Abstract
Objective: To investigate the effect and mechanisms of Andira anthelmia lectin in rat models of acute inflammation.Material: AAL anti-inflammatory activity was evaluated in Wistar rat models of paw edema, peritonitis and hyperalgesia.Methods: AAL (0.01-1 mg/kg i.v.) was injected 30 min before stimulation with carrageenan and with initial and late phase inflammatory mediators into the animals paw and peritoneum for evaluation of: cell migration (optical and intravital microscopy), paw edema (plethysmometry and histopathology); hyperalgesia (analgesimetry).Results: AAL inhibited leukocyte migration induced by carrageenan, mainly neutrophils to the peritoneal fluid, decreasing leukocyte adhesion. In the peritoneal fluid, AAL reduced the gene expression of TNF-α and cyclooxygenase, as well the levels of PGE2. ALL also inhibited the paw edema induced by carrageenan, mainly the late phase, but also the edema induced by serotonin, histamine, TNF-α, PLA2 and PGE2, but not by L-arginine and bradykinin. In this model, AAL inhibited mechanical hypernociception induced by TNF-α, PGE2, db-cAMP and capsaicin, and the activity of myeloperoxidase in the paw tissues.Conclusion: AAL presents anti-inflammatory effect in acute models of rat inflammation involving the participation of prostaglandins, TNF-α and lectin domain.
Anti-inflammatory effect of non-thermal atmospheric pressure plasma for periodontitis treatment: in vitro pilot study