Removing the major allergen Bra j I from brown mustard ( Brassica juncea ) by CRISPR/Cas9

Bra j IE, a major allergen from oriental-mustard (Brassica juncea) seeds, has been isolated and characterized. Its primary structure has been elucidated. This protein is composed of two chains (37 and 92 amino acids) linked by disulphide bridges. The amino acid sequence obtained is closely related to that previously determined for Sin a I, an allergen isolated from yellow mustard (Sinapis alba). A common epitope has been detected in the large chain of both Bra j IE and Sin a I by means of electroblotting and immunodetection with 2B3, which is a monoclonal antibody raised against the yellow-mustard allergen. A histidine residue of the large chain of both mustard allergens has been found to be essential for the recognition by 2B3 antibody. A synthetic multiantigenic peptide containing this His was recognized by 2B3 as well as by sera of mustard-hypersensitive individuals. Therefore this antigenic determinant must be involved in the allergenicity of these proteins.

Download Full-text

Die GaterslebenerBrassica-Kollektion –Brassica juncea, B. napus, B. nigra undB. rapa

Feddes Repertorium ◽

10.1002/fedr.4921030704 ◽

1992 ◽

Vol 103 (7-8) ◽

pp. 469-507 ◽

Cited By ~ 1

Author(s):

Th. Gladis ◽

K. Hammer

Keyword(s):

Brassica Juncea

Download Full-text

Hypoallergenic recombinant variants of Bet v 1, the major allergen of birch pollen: Tools for specific immunotheraphy of birch pollen allergy?

Immunology Letters ◽

10.1016/s0165-2478(97)87712-7 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 219

Author(s):

C Ebner

Keyword(s):

Birch Pollen ◽

Pollen Allergy ◽

Major Allergen ◽

Bet V 1 ◽

Birch Pollen Allergy

Download Full-text

Variabilitas Genetik dan Heritabilitas Karakter Agronomi Galur-Galur Sawi (Brassica juncea L.)

AGROSAINSTEK Jurnal Ilmu dan Teknologi Pertanian ◽

10.33019/agrosainstek.v3i2.72 ◽

2019 ◽

Vol 3 (2) ◽

pp. 73-83

Author(s):

Raihan Fadhil Muhammad ◽

Budi Waluyo

Keyword(s):

Brassica Juncea ◽

Seed Bank ◽

Augmented Design

Penelitian ini bertujuan untuk mempelajari variabilitas genetik, heritabilitas, dan menyeleksi penampilan genotipe karakter agronomi unggul pada 57 galur sawi untuk digunakan dalam bahan baku konsumsi dan industri. Penelitian ini dilaksanakan di Seed Bank and Nursery, Agrotechno Park Universitas Brawijaya, Desa Jatikerto, Kecamatan Kromengan, Kabupaten Malang pada bulan Desember 2018 – April 2019. Metode yang digunakan untuk penelitian ini adalah rancangan acak kelompok diperluas (augmented design). Perlakuan yang diberikan adalah 60 genotipe sawi yang terdiri dari 57 genotipe yang diuji dan 3 varietas sebagai cek. Genotipe yang diuji akan disebar kedalam 5 blok, sedangkan tiga varietas cek akan ditanam pada setiap blok, sehingga terdapat 72 satuan percobaan. Variabel pengamatan karakter agronomi terdiri dari 15 karakter kualitatif dan 24 karakter kuantitatif. Variabilitas yang luas terdapat pada karakter biji per polong, jumlah polong per tanaman, dan berat segar. Heritabilitas tinggi terdapat pada karakter panjang kotiledon, jumlah daun konsumsi, berat segar, umur panen benih, jumlah polong per tanaman, panjang polong, lebar polong, dan jumlah biji per polong. Terdapat galur-galur sawi yang mempunyai karakter unggul untuk bahan baku konsumsi dan industri.

Download Full-text

ASSESSMENT OF YIELD LOSSES IN MUSTARD ( Brassica juncea L.) DUE TO MUSTARD APHID ( Lipaphis erysimi Kalt.) UNDER DIFFERENT THERMAL ENVIRONMENTS IN EASTERN CENTRAL INDIA

Applied Ecology and Environmental Research ◽

10.15666/aeer/02001015 ◽

2004 ◽

Vol 2 (1) ◽

pp. 1-15 ◽

Cited By ~ 17

Author(s):

S.R. Patel

Keyword(s):

Brassica Juncea ◽

Central India ◽

Lipaphis Erysimi ◽

Yield Losses ◽

Mustard Aphid ◽

Thermal Environments

Download Full-text

Agronomic performance of autumn- and winter-cropped Indian mustard (Brassica juncea L.) in response to varying levels of nitrogen fertiliser

Annales Universitatis Paedagogicae Cracoviensis Studia Naturae ◽

10.24917/25438832.5.6 ◽

2020 ◽

pp. 83-95

Author(s):

Bent Al-Hoda Asghari ◽

Mohsen Yousefi ◽

Katarzyna Możdżeń ◽

Joanna Puła ◽

Peiman Zandi ◽

...

Keyword(s):

Brassica Juncea ◽

Seed Yield ◽

Agronomic Traits ◽

Growth Parameters ◽

Indian Mustard ◽

Climatic Conditions ◽

Plant Seed ◽

Nitrogen Fertiliser ◽

Nitrogen Levels ◽

Cropping Seasons

Indian mustard (Brassica juncea L. Czern) cultivation is suggested for regions with short seasons and low rainfall. Although there have been many studies conducted on agronomic production of mustard in Iran, the information regarding the interactive impact of cropping seasons and nitrogen fertiliser on growth characteristics and yield quality of mustard plant is still insufficient and requires further investigation. This study focused on the possible implications of different cropping seasons and different nitrogen levels on selected agronomic traits in mustard. In this experiment, five different doses of nitrogen and two sowing periods were used to assess for their combined effects on the growth parameters, seed yield and agronomic characteristics of mustard in the semi-arid climatic conditions of Takestan. The results revealed that cultivation seasons and nitrogen rates had a significant effect on plant height, biomass yield, number of siliques per plant, seed oil content and seed yield.

Download Full-text