scholarly journals Induction of suppressive allogeneic regulatory T cells via rabbit antithymocyte polyclonal globulin during homeostatic proliferation in rat kidney transplantation

2014 ◽  
Vol 28 (1) ◽  
pp. 108-119 ◽  
Author(s):  
Rafael Valdez-Ortiz ◽  
Oriol Bestard ◽  
Inés Llaudó ◽  
Marcella Franquesa ◽  
Gema Cerezo ◽  
...  
Keyword(s):  
T Cells ◽  
Kidney Transplantation ◽  
Regulatory T Cells ◽  
Rat Kidney ◽  
Homeostatic Proliferation

scholarly journals Regulatory T Cells Fail to Suppress Fast Homeostatic Proliferation In Vitro

Life ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 245
Author(s):  
Daniil Shevyrev ◽  
Valeriy Tereshchenko ◽  
Elena Blinova ◽  
Nadezda Knauer ◽  
Ekaterina Pashkina ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
T Cell ◽  
Regulatory T Cells ◽  
Autoimmune Diseases ◽  
Peripheral Blood ◽  
Treg Cells ◽  
Homeostatic Proliferation ◽  
Suppressive Activity ◽  
Healthy Donors

Homeostatic proliferation (HP) is a physiological process that reconstitutes the T cell pool after lymphopenia involving Interleukin-7 and 15 (IL-7 and IL-15), which are the key cytokines regulating the process. However, there is no evidence that these cytokines influence the function of regulatory T cells (Tregs). Since lymphopenia often accompanies autoimmune diseases, we decided to study the functional activity of Tregs stimulated by HP cytokines from patients with rheumatoid arthritis as compared with that of those from healthy donors. Since T cell receptor (TCR) signal strength determines the intensity of HP, we imitated slow HP using IL-7 or IL-15 and fast HP using a combination of IL-7 or IL-15 with anti-CD3 antibodies, cultivating Treg cells with peripheral blood mononuclear cells (PBMCs) at a 1:1 ratio. We used peripheral blood from 14 patients with rheumatoid arthritis and 18 healthy volunteers. We also used anti-CD3 and anti-CD3 + IL-2 stimulation as controls. The suppressive activity of Treg cells was evaluated in each case by the inhibition of the proliferation of CD4+ and CD8+ cells. The phenotype and proliferation of purified CD3+CD4+CD25+CD127lo cells were assessed by flow cytometry. The suppressive activity of the total pool of Tregs did not differ between the rheumatoid arthritis and healthy donors; however, it significantly decreased in conditions close to fast HP when the influence of HP cytokines was accompanied by anti-CD3 stimulation. The Treg proliferation caused by HP cytokines was lower in the rheumatoid arthritis (RA) patients than in the healthy individuals. The revealed decrease in Treg suppressive activity could impact the TCR landscape during lymphopenia and lead to the proliferation of potentially self-reactive T cell clones that are able to receive relatively strong TCR signals. This may be another explanation as to why lymphopenia is associated with the development of autoimmune diseases. The revealed decrease in Treg proliferation under IL-7 and IL-15 exposure can lead to a delay in Treg pool reconstitution in patients with rheumatoid arthritis in the case of lymphopenia.

ACCELERATED REJECTION OF ALLOGRAFTS FOLLOWING HOMEOSTATIC PROLIFERATION OF T CELLS DESPITE DISPROPORTIONATE EXPANSION OF REGULATORY T CELLS

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 258-259
Author(s):  
W Wong ◽  
K Brown ◽  
T Tapmeier ◽  
V Moxham ◽  
J Karegli ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Homeostatic Proliferation

Do Regulatory T Cells Play a Role in the Control of Homeostatic Proliferation?

2005 ◽  
Vol 24 (3-4) ◽  
pp. 269-284 ◽  
Author(s):  
Maria A. Curotto de Laffaille ◽  
Shiqian Shen ◽  
Danyvid Olivares-Villagómez ◽  
Marlin Camps-Ramírez ◽  
Juan J. Lafaille
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Homeostatic Proliferation

scholarly journals Ex Vivo Costimulatory Blockade to Generate Regulatory T Cells From Patients Awaiting Kidney Transplantation

2016 ◽  
Vol 16 (7) ◽  
pp. 2187-2195 ◽  
Author(s):  
E. C. Guinan ◽  
G. A. Cole ◽  
W. H. Wylie ◽  
R. H. Kelner ◽  
K. J. Janec ◽  
...  
Keyword(s):  
T Cells ◽  
Kidney Transplantation ◽  
Regulatory T Cells ◽  
Ex Vivo ◽  
Costimulatory Blockade

Discontinuation of calcineurin inhibitors treatment allows the development of FOXP3+ regulatory T-cells in patients after kidney transplantation

2011 ◽  
Vol 25 (1) ◽  
pp. 40-46 ◽  
Author(s):  
Jacqueline van de Wetering ◽  
Periklis Koumoutsakos ◽  
Annemiek Peeters ◽  
Barbara J. van der Mast ◽  
Petronella de Kuiper ◽  
...  
Keyword(s):  
T Cells ◽  
Kidney Transplantation ◽  
Regulatory T Cells ◽  
Calcineurin Inhibitors

scholarly journals Central Role of CD45RA− Foxp3hi Memory Regulatory T Cells in Clinical Kidney Transplantation Tolerance

2015 ◽  
Vol 26 (8) ◽  
pp. 1795-1805 ◽  
Author(s):  
Faouzi Braza ◽  
Emilie Dugast ◽  
Ivo Panov ◽  
Chloé Paul ◽  
Katrin Vogt ◽  
...  
Keyword(s):  
T Cells ◽  
Kidney Transplantation ◽  
Regulatory T Cells ◽  
Transplantation Tolerance

scholarly journals Control of homeostatic proliferation by regulatory T cells

2005 ◽  
Vol 115 (12) ◽  
pp. 3517-3526 ◽  
Author(s):  
S. Shen
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Homeostatic Proliferation

ANTI-THYMOCYTE GLOBULIN INDUCTION FAVORS THE GENERATION OF FUNCTIONAL CD4+FOXP3+CD127-/LOW REGULATORY T CELLS IN PATIENTS AFTER KIDNEY TRANSPLANTATION

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 72
Author(s):  
V Sewgobind ◽  
M Kho ◽  
L van der Laan ◽  
T van Dam ◽  
H Tilanus ◽  
...  
Keyword(s):  
T Cells ◽  
Kidney Transplantation ◽  
Regulatory T Cells

scholarly journals Transient increase of activated regulatory T cells early after kidney transplantation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Young-Seon Mederacke ◽  
Florian W. Vondran ◽  
Sonja Kollrich ◽  
Elvira Schulde ◽  
Roland Schmitt ◽  
...  
Keyword(s):  
T Cells ◽  
Kidney Transplantation ◽  
Regulatory T Cells ◽  
Transient Increase
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