do Sul ◽  
Antiproliferative Activities ◽  
Mcf 7

Background: The genus Psychotria and Palicourea are reported as a source of alkaloids and iridoids, which exhibit biological activities. This study aimed to evaluate antiproliferative and anticholinesterase activities and quantification of the alkaloids of seven species among the genus found in Mato Grosso do Sul region in Brazil. Methods: Concentrations of alkaloids were measured spectrophotometrically. The extracts were submitted to antiproliferative activity against ten cell lines. The anticholinesterase activity of the extracts was developed using brain structures of male Wistar rats: cerebral cortex, hippocampus, hypothalamus and striatum by the Ellman method. Results: Alkaloids from Psychotria and Palicourea species were quantified which showed values of 47.6 to 21.9 µg/g. Regarding the antiproliferative potential, Palicourea crocea demonstrated selectivity against the 786-0 cell line (GI50: 22.87 µg/mL). Psychotria leiocarpa inhibited cell growth against OVCAR-3 (GI50: 3.28 µg/mL), K-562 (GI50: 5.26 µg/mL), HaCaT (GI50: 27.20 µg/mL), PC-3 (GI50: 34.92 µg/mL), MCF-7 (GI50: 35.80 µg/mL) and P. capillacea showed activity against OVCAR-3 (GI50: 2.33 µg/ml) and U251 (GI50: 16.66 µg/ml). The effect of acetylcholinesterase inhibition was more effective in the hippocampus, demonstrating inhibition for Paliourea crocea, Psychotria deflexa, P. brachybotrya and P. leiocarpa of 70%, 57%, 50% and 40%, respectively, followed by P. poeppigiana and P. capillacea, inhibiting 21%, compared to the control. Conclusion: Herein, the present work showed for the first time, anticholinesterasic and antiproliferative activities of extracts of Palicourea and Psychotria seem to be mainly associated with the levels of alkaloids in the leaves of these species.

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