Arabidopsis Pollen Fertility Requires the Transcription Factors CITF1 and SPL7 That Regulate Copper Delivery to Anthers and Jasmonic Acid Synthesis

Abstract Leaves comprise multiple cell types but our knowledge of the patterns of gene expression that underpin their functional specialization is fragmentary. Our understanding and ability to undertake rational redesign of these cells is therefore limited. We aimed to identify genes associated with the incompletely understood bundle sheath of C3 plants, which represents a key target associated with engineering traits such as C4 photosynthesis into rice. To better understand veins, bundle sheath and mesophyll cells of rice we used laser capture microdissection followed by deep sequencing. Gene expression of the mesophyll is conditioned to allow coenzyme metabolism and redox homeostasis as well as photosynthesis. In contrast, the bundle sheath is specialized in water transport, sulphur assimilation and jasmonic acid biosynthesis. Despite the small chloroplast compartment of bundle sheath cells, substantial photosynthesis gene expression was detected. These patterns of gene expression were not associated with presence/absence of particular transcription factors in each cell type, but rather gradients in expression across the leaf. Comparative analysis with C3Arabidopsis identified a small gene-set preferentially expressed in bundle sheath cells of both species. This included genes encoding transcription factors from fourteen orthogroups, and proteins allowing water transport, sulphate assimilation and jasmonic acid synthesis. The most parsimonious explanation for our findings is that bundle sheath cells from the last common ancestor of rice and Arabidopsis was specialized in this manner, and since the species diverged these patterns of gene expression have been maintained. Significance statement The role of bundle sheath cells in C4 species have been studied intensively but this is not the case in leaves that use the ancestral C3 pathway. Here, we show that gene expression in the bundle sheath of rice is specialized to allow sulphate and nitrate reduction, water transport and jasmonate synthesis, and comparative analysis with Arabidopsis indicates ancient roles for bundle sheath cells in water transport, sulphur and jasmonate synthesis.

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The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

The Plant Journal ◽

10.1111/tpj.15292 ◽

2021 ◽

Author(s):

Lei Hua ◽

Sean R. Stevenson ◽

Ivan Reyna‐Llorens ◽

Haiyan Xiong ◽

Stanislav Kopriva ◽

...

Keyword(s):

Jasmonic Acid ◽

Water Transport ◽

Active Role ◽

Acid Synthesis ◽

Bundle Sheath ◽

Sulfur Assimilation

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Control of salicylic acid synthesis and systemic acquired resistance by two members of a plant-specific family of transcription factors

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1005225107 ◽

2010 ◽

Vol 107 (42) ◽

pp. 18220-18225 ◽

Cited By ~ 152

Author(s):

Y. Zhang ◽

S. Xu ◽

P. Ding ◽

D. Wang ◽

Y. T. Cheng ◽

...

Keyword(s):

Salicylic Acid ◽

Transcription Factors ◽

Systemic Acquired Resistance ◽

Acquired Resistance ◽

Acid Synthesis

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Functions of Jasmonic Acid in Plant Regulation and Response to Abiotic Stress

International Journal of Molecular Sciences ◽

10.3390/ijms21041446 ◽

2020 ◽

Vol 21 (4) ◽

pp. 1446 ◽

Cited By ~ 9

Author(s):

Jia Wang ◽

Li Song ◽

Xue Gong ◽

Jinfan Xu ◽

Minhui Li

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Jasmonic Acid ◽

Signaling Pathways ◽

Signaling Pathway ◽

Large Scale ◽

Higher Plants ◽

Complex Signal ◽

Ja Signaling ◽

Signal Network

Jasmonic acid (JA) is an endogenous growth-regulating substance, initially identified as a stress-related hormone in higher plants. Similarly, the exogenous application of JA also has a regulatory effect on plants. Abiotic stress often causes large-scale plant damage. In this review, we focus on the JA signaling pathways in response to abiotic stresses, including cold, drought, salinity, heavy metals, and light. On the other hand, JA does not play an independent regulatory role, but works in a complex signal network with other phytohormone signaling pathways. In this review, we will discuss transcription factors and genes involved in the regulation of the JA signaling pathway in response to abiotic stress. In this process, the JAZ-MYC module plays a central role in the JA signaling pathway through integration of regulatory transcription factors and related genes. Simultaneously, JA has synergistic and antagonistic effects with abscisic acid (ABA), ethylene (ET), salicylic acid (SA), and other plant hormones in the process of resisting environmental stress.

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Molecular Cloning and Functional Characterization of CpMYC2 and CpBHLH13 Transcription Factors from Wintersweet (Chimonanthus praecox L.)

Plants ◽

10.3390/plants9060785 ◽

2020 ◽

Vol 9 (6) ◽

pp. 785

Author(s):

Muhammad Zeshan Aslam ◽

Xiang Lin ◽

Xiang Li ◽

Nan Yang ◽

Longqing Chen

Keyword(s):

Arabidopsis Thaliana ◽

Transcription Factors ◽

Transgenic Plants ◽

Jasmonic Acid ◽

Volatile Compounds ◽

Floral Scent ◽

Functional Characterization ◽

High Expression ◽

Floral Volatile ◽

Chimonanthus Praecox

Wintersweet (Chimonanthus praecox L.) is an ornamental and economically significant shrub known for its unique flowering characteristics, especially the emission of abundant floral volatile organic compounds. Thus, an understanding of the molecular mechanism of the production of these compounds is necessary to create new breeds with high volatile production. In this study, two bHLH transcription factors (CpMYC2 and CpbHLH13) of Wintersweet H29 were functionally characterized to illustrate their possible role in the production of volatile compounds. The qRT-PCR results showed that the expression of CpMYC2 and CpbHLH13 increased from the flower budding to full bloom stage, indicating that these two genes may play an essential role in blooming and aroma production in wintersweet. Gas chromatography-mass spectroscopy (GC-MS) analysis revealed that the overexpression of CpMYC2 in arabidopsis (Arabidopsis thaliana) AtMYC2-2 mutant (Salk_083483) and tobacco (Nicotiana tabaccum) genotype Petit Havana SR1 significantly increased floral volatile monoterpene, especially linalool, while the overexpression of CpbHLH13 in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and tobacco genotype SR1 increased floral sesquiterpene β-caryophyllene production in both types of transgenic plants respectively. High expression of terpene synthase (TPS) genes in transgenic A. thaliana along with high expression of CpMYC2 and CpbHLH13 in transgenic plants was also observed. The application of a combination of methyl jasmonic acid (MeJA) and gibberellic acid (GA3) showed an increment in linalool production in CpMYC2-overexpressing arabidopsis plants, and the high transcript level of TPS genes also suggested the involvement of CpMYC2 in the jasmonic acid (JA) signaling pathway. These results indicate that both the CpMYC2 and CpbHLH13 transcription factors of wintersweet are possibly involved in the positive regulation and biosynthesis of monoterpene (linalool) and sesquiterpene (β-caryophyllene) in transgenic plants. This study also indicates the potential application of wintersweet as a valuable genomic material for the genetic modification of floral scent in other flowering plants that produce less volatile compounds.

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