scholarly journals The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

2021 ◽  
Author(s):  
Lei Hua ◽  
Sean R. Stevenson ◽  
Ivan Reyna‐Llorens ◽  
Haiyan Xiong ◽  
Stanislav Kopriva ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Water Transport ◽  
Active Role ◽  
Acid Synthesis ◽  
Bundle Sheath ◽  
Sulfur Assimilation

scholarly journals The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

2021 ◽  
Author(s):  
Lei Hua ◽  
Sean R. Stevenson ◽  
Ivan Reyna-Llorens ◽  
Haiyan Xiong ◽  
Stanislav Kopriva ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Comparative Analysis ◽  
Jasmonic Acid ◽  
Water Transport ◽  
Acid Synthesis ◽  
Bundle Sheath ◽  
Last Common Ancestor ◽  
Bundle Sheath Cells ◽  
Sheath Cells

Abstract Leaves comprise multiple cell types but our knowledge of the patterns of gene expression that underpin their functional specialization is fragmentary. Our understanding and ability to undertake rational redesign of these cells is therefore limited. We aimed to identify genes associated with the incompletely understood bundle sheath of C3 plants, which represents a key target associated with engineering traits such as C4 photosynthesis into rice. To better understand veins, bundle sheath and mesophyll cells of rice we used laser capture microdissection followed by deep sequencing. Gene expression of the mesophyll is conditioned to allow coenzyme metabolism and redox homeostasis as well as photosynthesis. In contrast, the bundle sheath is specialized in water transport, sulphur assimilation and jasmonic acid biosynthesis. Despite the small chloroplast compartment of bundle sheath cells, substantial photosynthesis gene expression was detected. These patterns of gene expression were not associated with presence/absence of particular transcription factors in each cell type, but rather gradients in expression across the leaf. Comparative analysis with C3Arabidopsis identified a small gene-set preferentially expressed in bundle sheath cells of both species. This included genes encoding transcription factors from fourteen orthogroups, and proteins allowing water transport, sulphate assimilation and jasmonic acid synthesis. The most parsimonious explanation for our findings is that bundle sheath cells from the last common ancestor of rice and Arabidopsis was specialized in this manner, and since the species diverged these patterns of gene expression have been maintained. Significance statement The role of bundle sheath cells in C4 species have been studied intensively but this is not the case in leaves that use the ancestral C3 pathway. Here, we show that gene expression in the bundle sheath of rice is specialized to allow sulphate and nitrate reduction, water transport and jasmonate synthesis, and comparative analysis with Arabidopsis indicates ancient roles for bundle sheath cells in water transport, sulphur and jasmonate synthesis.

scholarly journals Arabidopsis Pollen Fertility Requires the Transcription Factors CITF1 and SPL7 That Regulate Copper Delivery to Anthers and Jasmonic Acid Synthesis

2017 ◽  
Vol 29 (12) ◽  
pp. 3012-3029 ◽  
Author(s):  
Jiapei Yan ◽  
Ju-Chen Chia ◽  
Huajin Sheng ◽  
Ha-il Jung ◽  
Tetiana-Olena Zavodna ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Jasmonic Acid ◽  
Pollen Fertility ◽  
Acid Synthesis ◽  
Copper Delivery ◽  
Arabidopsis Pollen

scholarly journals 12-oxophytodienoic acid reductase 3 (OPR3) functions as NADPH-dependent α,β-ketoalkene reductase in detoxification and monodehydroascorbate reductase in redox homeostasis

2019 ◽  
Author(s):  
Daniel Maynard ◽  
Vijay Kumar ◽  
Jens Sproß ◽  
Karl-Josef Dietz
Keyword(s):  
Double Bond ◽  
Jasmonic Acid ◽  
Wide Spectrum ◽  
Redox Homeostasis ◽  
Acid Synthesis ◽  
Monodehydroascorbate Reductase ◽  
Old Yellow Enzyme ◽  
Ability To Regenerate ◽  
Double Bond Reductase

AbstractArabidopsis (Arabidopsis thaliana) 12-oxophytodienoic acid reductase isoform 3 (OPR3) is involved in the synthesis of jasmonic acid by reducing the α,β-unsaturated double bond of the cyclopentenone moiety in 12-oxo-phytodienoic acid. Recent research revealed that jasmonic acid synthesis is not strictly dependent on the peroxisomal OPR3. In addition, OPR3 is able to reduce trinitrotoluene suggesting that the old yellow enzyme homologue OPR3 has additional functions. Here we demonstrate that OPR3 catalyzes the reduction of a wide spectrum of electrophilic species that share a reactivity towards the major redox buffers glutathione (GSH) and ascorbate (ASC). Furthermore, we demonstrate that OPDA reacts with ascorbate to form an ASC-OPDA adduct, but in addition OPR3 has the ability to regenerate ASC from monodehydroascorbate (MDHA). The presented data characterize OPR3 as a bifunctional enzyme with NADPH-dependent α,β-ketoalkene double bond reductase and monodehydroascorbate reductase activities (MDHAR). opr3 mutants exhibited a slightly less reduced ASC pool in leaves in line with the MDHAR activity of OPR3 in vitro. These functions link redox homeostasis as mediated by ASC and GSH with OPR3 activity and metabolism of reactive electrophilic species (RES).

scholarly journals Jasmonic acid and ERF family genes are involved in chilling sensitivity and seed browning of pepper fruit after harvest

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jeong Gu Lee ◽  
Gibum Yi ◽  
Jieun Seo ◽  
Byoung-Cheorl Kang ◽  
Jeong Hee Choi ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Fruit Quality ◽  
Acid Synthesis ◽  
Differentially Expressed ◽  
Cold Chain ◽  
Pepper Fruit ◽  
Chilling Sensitivity ◽  
Erf Family ◽  
Branched Chain ◽  
Chilling Response

Abstract Pepper (Capsicum annuum L.) fruit is sensitive to temperatures below 10 °C, which severely diminish fruit quality during cold chain distribution. Seed browning was a major chilling symptom in 36 genotypes of C. annuum fruit screened after storage at 2 °C for 3 weeks. Among them, pepper fruits of chilling-insensitive ‘UZB-GJG-1999–51’ and -sensitive ‘C00562’ were treated at 2 °C for 0 or 24 h, respectively. Analyses of integrated transcriptome-metabolome and relative gene expression in seeds obtained from the two genotypes were conducted to identify key factors involved in the seed browning induced by chilling. The relative contents of branched-chain amino acids such as leucine, isoleucine, and valine were significantly increased after chilling. Transcriptome identification showed 3,140 differentially expressed genes (log twofold change > 1.0 and FDR-corrected p value < 0.05) affected by chilling between the two genotypes. Particularly, genes related to jasmonic acid synthesis and signaling were differentially expressed. A regulatory network of jasmonic acid synthesis and signaling, and regulation of ERF family genes might contribute to chilling response in pepper fruit. The results of this study may help facilitate further studies to develop chilling-insensitive peppers and could be a basis for improving postharvest fruit quality.

A novel stress-inducible 12-oxophytodienoate reductase from Arabidopsis thaliana provides a potential link between Brassinosteroid-action and Jasmonic-acid synthesis

2000 ◽  
Vol 157 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Carsten Müssig ◽  
Christian Biesgen ◽  
Janina Lisso ◽  
Ursula Uwer ◽  
Elmar W. Weiler ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Acid Synthesis ◽  
Potential Link

scholarly journals The Arabidopsis DELAYED DEHISCENCE1 Gene Encodes an Enzyme in the Jasmonic Acid Synthesis Pathway

2000 ◽  
Vol 12 (7) ◽  
pp. 1041-1061 ◽  
Author(s):  
Paul M. Sanders ◽  
Pei Yun Lee ◽  
Christian Biesgen ◽  
James D. Boone ◽  
Thomas P. Beals ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Acid Synthesis

The Arabidopsis DELAYED DEHISCENCE1 Gene Encodes an Enzyme in the Jasmonic Acid Synthesis Pathway

2000 ◽  
Vol 12 (7) ◽  
pp. 1041 ◽  
Author(s):  
Paul M. Sanders ◽  
Pei Yun Lee ◽  
Christian Biesgen ◽  
James D. Boone ◽  
Thomas P. Beals ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Acid Synthesis

scholarly journals Expression ofVitis amurensis NAC26in Arabidopsis enhances drought tolerance by modulating jasmonic acid synthesis

2016 ◽  
Vol 67 (9) ◽  
pp. 2829-2845 ◽  
Author(s):  
Linchuan Fang ◽  
Lingye Su ◽  
Xiaoming Sun ◽  
Xinbo Li ◽  
Mengxiang Sun ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Jasmonic Acid ◽  
Acid Synthesis

scholarly journals Root jasmonic acid synthesis and perception regulate folivore-induced shoot metabolites and increaseNicotiana attenuataresistance

2014 ◽  
Vol 202 (4) ◽  
pp. 1335-1345 ◽  
Author(s):  
Variluska Fragoso ◽  
Eva Rothe ◽  
Ian T. Baldwin ◽  
Sang-Gyu Kim
Keyword(s):  
Jasmonic Acid ◽  
Acid Synthesis
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