Identification and characterization of drought responsive miRNAs in a drought tolerant upland rice cultivar KMJ 1-12-3

2019 ◽  
Vol 137 ◽  
pp. 62-74 ◽  
Author(s):  
Jay Prakash Awasthi ◽  
Tilak Chandra ◽  
Sagarika Mishra ◽  
Shaifaly Parmar ◽  
Birendra Prasad Shaw ◽  
...  
2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Teame Gereziher MEHARI ◽  
Yanchao XU ◽  
Richard Odongo MAGWANGA ◽  
Muhammad Jawad UMER ◽  
Joy Nyangasi KIRUNGU ◽  
...  

Abstract Background Cotton is an important commercial crop for being a valuable source of natural fiber. Its production has undergone a sharp decline because of abiotic stresses, etc. Drought is one of the major abiotic stress causing significant yield losses in cotton. However, plants have evolved self-defense mechanisms to cope abiotic factors like drought, salt, cold, etc. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), and the late embryogenesis abundant proteins have shown positive response in the resistance improvement to several abiotic stresses. Results Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes were carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed on various chromosomes. The Ka/Ks (Non-synonymous substitution rate/Synonymous substitution rate) values were less than one, an indication of negative selection of the gene family. Differential expressions of genes showed that majority of the genes are being highly upregulated in the roots as compared with leaves and stem tissues. Most genes were found to be highly expressed in MR-85, a relative drought tolerant germplasm. Conclusion The results provide proofs of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton varieties.


2017 ◽  
Vol 54 (3) ◽  
pp. 253 ◽  
Author(s):  
Vinay Sharma ◽  
Rahul K Verma ◽  
PC Dey ◽  
SK Chetia ◽  
AR Baruah ◽  
...  

2021 ◽  
Vol 83 (4) ◽  
pp. 137-150
Author(s):  
Nurul Fairuz Diyana Bahrudin ◽  
Umar Hamzah ◽  
Wan Zuhairi Wan Yaacob

The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols.  Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars.  This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah.  For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6).  To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L.  To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours).  2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively.   The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus.  The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively.   Shoots developed into complete plants on hormone-free MS medium and acclimatized. 


Author(s):  
Fernando de S. Buzo ◽  
Lucas M. Garé ◽  
Orivaldo Arf ◽  
José R. Portugal ◽  
Flavia C. Meirelles ◽  
...  

ABSTRACT In rice, the use of the bacterium Azospirillum brasilense has shown to be very useful for the crop, being able to increase the final productivity of the same or reduce the need for nitrogen in topdressing. It has recently been noted that there are other regulators that perform other functions, such as thidiazuron (TDZ), a cytokinin-like substance that has been shown to significantly increase upland rice productivity. Thus, the present work aimed to verify the effect of the interaction between the foliar application of A. brasilense and thidiazuron doses applied at the occasion of the tillering of the plants on the productive aspects and crop productivity. The experiment was carried out in the agricultural years of 2015/16 and 2016/17, at the UNESP Teaching, Research and Extension Farm belonging to the Engineering Faculty of Ilha Solteira - UNESP, located in the municipality of Selvíria, MS. The experimental design was a randomized complete block design with a 2 x 4 factorial scheme with four replicates. The treatments were the combination of the presence or absence of A. brasilense foliar application with doses of TDZ (0, 0.5, 1.0 and 1.5 g ha-1) applied to upland rice cultivar BRS Esmeralda. Based on the results obtained for plant height, number of panicles per square meter, total number of grains and fertility of spikelets, mass of 100 grains and grain yield, it was concluded that, in the two agricultural years observed, there were no interaction and individual effects of the foliar application of the bacteria, and of the TDZ doses used at the time of tillering in the BRS Esmeralda cultivar.


2010 ◽  
Vol 10 (2) ◽  
pp. 176-179 ◽  
Author(s):  
A.A. Soares ◽  
M.S. Reis ◽  
V.M.O. Cornelio ◽  
P.C. Soares ◽  
G.T. Costa Júnior ◽  
...  

2013 ◽  
Vol 13 (3) ◽  
pp. 208-211 ◽  
Author(s):  
Antônio Alves Soares ◽  
Moizés de Sousa Reis ◽  
Vanda Maria de Oliveira Cornélio ◽  
Natália Alves Leite ◽  
Plínio César Soares ◽  
...  

2017 ◽  
Vol 14 (3) ◽  
pp. 1051-1054 ◽  
Author(s):  
Budianti Kadidaa ◽  
Gusti R. Sadimantara ◽  
Suaib Suaib ◽  
La Ode Safuan ◽  
Muhidin Muhidin

ABSTRACT: Demand for rice as a source of food in Indonesia continues to increase in line with the growth of population, while the capacity to produce paddy rice increasingly limited. One of the efforts is to develop upland rice by utilizing the dry land with organic fertilizer application. The research was conducted in North Buton using split plot design. The main plot was the treatment of manure dosage consisting of 4 levels of treatment and the second factor of local upland rice cultivars as a subplot consisting of 22 cultivars. Production characters were observed and assessments are panicle length, grain number, number of grain content, wet grain weight, dried grain weight, grain weight per culm, and grain yield (t ha-1). The results showed that the yield potency of local upland rice cultivar in North Buton ranged from 3.11- 4.97 t ha-1 and the treatment of organic fertilizer can increase the yield components of upland rice.


2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Paulo Hideo Nakano Rangel ◽  
Adriano Pereira Castro ◽  
Mabio Crisley Lacerda ◽  
Isabela Volpi Furtini ◽  
Daniel de Brito Fragoso ◽  
...  

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