Molecular detection and characterization of African swine fever virus from field outbreaks in domestic pigs, Mizoram, India

2021 ◽  
Author(s):  
Tridib Kumar Rajkhowa ◽  
J. Kiran ◽  
Lalthapui Hauhnar ◽  
Doris Zodinpui ◽  
Amitava Paul ◽  
...  
Keyword(s):  
Molecular Detection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs

scholarly journals Molecular characterization of African swine fever virus in apparently healthy domestic pigs in Uganda

2014 ◽  
Vol 13 (25) ◽  
pp. 2491-2499 ◽  
Author(s):  
Kalenzi Atuhaire David ◽  
Ochwo Sylvester ◽  
Afayoa Mathias ◽  
Mwesigwa Savannah ◽  
Norbert Mwiine Frank ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs ◽  
Apparently Healthy

scholarly journals Molecular Detection of Torque Teno Sus Virus and Coinfection with African Swine Fever Virus in Blood Samples of Pigs from Some Slaughterhouses in Nigeria

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Pam D. Luka ◽  
Joseph Erume ◽  
Bitrus Yakubu ◽  
Olajide A. Owolodun ◽  
David Shamaki ◽  
...  
Keyword(s):  
Molecular Detection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Comparative Sequence Analysis ◽  
Fever Virus ◽  
Nucleic Acid Detection ◽  
Viral Nucleic Acid ◽  
Blood Samples ◽  
Domestic Pigs ◽  
Torque Teno Sus Virus

Torque teno sus virus 1 (TTSuV1a/TTSuV1b) infection is present in pig herds worldwide. This study investigated the prevalence of TTSuV1a/TTSuV1b infections in domestic pigs from some slaughterhouses in Nigeria as well as coinfection with African swine fever virus (ASFV) and described the phylogeny in relation to global strains. One hundred and eighty-one (181) blood samples from four slaughterhouses were used for the study and viral nucleic acid detection was carried out by PCR. Comparative sequence analysis was carried out to infer phylogeny. The overall prevalence of TTSuV1a/b was 17.7%. Prevalence of individual genotypes was 10.5% and 7.2% for TTSuV1a and TTSuV1b, respectively. Coinfection of ASFV/TTSuV1a/b was 7.7% while that of TTSuV1a and TTSuV1b was 1.7%. ASFV alone was detected in 11.91% of the total samples. The Nigerian TTSuV1a and TTSuV1b shared a sequence identity of 91–100% and 95–100%, respectively, among each other. The ASFV sequences were 100% identical to members of genotype 1. This is the first report on the presence of TTSuV1a/b in domestic pigs in Nigeria and coinfection with ASFV. Although the prevalence of TTSuV1a/b in Nigeria was low, we recommend further studies to establish the trend and possible role in the pathogenesis of ASFV.

scholarly journals Molecular characterization of African swine fever virus from domestic pigs in northern Tanzania during an outbreak in 2013

2014 ◽  
Vol 46 (7) ◽  
pp. 1199-1207 ◽  
Author(s):  
Gerald Misinzo ◽  
David E. Kwavi ◽  
Christopher D. Sikombe ◽  
Mariam Makange ◽  
Emma Peter ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs ◽  
Northern Tanzania

Genetic characterization of African swine fever virus from domestic pigs in India

2021 ◽  
Author(s):  
Katherukamem Rajukumar ◽  
Dhanapal Senthilkumar ◽  
Govindarajulu Venkatesh ◽  
Fateh Singh ◽  
Vishnu P. Patil ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs

scholarly journals Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Stephen McCleary ◽  
Rebecca Strong ◽  
Ronan R. McCarthy ◽  
Jane C. Edwards ◽  
Emma L. Howes ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs

scholarly journals Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples

Viruses ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 820 ◽  
Author(s):  
Ayushi Rai ◽  
Sarah Pruitt ◽  
Elizabeth Ramirez-Medina ◽  
Elizabeth A. Vuono ◽  
Ediane Silva ◽  
...  
Keyword(s):  
Cell Line ◽  
Virus Isolation ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Clinical Samples ◽  
Outbreak Strain ◽  
Domestic Pigs ◽  
Current Outbreak ◽  
High Degree

African swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar in Europe and Asia. In 2018, the largest pig producing country, China, reported its first outbreak of African swine fever (ASF). Since then, the disease has quickly spread to all provinces in China and to other countries in southeast Asia, and most recently to India. Outbreaks of the disease occur in Europe as far west as Poland, and one isolated outbreak has been reported in Belgium. The current outbreak strain is highly contagious and can cause a high degree of lethality in domestic pigs, leading to widespread and costly losses to the industry. Currently, detection of infectious ASFV in field clinical samples requires accessibility to primary swine macrophage cultures, which are infrequently available in most regional veterinary diagnostic laboratories. Here, we report the identification of a commercially available cell line, MA-104, as a suitable substrate for virus isolation of African swine fever virus.

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