Epichloë Endophyte-Promoted Seed Pathogen Increases Host Grass Resistance Against Insect Herbivory

Plants host taxonomically and functionally complex communities of microbes. However, ecological studies on plant–microbe interactions rarely address the role of multiple co-occurring plant-associated microbes. Here, we contend that plant-associated microbes interact with each other and can have joint consequences for higher trophic levels. In this study we recorded the occurrence of the plant seed pathogenic fungus Claviceps purpurea and aphids (Sitobion sp.) on an established field experiment with red fescue (Festuca rubra) plants symbiotic to a seed transmitted endophytic fungus Epichloë festucae (E+) or non-symbiotic (E–). Both fungi are known to produce animal-toxic alkaloids. The study was conducted in a semi-natural setting, where E+ and E– plants from different origins (Spain and Northern Finland) were planted in a randomized design in a fenced common garden at Kevo Subarctic Research Station in Northern Finland. The results reveal that 45% of E+ plants were infected with Claviceps compared to 31% of E– plants. Uninfected plants had 4.5 times more aphids than Claviceps infected plants. By contrast, aphid infestation was unaffected by Epichloë symbiosis. Claviceps alkaloid concentrations correlated with a decrease in aphid numbers, which indicates their insect deterring features. These results show that plant mutualistic fungi can increase the infection probability of a pathogenic fungus, which then becomes beneficial to the plant by controlling herbivorous insects. Our study highlights the complexity and context dependency of species–species and multi-trophic interactions, thus challenging the labeling of species as plant mutualists or pathogens.

Effect of Ethephon Application on Seed Yield and Yield Components of Two Faba Bean (Vicia faba L.) Cultivars

The aim of present study was to determine the effects of different ethephon doses on grain yield and yield components of two faba bean cultivars. The experiment was established in 2019/2020 and 2020/2021 cropping season at Research Area of Field Crops Department, Agriculture of Faculty, Cukurova University Adana, Turkey. The field experiment was laid out in randomized complete blocks design (RCBD) with three replications on the basis split plot design with cultivars (Luz de Otono and Histal) in main plots and ethephon doses (0, 500, 1000, 1500 g ha-1) in sub plots. Plant height (cm), branches per plant, pods per plant, seeds per plant, seed yield per plant (g), 100 grain weight (g), seed yield (kg ha-1) were investigated. Differences among the cultivars and ethephon applications were significant for seed yield and it varied from 1782 to 3388 kg ha-1 in the mean of the years. Seed yield also decreased with increasing ethephon doses. Seed yield was higher in 2019/2020 (3355 kg ha) than 2020/2021 (1841 kg ha-1) where low rainfed and high temperature. The present results suggested that ethephon applications at inititation of flowering were not useful for seed production of faba bean.

Intraspecific Competitive Interactions Rapidly Evolve Via Spontaneous Mutations

Using a mechanistic eco-evolutionary trait-based neighborhood-model, we quantify the impact of mutations on intraspecific spatial interactions to better understand mechanisms underlying the maintenance of genetic variation and the potential effects of these evolved interactions on the population dynamics of Arabidopsis thaliana. We use 100 twenty-fifth generation mutation accumulation (MA) lines (genotypes) derived from one founder genotype to study mutational effects on neighbor responses in a field experiment. We created individual-based maps (15,000 individuals), including phenotypic variation, to quantify mutational effects within genotypes versus between genotypes on reproduction and survival. At small-scale (within 80 cm of the focal plant), survival is enhanced but seed-set is decreased when a genotype is surrounded by different genotypes. At large-scale (within 200 cm of the focal plant), seed set is facilitated by different genotypes while the same genotype has either no effect or negative effects. The direction of the interactions among MA lines suggests that at small scale these interactions may contribute to the maintenance of genetic variation and at large scale contribute to the survival of the population. This may suggest, that, mutations potentially have immediate effects on population and community dynamics by influencing the outcome of competitive and faciliatory interactions among conspecifics.

Role of beneficial microbes with nitrogen and phosphorous levels on canola productivity

A research was conducted to evaluate the impact of various nitrogen and phosphorus levels along with beneficial microbes to enhance canola productivity. The research was carried out at Agronomy Research Farm, The University of Agriculture Peshawar in winter 2016-2017. The experiment was conducted in randomized complete block factorial design. The study was comprised of three factors including nitrogen (60, 120 and 180 kg ha-1), phosphorous (70, 100 and 130 kg ha-1) and beneficial microbes (with and without BM). A control treatment with no N, P and BM was also kept for comparison. Application of beneficial microbes significantly increased pods plant, seed pod, seed filling duration, 1000 seed weight, biological yield and seed yield as compared to control plots. Nitrogen applied at the rate of 180 kg ha-1 increased pods plant-1, seed pod, seed filling duration, seed weight, biological yield and seed yield. Maximum pods plant-1, seed pod, early seed filling, heavier seed weight, biological yield, seed yield, and harvest index were observed in plots treated with 130 kg.ha-1 phosphorous. As comparison, the combine treated plots have more pods plant-1, seeds pod-1, seed filling duration, heaviest seeds, biological yield, seed yield and harvest index as compared to control plots. It is concluded that application of beneficial microbes with N and P at the rate of 180 kg ha-1 and 130 kg ha-1, respectively, increased yield and its attributes for canola.

Yield components of the M7 Kipas Putih mutant soybean

This study aimed to obtain the homogeny of M8 soybean lines in an effort to release new high yielding varieties. The research was conducted in Lamsidaya village, and the observation of yield components was carried out at the Plant Breeding Laboratory, Faculty of Agriculture, Universitas Syiah Kuala. This study did not use an experimental design, so that the quantitative characters were observed individually from each plant, then analyzed by estimating the heritability broad sense value and the progress of selection. The material used were var. Kipas Putih and var. Dega1 as control, eight of 7th generation Kipas Putih mutant lines (B4, B7, B10, B12, B13, B15, B18 and B22). All genotypes were planted in rectangular plot 10 m x 1 m, drainage 50 cm, and planting distance 50 cm x 30 cm. Plants were maintained in accordance with the recommendation for soybean cultivation from Balitkabi, and fertilizer NPK (16:16:16) was given 200 g/plot (200 kgha-1). Parameters observed included plant height, flowering time, harvest time, number of productive branches, total number of pods per plant, number of filled pods per plant, percentage of filled pods per plant, seed weight per plant, weight of 100 seeds, seed weight per plot, and yield potential. All quantitative characters indicated increase on selection except for the character of plant height, percentage of filled pods and weight of 100 seeds because the heritability value was low. The B18 line has the potential to become a new high yielding variety because it has a high yield potential (3.02 tons ha-1) with a large seed size compared to the other seven mutant lines and is also higher than the parent Kipas Putih and var Dega 1 varieties.

The application of ammonium sulphate and amino acid on cotton: effects on can improve growth, yield, quality and nitrogen absorption

A field study was carried out to determine the influence of foliage applied plant growth promoter and retardant in improving soil applied sulphur fertilizer use efficiency in cotton during two consecutive summers 2014 and 2015. Experimental trial comprised of three different sources of sulphur (ammonium sulphate, potassium sulphate and elemental sulphur) and foliar spray of plant growth promoter and growth retardant including tap water was taken as control. Among treatments soil applied ammonium sulphate with foliage applied amino acid produced maximum plant height, sympodial branches, pods per plant, seed cotton yield, fiber yield, biological yield, protein contents, oil contents and leaf nitrogen uptake as compared to the other treatments. Whereas, soil applied potassium sulphate with foliar spray of mepiquat chloride on cotton significantly improved the boll weight and leaf potassium uptake. We conclude that soil applied ammonium sulphate and foliage spray of amino acid was more effective in improving the productivity and quality attributes of cotton.

The Effect of Volatile Organic Compounds on Different Organisms: Agrobacteria, Plants and Insects

Bacteria and fungi emit a huge variety of volatile organic compounds (VOCs) that can provide a valuable arsenal for practical use. However, the biological activities and functions of the VOCs are poorly understood. This work aimed to study the action of individual VOCs on the bacteria Agrobacterium tumefaciens, Arabidopsis thaliana plants, and fruit flies Drosophila melanogaster. VOCs used in the work included ketones, alcohols, and terpenes. The potent inhibitory effect on the growth of A. tumefaciens was shown for 2-octanone and isoamyl alcohol. Terpenes (−)-limonene and (+)-α-pinene practically did not act on bacteria, even at high doses (up to 400 µmol). 2-Butanone and 2-pentanone increased the biomass of A. thaliana at doses of 200–400 μmol by 1.5–2 times; 2-octanone had the same effect at 10 μmol and decreased plant biomass at higher doses. Isoamyl alcohol and 2-phenylethanol suppressed plant biomass several times at doses of 50–100 μmol. Plant seed germination was most strongly suppressed by isoamyl alcohol and 2-phenylethanol. The substantial killing effect (at low doses) on D. melanogaster was exerted by the terpenes and the ketones 2-octanone and 2-pentanone. The obtained data showed new information about the biological activities of VOCs in relation to organisms belonging to different kingdoms.

Agro-Morphological Characterization and Some Seed Characteristics of Wild Crambe (Brassicaceae) Species in Turkey

The seeds of wild Crambe species have potential to be used as a source of industrial oil and animal feed. In this study, 48 genotypes of three Crambe species collected from the flora of Turkey were grown under field conditions in Ankara/Turkey in 2014–2016. The seed protein ratio, plant height, number of branches per plant, number of seeds per plant, seed yield per plant, thousand seed weight and hulless/hulled seed ratio (H/H) were determined. The highest protein ratio was determined as 26.02% in the t18 accession of Crambe tataria species. Variations in the characteristics were analyzed using principal component analysis. In the factor analysis of Crambe maritima, Crambe orientalis, Crambe tataria and the mean of these three species, the first two principal components accounted for 100%, 58.06%, 59.93% and 100% of the total variations, respectively. There were positive correlations between the plant height and number of seeds per plant, seed yield per plant for C. orientalis, and number of branches per plant for C. tataria. Although seed yield per plant was high in C. tataria and C. orientalis, they are not suitable for conventional agriculture due to shell thickness, inhomogeneous plant emergence and shooting. Conventional cultivation of wild Crambe species can be made possible by eliminating these negative features with breeding and agronomic studies.